Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 321,564 | (G)7→6 | intergenic (+483/‑44) | rclR → / → ykgE | reactive chlorine species (RCS)‑specific activator of the rcl genes/cysteine‑rich LutA family protein, putative electron transport chain YkgEFG component |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 321,558 | 0 | G | . | 98.2% | 225.1 / ‑2.2 | 56 | intergenic (+477/‑50) | rclR/ykgE | reactive chlorine species (RCS)‑specific activator of the rcl genes/cysteine‑rich LutA family protein, putative electron transport chain YkgEFG component |
Reads supporting (aligned to +/- strand): ref base G (0/0); major base . (28/27); minor base T (1/0); total (29/27) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 |
ATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGAT‑CCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAA > NC_000913/321422‑321690 | aTGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGAT‑CCCCCGGTggg > 1:1139509/1‑139 (MQ=255) aaTAAAATTAGTTATTGCATTTGATGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGAT‑CCCCCGGT‑GGGGGGAAACATCg > 2:531905/1‑139 (MQ=255) ttGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGAT‑CCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCa < 2:1139509/139‑1 (MQ=255) tGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGAT‑CCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTa > 2:900226/1‑139 (MQ=255) aCGGTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGAT‑CCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAAt < 2:903248/139‑1 (MQ=255) gTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGAT‑CCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATgg < 2:565041/139‑1 (MQ=255) ttGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGAT‑CCCCCGGT‑GGGGGGAAACAt > 1:703846/1‑110 (MQ=255) ttGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGAT‑CCCCCGGT‑GGGGGGAAACAt < 2:703846/110‑1 (MQ=255) tGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGAT‑CCCCCGGT‑GGGGGGAAACATCGGGCATaaa > 2:1052966/1‑119 (MQ=255) tGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGAT‑CCCCCGGT‑GGGGGGAAACATCGGGCATaaa < 1:1052966/119‑1 (MQ=255) ttGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGAT‑CCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCaa < 1:900226/139‑1 (MQ=255) gTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGAT‑CCCCCGGT‑GGGGGGAAACa > 1:1153209/1‑88 (MQ=255) gTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGAT‑CCCCCGGT‑GGGGGGAAACa < 2:1153209/88‑1 (MQ=255) aaTGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGAT‑CCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTc < 2:512307/139‑1 (MQ=255) aaTGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGAT‑CCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTc > 1:721758/1‑139 (MQ=255) aaTGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGAT‑CCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTc < 1:622885/139‑1 (MQ=255) gAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGAT‑CCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAcc < 1:531905/139‑1 (MQ=255) gAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGAT‑CCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAcc < 2:721758/139‑1 (MQ=255) aGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGAT‑CCCCCCGGTGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAcc > 1:1090533/1‑139 (MQ=255) gggTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGAT‑CCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTaa > 2:317071/1‑99 (MQ=255) gggTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGAT‑CCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTaa < 1:317071/99‑1 (MQ=255) gggTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGAT‑CCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGt > 1:1209539/1‑139 (MQ=255) gtggtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGAT‑CCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATg < 1:1031415/91‑1 (MQ=255) gtggtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGAT‑CCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATg > 2:1031415/1‑91 (MQ=255) tggtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGAT‑CCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAAt > 2:1003043/1‑97 (MQ=255) tggtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGAT‑CCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAAt < 1:1003043/97‑1 (MQ=255) tGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGAT‑CCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGc > 1:370353/1‑139 (MQ=255) gCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGAT‑CCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGcc > 2:882183/1‑139 (MQ=255) aGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGAT‑CCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCa > 2:550748/1‑76 (MQ=255) aGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGAT‑CCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCa < 1:550748/76‑1 (MQ=255) gCCAATTAAAAAAGAACGCCATATTTATTGATGATTGAT‑CCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAcc > 1:1115200/1‑120 (MQ=255) gCCAATTAAAAAAGAACGCCATATTTATTGATGATTGAT‑CCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAcc < 2:1115200/120‑1 (MQ=255) ccAATTAAAAAAGAACGCCATATTTATTGATGATTGAT‑CCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGaa > 2:6946/1‑139 (MQ=255) aTTAAAAAAGAACGCCATATTTATTGATGATTGAT‑CCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCa < 2:65750/71‑1 (MQ=255) aTTAAAAAAGAACGCCATATTTATTGATGATTGAT‑CCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCa > 1:65750/1‑71 (MQ=255) ttAAAAAAGAACGCCATATTTATTGATGATTGAT‑CCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCa > 2:701330/1‑139 (MQ=255) ttAAAAAAGAACGCCATATTTATTGATGATTGAT‑CCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCa > 2:1168044/1‑139 (MQ=255) aaaaaaGAACGCCATATTTATTGATGATTGAT‑CCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAg < 1:6946/139‑1 (MQ=255) aaaaaGAACGCCATATTTATTGATGATTGATCCCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAg < 2:1090533/139‑1 (MQ=255) aaCGCCATATTTATTGATGATTGAT‑CCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCa < 2:1209539/139‑1 (MQ=255) cGCCATATTTATTGATGATTGAT‑CCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc < 2:1193608/103‑1 (MQ=255) cGCCATATTTATTGATGATTGAT‑CCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc > 1:1193608/1‑103 (MQ=255) cATATTTATTGATGATTGAT‑CCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTc > 2:1012501/1‑98 (MQ=255) cATATTTATTGATGATTGAT‑CCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTc < 1:1012501/98‑1 (MQ=255) ttGATGATTGAT‑CCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAg < 2:148379/53‑1 (MQ=255) ttGATGATTGAT‑CCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAg > 1:148379/1‑53 (MQ=255) tgatgaTTGAT‑CCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCa > 1:804347/1‑116 (MQ=255) tgatgaTTGAT‑CCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCa < 2:804347/116‑1 (MQ=255) gatgatTGAT‑CCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAAt > 2:1099757/1‑55 (MQ=255) gatgatTGAT‑CCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAAt < 1:1099757/55‑1 (MQ=255) atgatTGAT‑CCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGtgctgc < 1:579207/139‑1 (MQ=255) attgat‑CGCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTGGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTAc > 1:797575/1‑139 (MQ=255) attgat‑CCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTAc > 2:482331/1‑139 (MQ=255) at‑CCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACg > 1:744849/1‑96 (MQ=255) at‑CCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACg < 2:744849/96‑1 (MQ=255) at‑CCCCCGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGa > 2:250682/1‑139 (MQ=255) ccccGGT‑GGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGaaaa > 1:1217918/1‑139 (MQ=255) | ATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGAT‑CCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAA > NC_000913/321422‑321690 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |