Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,742,599 Δ1 bp intergenic (‑67/‑134) yiaJ ← / → yiaK transcriptional repressor for the yiaKLMNO‑lyxK‑sgbHUE operon/2,3‑diketo‑L‑gulonate reductase, NADH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,742,5980C.100.0% 62.0 / NA 16intergenic (‑66/‑135)yiaJ/yiaKtranscriptional repressor for the yiaKLMNO‑lyxK‑sgbHUE operon/2,3‑diketo‑L‑gulonate reductase, NADH‑dependent
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (7/9);  total (7/9)

GGCTTCCGGCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCAGATTGCCTTGT  >  NC_000913/3742463‑3742645
                                                                                                                                       |                                               
ggCTTCCGGCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAAcaat                                              >  1:273757/1‑136 (MQ=255)
ggCTTCCGGCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCAATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGCTAATTTATAAcaat                                              >  2:799565/1‑136 (MQ=255)
 gCTTCCGGCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAAcaat                                              >  1:759656/1‑135 (MQ=255)
 gCTTCCGGCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAAcaat                                              <  2:759656/138‑4 (MQ=255)
           ggACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATg                                  >  1:816353/1‑139 (MQ=255)
                          tGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATtcat                   <  2:83497/139‑1 (MQ=255)
                           gcgcCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAAcaat                                              <  1:1136002/112‑4 (MQ=255)
                           gcgcCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAAcaat                                              >  2:1136002/1‑109 (MQ=255)
                                 tctcGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATTCatcaatcct            <  2:62216/139‑3 (MQ=255)
                                 tctcGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATTCatcaatcct            >  2:1062534/1‑137 (MQ=255)
                                   tcGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATTCatcaatcctgt          <  2:816353/139‑5 (MQ=255)
                                    cGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATTCatcaatcctgtt         <  2:1098101/139‑6 (MQ=255)
                                    cGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATTCatcaatcctgtt         <  1:1062534/139‑6 (MQ=255)
                                    cGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATTCatcaatcctgtt         <  1:765274/139‑6 (MQ=255)
                                        ctctTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATTCatcaatcctgttgaca     >  2:806656/1‑130 (MQ=255)
                                           tttttttCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATTCatcaatcctgttgacaatt  >  1:427514/1‑127 (MQ=255)
                                                                               tCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATtcatca                 >  2:2585/1‑88 (MQ=255)
                                                                               tCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATtcatca                 <  1:2585/88‑1 (MQ=255)
                                                                                              aaaGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATTCatcaatcctgtt         <  2:7636/81‑6 (MQ=255)
                                                                                              aaaGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATTCatcaatcctgtt         >  1:7636/1‑76 (MQ=255)
                                                                                                                        aCAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATTCatcaatcctgtt         >  1:941983/1‑50 (MQ=255)
                                                                                                                        aCAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATTCatcaatcctgtt         <  2:941983/55‑6 (MQ=255)
                                                                                                                                       |                                               
GGCTTCCGGCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCAGATTGCCTTGT  >  NC_000913/3742463‑3742645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: