Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,012,553 Δ1 bp intergenic (+160/+61) yqeA → / ← yqeB putative amino acid kinase/XdhC‑CoxI family protein with NAD(P)‑binding Rossman fold

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,012,5520G.100.0% 252.7 / NA 62intergenic (+159/+62)yqeA/yqeBputative amino acid kinase/XdhC‑CoxI family protein with NAD(P)‑binding Rossman fold
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (35/27);  total (35/27)

TCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTAACGCCTCAAGTACG  >  NC_000913/3012419‑3012686
                                                                                                                                     |                                                                                                                                      
tCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAAtt                                                                                                                                  <  1:394176/139‑1 (MQ=255)
              gTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTgg                                                                                                                               >  2:917234/1‑128 (MQ=255)
              gTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTgg                                                                                                                               <  1:917234/128‑1 (MQ=255)
                 aagtaagtGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGt                                                                                                                 <  1:664829/139‑1 (MQ=255)
                                    aTGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGcc                                                                                                         >  2:1065558/1‑128 (MQ=255)
                                    aTGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGcc                                                                                                         <  1:1065558/128‑1 (MQ=255)
                                           aTGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTgg                                                                                                                               <  2:257784/99‑1 (MQ=255)
                                           aTGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTgg                                                                                                                               >  1:257784/1‑99 (MQ=255)
                                           aTGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATcc                                                                                       >  2:390624/1‑139 (MQ=255)
                                            tGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCg                                                                                      <  1:390624/139‑1 (MQ=255)
                                                gcgcGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCa                                                                                  >  2:148937/1‑139 (MQ=255)
                                                 cgcgAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGAGAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCTGCCAt                                                                                 >  2:757823/1‑139 (MQ=255)
                                                      gcgcCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCgggtg                                                                                                    <  2:357787/115‑1 (MQ=255)
                                                      gcgcCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCgggtg                                                                                                    >  1:357787/1‑115 (MQ=255)
                                                          cTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTa                                                                        >  1:1209116/1‑139 (MQ=255)
                                                                cGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCAt                                                                                         <  2:16251/116‑1 (MQ=255)
                                                                cGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCAt                                                                                         >  1:16251/1‑116 (MQ=255)
                                                                  tCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACtt                                                                >  2:1145634/1‑139 (MQ=255)
                                                                  tCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACtt                                                                >  2:977886/1‑139 (MQ=255)
                                                                   ccTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTc                                                               <  2:1209116/139‑1 (MQ=255)
                                                                       cAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                       >  2:17644/1‑95 (MQ=255)
                                                                       cAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                       <  1:17644/95‑1 (MQ=255)
                                                                          aaCATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCTGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCAct                                                        >  2:930494/1‑139 (MQ=255)
                                                                               cGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAgg                                                                                                           <  1:577765/83‑1 (MQ=255)
                                                                               cGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAgg                                                                                                           >  2:577765/1‑83 (MQ=255)
                                                                               cGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCttt                                                   >  1:667321/1‑139 (MQ=255)
                                                                                gCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGttt                                                                                                                          >  2:336942/1‑67 (MQ=255)
                                                                                gCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGttt                                                                                                                          <  1:336942/67‑1 (MQ=255)
                                                                                 cAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTg                                                 >  1:671601/1‑139 (MQ=255)
                                                                                          tAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCa                                        <  1:930494/139‑1 (MQ=255)
                                                                                               tGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCtttt                                                  <  2:707190/124‑1 (MQ=255)
                                                                                               tGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGccc                                   >  1:1042513/1‑139 (MQ=255)
                                                                                               tGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGccc                                   >  1:1203024/1‑139 (MQ=255)
                                                                                               tGCAATGCACTAGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTATCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCtttt                                                  >  1:707190/1‑124 (MQ=255)
                                                                                                gCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCt                                  >  1:535304/1‑139 (MQ=255)
                                                                                                    tGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCa                                        >  1:270321/1‑129 (MQ=255)
                                                                                                    tGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCa                                        <  2:270321/129‑1 (MQ=255)
                                                                                                      cACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCa                            >  1:430389/1‑139 (MQ=255)
                                                                                                      cACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCa                            >  1:542831/1‑139 (MQ=255)
                                                                                                       aCTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCAt                           <  1:1145634/139‑1 (MQ=255)
                                                                                                        cTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAgg                                                                                                           >  2:852344/1‑58 (MQ=17)
                                                                                                        cTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAgg                                                                                                           <  1:852344/58‑1 (MQ=17)
                                                                                                         ttGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCa                         <  2:671601/139‑1 (MQ=255)
                                                                                                         ttGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCa                         <  1:977886/139‑1 (MQ=255)
                                                                                                                 cTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCAttt                                                                               >  1:220077/1‑77 (MQ=255)
                                                                                                                 cTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCAttt                                                                               <  2:220077/77‑1 (MQ=255)
                                                                                                                 cTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCTAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCAtt                 >  1:458989/1‑139 (MQ=255)
                                                                                                                 cTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCAtt                 >  2:163959/1‑139 (MQ=255)
                                                                                                                 cTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCAtt                 <  1:148937/139‑1 (MQ=255)
                                                                                                                   gATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCaa                        >  2:582100/1‑130 (MQ=255)
                                                                                                                   gATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCaa                        <  1:582100/130‑1 (MQ=255)
                                                                                                                   gATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTaa               <  2:542831/139‑1 (MQ=255)
                                                                                                                   gATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTaa               >  2:611365/1‑139 (MQ=255)
                                                                                                                     tAAGCGTAGCGCATCAGCAATTTGGCGTTTGtcat                                                                                                                     >  1:1131058/1‑35 (MQ=14)
                                                                                                                     tAAGCGTAGCGCATCAGCAATTTGGCGTTTGtcat                                                                                                                     <  2:1131058/35‑1 (MQ=14)
                                                                                                                           tAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTaa                                                                                               >  2:1110611/1‑51 (MQ=255)
                                                                                                                           tAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTaa                                                                                               <  1:1110611/51‑1 (MQ=255)
                                                                                                                              cgcATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCAttt                                                                               >  2:1026305/1‑64 (MQ=255)
                                                                                                                              cgcATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCAttt                                                                               <  1:1026305/64‑1 (MQ=255)
                                                                                                                              cgcATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTAACGCCTCAAGTa    >  1:617863/1‑139 (MQ=255)
                                                                                                                              cgcATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTAACGCCTCAAGTa    >  1:788265/1‑139 (MQ=255)
                                                                                                                                cATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTAACGCCTCAAGTAcg  <  2:667321/139‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                      
TCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTAACGCCTCAAGTACG  >  NC_000913/3012419‑3012686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: