Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,920,205	(T)7→8	intergenic (+62/‑18)	yebT → / → rsmF	MCE domain protein/16S rRNA m(5)C1407 methyltransferase, SAM‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,920,198	1	.	T	100.0%	101.4 / NA	32	intergenic (+55/‑25)	yebT/rsmF	MCE domain protein/16S rRNA m(5)C1407 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (17/15);  total (17/15)

CCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑TTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTT‑CGACGCTCTCATTTGATGATTTTCTTGCCGCCTGTCAGCGCC  >  NC_000913/1920083‑1920328                                                                                                                     |                                                                                                                                    ccTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑ttttttttgc                                                                                                                           <  1:1011453/126‑4 (MQ=255) ccTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑ttttttttgc                                                                                                                           >  2:1011453/1‑123 (MQ=255)    gTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATTCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCg                                                                                                            <  2:795203/139‑1 (MQ=255)                       aaaGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTa                                                                                         >  1:182466/1‑139 (MQ=255)                                         ggAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCg                                                                                                            <  2:1096200/102‑1 (MQ=255)                                         ggAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCg                                                                                                            >  1:1096200/1‑102 (MQ=255)                                              tGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAg                                                                                                                         >  1:251197/1‑84 (MQ=255)                                              tGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAg                                                                                                                         <  2:251197/84‑1 (MQ=255)                                                            aatGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCg                                                    <  2:73270/139‑1 (MQ=255)                                                             atGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑ttttttttgc                                                                                                                           >  1:516395/1‑63 (MQ=255)                                                             atGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑ttttttttgc                                                                                                                           <  2:516395/66‑4 (MQ=255)                                                               gCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAg                                                                                                                         <  1:513733/67‑1 (MQ=255)                                                               gCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAg                                                                                                                         >  2:513733/1‑67 (MQ=255)                                                                cccACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGc                                                >  1:523652/1‑139 (MQ=255)                                                                   aCTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTg                                                                                                          <  2:920319/78‑1 (MQ=255)                                                                   aCTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTg                                                                                                          >  1:920319/1‑78 (MQ=255)                                                                     tGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTT‑Cg                                          >  2:473887/1‑139 (MQ=255)                                                                      gCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑ttttttttgcca                                                                                                                         >  1:187260/1‑54 (MQ=255)                                                                      gCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑ttttttttgcca                                                                                                                         <  2:187260/59‑6 (MQ=255)                                                                        tCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGAc                                                                               >  2:927260/1‑100 (MQ=255)                                                                        tCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGAc                                                                               <  1:927260/100‑1 (MQ=255)                                                                        tCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTT‑CGACg                                       <  2:182466/139‑1 (MQ=255)                                                                        tCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTT‑CGACg                                       <  2:709376/139‑1 (MQ=255)                                                                               tGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTc                                                                        >  2:472365/1‑100 (MQ=255)                                                                               tGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTc                                                                        <  1:472365/100‑1 (MQ=255)                                                                                 ccTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTccc                                                                                   >  1:361133/1‑87 (MQ=255)                                                                                 ccTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTccc                                                                                   <  2:361133/87‑1 (MQ=255)                                                                                 ccTGCGCCGGAGCGTTT‑TGCTAAACTGCGCGCCTGTTTTTTTTGGCAGTGGTAAATGCTTGTGGCCCAACCCTCCGTTTTTTTTCCGGGAGCCTTTTTGACACAAATTGGGCAAGGGGTGCCTTCCGAGGC‑CTCATTtg                             >  1:1079591/1‑139 (MQ=255)                                                                                          gAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTT‑CGACGCTCTCATTTGATGAtttt                     >  1:812684/1‑139 (MQ=255)                                                                                           aGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAg                                                                                                                         >  2:72031/1‑39 (MQ=255)                                                                                           aGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAg                                                                                                                         <  1:72031/39‑1 (MQ=255)                                                                                           aGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTT‑CGACGCTCTCATTTGATGATTTTc                    >  1:65181/1‑139 (MQ=255)                                                                                               tttATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGa                                                   >  1:553164/1‑105 (MQ=255)                                                                                               tttATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGa                                                   <  2:553164/105‑1 (MQ=255)                                                                                                          cTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTT‑CGACGCTCTCATTTGATGATTTTCTTGCCGCCTGTCAgc     <  2:812684/139‑1 (MQ=255)                                                                                                             cgcgcCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCtt                                             <  2:530570/97‑1 (MQ=255)                                                                                                             cgcgcCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCtt                                             >  1:530570/1‑97 (MQ=255)                                                                                                             cgcgcCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTT‑CGACGCTCTCATTTGATGATTTTCTTGCCGCCTGTCAGCGcc  >  2:110072/1‑139 (MQ=255)                                                                                                                     |                                                                                                                                    CCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑TTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTT‑CGACGCTCTCATTTGATGATTTTCTTGCCGCCTGTCAGCGCC  >  NC_000913/1920083‑1920328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑