Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,767,445 (C)7→8 intergenic (+90/‑265) rnlB → / → yfjP CP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, 50S ribosome‑binding GTPase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,767,4381.C93.0% 130.2 / 2.6 43intergenic (+83/‑272)rnlB/yfjPCP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, 50S ribosome‑binding GTPase family protein
Reads supporting (aligned to +/- strand):  ref base . (3/0);  new base C (20/20);  total (23/20)
Fisher's exact test for biased strand distribution p-value = 2.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGG  >  NC_000913/2767305‑2767571
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tctGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAAcccc                                                                                                                                   >  1:736617/1‑138 (MQ=255)
tctGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAAcccc                                                                                                                                   <  2:736617/138‑1 (MQ=255)
 ctGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAAcccccc                                                                                                                                 >  1:792749/1‑139 (MQ=255)
  tGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAAccccccc                                                                                                                                >  1:1045063/1‑139 (MQ=255)
        gTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTcaca                                                                                                                           <  2:792749/139‑1 (MQ=255)
        gTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTcaca                                                                                                                           <  2:1045063/139‑1 (MQ=255)
                         gCCAAGTCCTGTCAATGGATTTTTGAATCTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGa                                                                                                           <  2:730640/138‑1 (MQ=255)
                         gCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCTAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGa                                                                                                           >  1:730640/1‑138 (MQ=255)
                         gCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAAcccc                                                                                                                                   <  2:952872/113‑1 (MQ=255)
                         gCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAAcccc                                                                                                                                   >  1:952872/1‑113 (MQ=255)
                          ccAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAac                                                                                                         <  2:1117868/139‑1 (MQ=255)
                          ccAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAac                                                                                                         <  1:463971/139‑1 (MQ=255)
                             aGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACg                                                                                                      >  1:689690/1‑139 (MQ=255)
                              gTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCTCCCCTCAAAAAGTACAGTATAGGGAACACga                                                                                                     >  1:251424/1‑138 (MQ=255)
                                ccTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTcaca                                                                                                                           <  1:455270/115‑1 (MQ=255)
                                ccTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTcaca                                                                                                                           >  2:455270/1‑115 (MQ=255)
                                ccTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGttt                                                                                                   >  1:810387/1‑139 (MQ=255)
                                    tCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTccc                                                                                               <  1:325329/139‑1 (MQ=255)
                                    tCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTccc                                                                                               <  1:1119537/139‑1 (MQ=255)
                                     cAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTcccc                                                                                              <  2:689690/139‑1 (MQ=255)
                                             ttttGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCAcc                                                                                      <  2:810387/139‑1 (MQ=255)
                                                             gtataagagactggTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTccc                                                                      <  2:598209/126‑1 (MQ=255)
                                                                         ggTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACt                                                          <  2:558477/138‑1 (MQ=255)
                                                                             taGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTccc                                                                                               >  1:44848/1‑98 (MQ=255)
                                                                             taGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTccc                                                                                               <  2:44848/98‑1 (MQ=255)
                                                                                     gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTcaca                                                                                                                           <  1:335944/62‑1 (MQ=255)
                                                                                     gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTcaca                                                                                                                           >  2:335944/1‑62 (MQ=255)
                                                                                     gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCGTGGATAGGGAACACGTTTTCCCCTGTGGACCCACCACTAAATTCCCCCCCCGTTACATTTCTCAATAATgg                                              >  2:772515/1‑138 (MQ=255)
                                                                                     gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATCGGGTACACGATTCCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGt                                              >  1:358663/1‑139 (MQ=255)
                                                                                     gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACTCCCCCATCACAAAGTCCTGAATAAGGAACCCCTTTACCCCTGTGAAACCCCGACTAAAATTCACACCCTATAATCACCTCAATAATgc                                              >  1:558477/1‑138 (MQ=255)
                                                                                                   atatTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTgg                                <  2:782819/139‑1 (MQ=255)
                                                                                                        aaCTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCTTTGAAACTTCTATATAATTACACCAATTTTCTGGAACTcc                          >  1:767788/1‑139 (MQ=255)
                                                                                                          cTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCACTTTTCTGAAAATcca                         >  1:311759/1‑138 (MQ=255)
                                                                                                             gTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAac                                                                                                         <  2:336241/56‑1 (MQ=255)
                                                                                                             gTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAac                                                                                                         >  1:336241/1‑56 (MQ=255)
                                                                                                             gTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTACATAGGGAACACGTTTTCCCCTCTGCACCCACGACTAAATTTCACCACCTTTAAACTTCTCAATAATGTCACCACTTTTCTGGAAATCCTacg                      >  2:21188/1‑137 (MQ=255)
                                                                                                              tCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGtt                                                                                                    >  2:14283/1‑60 (MQ=255)
                                                                                                              tCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGtt                                                                                                    <  1:14283/60‑1 (MQ=255)
                                                                                                              tCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATTTCACCAATTTTCTGGAAATCCTatga                     >  1:656934/1‑139 (MQ=255)
                                                                                                                   tatGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCAcc                                                                                      <  1:1072110/69‑1 (MQ=255)
                                                                                                                   tatGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCAcc                                                                                      >  2:1072110/1‑69 (MQ=255)
                                                                                                                        tCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTCAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTccc           >  1:668413/1‑139 (MQ=255)
                                                                                                                        tCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTCCCCCTGTGCACCCACGACTAAATTTCTCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAACTCCTATGTTGCCTTTccc           >  1:548580/1‑139 (MQ=255)
                                                                                                                        tCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACAAGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCAACAATTTTCTGGAAATCCTATGATGCCTTTccc           >  1:372567/1‑139 (MQ=255)
                                                                                                                                cATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCAcc                                                                                      <  2:632291/56‑1 (MQ=255)
                                                                                                                                cATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCAcc                                                                                      >  1:632291/1‑56 (MQ=255)
                                                                                                                                 aTTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACtt                                                         <  2:179915/84‑1 (MQ=255)
                                                                                                                                 aTTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCACCCTTTAAACtt                                                         >  1:179915/1‑84 (MQ=255)
                                                                                                                                 aTTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTGCTCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAAAAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATgg  >  1:314711/1‑139 (MQ=255)
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TCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGG  >  NC_000913/2767305‑2767571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: