Predicted mutation | ||||||
---|---|---|---|---|---|---|
evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 2,767,445 | (C)7→8 | intergenic (+90/‑265) | rnlB → / → yfjP | CP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, 50S ribosome‑binding GTPase family protein |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,767,438 | 1 | . | C | 93.0% | 130.2 / 2.6 | 43 | intergenic (+83/‑272) | rnlB/yfjP | CP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, 50S ribosome‑binding GTPase family protein |
Reads supporting (aligned to +/- strand): ref base . (3/0); new base C (20/20); total (23/20) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.36e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAA‑C‑CC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGG > NC_000913/2767305‑2767571 | tctGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAA‑c‑cc‑c > 1:736617/1‑138 (MQ=255) tctGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAA‑c‑cc‑c < 2:736617/138‑1 (MQ=255) ctGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAA‑c‑cc‑ccc > 1:792749/1‑139 (MQ=255) tGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAA‑c‑cc‑cccc > 1:1045063/1‑139 (MQ=255) gTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACC‑CC‑CCCCTcaca < 2:792749/139‑1 (MQ=255) gTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACC‑CC‑CCCCTcaca < 2:1045063/139‑1 (MQ=255) gCCAAGTCCTGTCAATGGATTTTTGAATCTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACC‑CC‑CCCCTCACAACGTCCTGCATAGGGa < 2:730640/138‑1 (MQ=255) gCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCTAATAGCATTAACC‑CC‑CCCCTCACAACGTCCTGCATAGGGa > 1:730640/1‑138 (MQ=255) gCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAA‑c‑cc‑c < 2:952872/113‑1 (MQ=255) gCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAA‑c‑cc‑c > 1:952872/1‑113 (MQ=255) ccAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACC‑CC‑CCCCTCACAACGTCCTGCATAGGGAac < 2:1117868/139‑1 (MQ=255) ccAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACC‑CC‑CCCCTCACAACGTCCTGCATAGGGAac < 1:463971/139‑1 (MQ=255) aGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACC‑CC‑CCCCTCACAACGTCCTGCATAGGGAACACg > 1:689690/1‑139 (MQ=255) gTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAA‑C‑CCTCCCCTCAAAAAGTACAGTATAGGGAACACga > 1:251424/1‑138 (MQ=255) ccTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACC‑CC‑CCCCTcaca < 1:455270/115‑1 (MQ=255) ccTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACC‑CC‑CCCCTcaca > 2:455270/1‑115 (MQ=255) ccTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACC‑CC‑CCCCTCACAACGTCCTGCATAGGGAACACGttt > 1:810387/1‑139 (MQ=255) tCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACC‑CC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTccc < 1:325329/139‑1 (MQ=255) tCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACC‑CC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTccc < 1:1119537/139‑1 (MQ=255) cAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACC‑CC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTcccc < 2:689690/139‑1 (MQ=255) ttttGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACC‑CC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCAcc < 2:810387/139‑1 (MQ=255) gtataagagactggTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACC‑CC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTccc < 2:598209/126‑1 (MQ=255) ggTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACC‑CC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACt < 2:558477/138‑1 (MQ=255) taGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACC‑CC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTccc > 1:44848/1‑98 (MQ=255) taGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACC‑CC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTccc < 2:44848/98‑1 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACC‑CC‑CCCCTcaca < 1:335944/62‑1 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACC‑CC‑CCCCTcaca > 2:335944/1‑62 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACC‑CC‑CCCCTCACAACGTCGTGGATAGGGAACACGTTTTCCCCTGTGGACCCACCACTAAATTCCCCCCCCGTTACATTTCTCAATAATgg > 2:772515/1‑138 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACC‑CC‑CCCCTCACAACGTCCTGCATCGGGTACACGATTCCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGt > 1:358663/1‑139 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAA‑CTCC‑CCCATCACAAAGTCCTGAATAAGGAACCCCTTTACCCCTGTGAAACCCCGACTAAAATTCACACCCTATAATCACCTCAATAATgc > 1:558477/1‑138 (MQ=255) atatTAACTTGTCCAATATGATCAAATAGCATTAACC‑CC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTgg < 2:782819/139‑1 (MQ=255) aaCTTGTCCAATATGATCAAATAGCATTAA‑C‑CC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCTTTGAAACTTCTATATAATTACACCAATTTTCTGGAACTcc > 1:767788/1‑139 (MQ=255) cTTGTCCAATATGATCAAATAGCATTAACC‑CC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCACTTTTCTGAAAATcca > 1:311759/1‑138 (MQ=255) gTCCAATATGATCAAATAGCATTAACC‑CC‑CCCCTCACAACGTCCTGCATAGGGAac < 2:336241/56‑1 (MQ=255) gTCCAATATGATCAAATAGCATTAACC‑CC‑CCCCTCACAACGTCCTGCATAGGGAac > 1:336241/1‑56 (MQ=255) gTCCAATATGATCAAATAGCATTAACC‑CC‑CCCCTCACAACGTCCTACATAGGGAACACGTTTTCCCCTCTGCACCCACGACTAAATTTCACCACCTTTAAACTTCTCAATAATGTCACCACTTTTCTGGAAATCCTacg > 2:21188/1‑137 (MQ=255) tCCAATATGATCAAATAGCATTAACC‑CC‑CCCCTCACAACGTCCTGCATAGGGAACACGtt > 2:14283/1‑60 (MQ=255) tCCAATATGATCAAATAGCATTAACC‑CC‑CCCCTCACAACGTCCTGCATAGGGAACACGtt < 1:14283/60‑1 (MQ=255) tCCAATATGATCAAATAGCATTAACC‑CC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATTTCACCAATTTTCTGGAAATCCTatga > 1:656934/1‑139 (MQ=255) tatGATCAAATAGCATTAACC‑CC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCAcc < 1:1072110/69‑1 (MQ=255) tatGATCAAATAGCATTAACC‑CC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCAcc > 2:1072110/1‑69 (MQ=255) tCAAATAGCATTAACC‑CC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTCAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTccc > 1:668413/1‑139 (MQ=255) tCAAATAGCATTAACC‑CC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTCCCCCTGTGCACCCACGACTAAATTTCTCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAACTCCTATGTTGCCTTTccc > 1:548580/1‑139 (MQ=255) tCAAATAGCATTAACC‑CC‑CCCCTCACAACGTCCTGCATAGGGAACAAGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCAACAATTTTCTGGAAATCCTATGATGCCTTTccc > 1:372567/1‑139 (MQ=255) cATTAACC‑CC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCAcc < 2:632291/56‑1 (MQ=255) cATTAACC‑CC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCAcc > 1:632291/1‑56 (MQ=255) aTTAACC‑CC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACtt < 2:179915/84‑1 (MQ=255) aTTAACC‑CC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCACCCTTTAAACtt > 1:179915/1‑84 (MQ=255) aTTAACC‑CC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTGCTCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAAAAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATgg > 1:314711/1‑139 (MQ=255) | TCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAA‑C‑CC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGG > NC_000913/2767305‑2767571 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |