Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,769,648 (C)6→5 intergenic (+162/‑55) yfjQ → / → yfjR CP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,769,6430C.96.2% 194.3 / 2.3 52intergenic (+157/‑60)yfjQ/yfjRCP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base . (26/24);  minor base A (1/1);  total (27/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.26e-01

GTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGT  >  NC_000913/2769507‑2769777
                                                                                                                                        |                                                                                                                                      
gttAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGccc                                                                                                                                      <  1:939708/139‑1 (MQ=255)
 ttAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTAGATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCCGTCCGcccc                                                                                                                                     <  2:773117/139‑1 (MQ=255)
 ttAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGcccc                                                                                                                                     <  2:1041347/139‑1 (MQ=255)
               caccTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGcccc                                                                                                                                     <  1:113226/125‑1 (MQ=255)
               caccTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGcccc                                                                                                                                     >  2:113226/1‑125 (MQ=255)
               caccTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCttt                                                                                                                              <  2:293462/131‑1 (MQ=255)
               caccTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCttt                                                                                                                              >  1:293462/1‑131 (MQ=255)
                  cTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATcac                                                                                                                   >  1:1203916/1‑139 (MQ=255)
                         cATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAACAATAGTTTCTGCGTTGTCCATACCCTGTCCGccccctctt                                                                                                                                >  1:739196/1‑116 (MQ=255)
                         cATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGccccctctt                                                                                                                                <  2:739196/120‑5 (MQ=255)
                                       cGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTtcagtcag                                                                                                    <  2:431420/133‑1 (MQ=255)
                                       cGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTtcagtcag                                                                                                    >  1:431420/1‑133 (MQ=255)
                                           tgCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCTTTTTCAGTCAGTTaa                                                                                                <  1:96182/133‑1 (MQ=255)
                                           tgCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCTTTTTCAGTCAGTTaa                                                                                                >  2:96182/1‑133 (MQ=255)
                                             ccTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGcccc                                                                                                                                     <  2:391273/95‑1 (MQ=255)
                                             ccTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGcccc                                                                                                                                     >  1:391273/1‑95 (MQ=255)
                                             ccTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTaa                                                                                                                            <  2:1200253/103‑1 (MQ=255)
                                             ccTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTaa                                                                                                                            >  1:1200253/1‑103 (MQ=255)
                                             ccTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTaa                                                                                                <  1:968201/131‑1 (MQ=255)
                                             ccTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTaa                                                                                                >  2:968201/1‑131 (MQ=255)
                                                   tCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAAt                                                                                  <  2:1203916/139‑1 (MQ=255)
                                                             ataGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGac                                                                        <  2:511521/139‑1 (MQ=255)
                                                               aGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGacac                                                                      <  2:206745/139‑1 (MQ=255)
                                                               aGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGacac                                                                      >  2:359904/1‑139 (MQ=255)
                                                                    ttAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTaa                                                                                                <  1:234706/108‑1 (MQ=255)
                                                                    ttAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTaa                                                                                                <  2:693985/108‑1 (MQ=255)
                                                                    ttAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTaa                                                                                                >  1:693985/1‑108 (MQ=255)
                                                                    ttAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTaa                                                                                                >  2:234706/1‑108 (MQ=255)
                                                                    ttAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCa                                                                 >  1:535220/1‑139 (MQ=255)
                                                                     tAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTaa                                                                                                >  2:597157/1‑107 (MQ=255)
                                                                     tAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTaa                                                                                                <  1:597157/107‑1 (MQ=255)
                                                                      aaCCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTaa                                                                                                >  2:400205/1‑106 (MQ=255)
                                                                      aaCCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTaa                                                                                                <  1:400205/106‑1 (MQ=255)
                                                                            cGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGaa                                                                                   <  1:688291/113‑1 (MQ=255)
                                                                            cGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGaa                                                                                   >  2:688291/1‑113 (MQ=255)
                                                                                   tCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTACGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGAc                                                  <  2:771508/139‑1 (MQ=255)
                                                                                      ggCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGcccc                                                                                                                                     >  2:470058/1‑54 (MQ=255)
                                                                                      ggCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGcccc                                                                                                                                     <  1:470058/54‑1 (MQ=255)
                                                                                      ggCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTaa                                                                                                <  2:441890/90‑1 (MQ=255)
                                                                                      ggCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTaa                                                                                                >  1:441890/1‑90 (MQ=255)
                                                                                       gCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCggc                                              >  1:835273/1‑139 (MQ=255)
                                                                                       gCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCggc                                              <  2:835273/139‑1 (MQ=255)
                                                                                       gCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCACCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAAc                                                             >  2:115276/1‑124 (MQ=255)
                                                                                       gCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCACCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAAc                                                             <  1:115276/124‑1 (MQ=255)
                                                                                        cTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATc                                                                                                                     <  2:151911/67‑1 (MQ=255)
                                                                                        cTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATc                                                                                                                     >  1:151911/1‑67 (MQ=255)
                                                                                                 aaaTAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGAcc                                                 >  1:643504/1‑126 (MQ=255)
                                                                                                 aaaTAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGAcc                                                 <  2:643504/126‑1 (MQ=255)
                                                                                                  aaTAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTggctgtcagg                                   <  2:500498/139‑1 (MQ=255)
                                                                                                  aaTAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTggctgtcagg                                   <  2:535220/139‑1 (MQ=255)
                                                                                                      aaaaaTAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAATCAGTTAACTTTCCTGGAGAATCTCTCATGACACACGCAGAACGCCGCCATGACCGGCTtgctgtcaggctgt                               <  1:816115/139‑1 (MQ=14)
                                                                                                      aaaaaTAGTTTCTGCATTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTggctgtcaggctgt                               <  2:510552/139‑1 (MQ=255)
                                                                                                           tAGTTTCTGCGTTGTCCATACCCTATCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTg                          >  2:1184476/1‑139 (MQ=255)
                                                                                                               ttCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATaa                      >  2:973894/1‑139 (MQ=255)
                                                                                                                                   gTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGga                                                                                      >  2:530343/1‑55 (MQ=255)
                                                                                                                                   gTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGga                                                                                      <  1:530343/55‑1 (MQ=255)
                                                                                                                                   gTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt  >  2:482658/1‑139 (MQ=255)
                                                                                                                                   gTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt  <  2:127616/139‑1 (MQ=255)
                                                                                                                                   gTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt  >  1:56899/1‑139 (MQ=255)
                                                                                                                                   gTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt  >  1:38857/1‑139 (MQ=255)
                                                                                                                                   gTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt  >  1:127616/1‑139 (MQ=255)
                                                                                                                                   gTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt  >  1:1149637/1‑139 (MQ=255)
                                                                                                                                   gTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGATGCggtt  >  1:782367/1‑137 (MQ=255)
                                                                                                                                        |                                                                                                                                      
GTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGT  >  NC_000913/2769507‑2769777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: