Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 2,614,614 Δ3 bp coding (681‑683/849 nt) focB → putative formate transporter

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 2614614 2614616 3 33 [0] [0] 31 focB putative formate transporter

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 26146130 (0.000)31 (0.600) 28/214 0.7 100% coding (680/849 nt) focB putative formate transporter
?NC_000913 2614617 = 0 (0.000)coding (684/849 nt) focB putative formate transporter

TGCGGGCGTTCGTTATGCGACAAAATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2614480‑2614613
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ccCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGGCGGTGCGGTGCTGGTGAGTATGTGTTAT  >  NC_000913/2614617‑2614752
                                                                                                                                                                                                                                                                              
TGCGGGCGTTCGTTATGCGACAAAATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCT                                                                                                                                     >  2:936400/1‑139
      CGTTCGTTATGCGACAAAATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGC                                                                                                                               >  1:1015475/1‑139
        TTCGTTAGGCGACAAAATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTCTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTACGCCCCCCCCTTCTGGCAG                                                                                                                             >  1:795503/1‑139
         TCGTTATGCGACAAAATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGC                                                                                                                            <  2:1015475/139‑1
                    CAAAATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGT                                                                                                                 >  1:1197844/1‑139
                    CAAAATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCG                                                                                                                       >  1:277170/1‑133
                    CAAAATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCG                                                                                                                       <  2:277170/133‑1
                          CGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCA                                                                                                           <  2:1197844/139‑1
                             CGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGAC                                                                                                        <  2:543886/139‑1
                                        TGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGC                                                                                             >  1:922281/1‑139
                                          CCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCG                                                                                                                       >  1:1049420/1‑111
                                          CCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCG                                                                                                                       <  2:1049420/111‑1
                                                 CCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGT                                                                                    <  1:130799/139‑1
                                                           CGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTT                                                                          >  2:1173287/1‑139
                                                              CAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCC                                                                                                                                          >  1:180881/1‑72
                                                              CAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCC                                                                                                                                          <  2:180881/72‑1
                                                                GTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGT                                                                                    >  1:90856/1‑124
                                                                GTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGT                                                                                    <  2:90856/124‑1
                                                                            ACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCT                                                         <  1:936400/139‑1
                                                                             CTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTC                                                        <  2:922281/139‑1
                                                                               GTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCC                                                      <  2:795503/139‑1
                                                                                          TTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGG                                           <  1:234592/139‑1
                                                                                             TTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTA                                        <  1:1173287/139‑1
                                                                                                   ATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGG                                                                                              >  2:40068/1‑79
                                                                                                   ATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGG                                                                                              <  1:40068/79‑1
                                                                                                          TCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGGCGG                           >  2:294444/1‑139
                                                                                                            GCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGGCGGTG                         <  2:1106052/139‑1
                                                                                                                 TGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCA                                                                                  <  2:39928/77‑1
                                                                                                                 TGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCA                                                                                  >  1:39928/1‑77
                                                                                                                  GCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGGCGGTGCGGTGC                   >  1:1081368/1‑139
                                                                                                                     ATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGGCGGTGCGGTGCTGG                <  2:513379/139‑1
                                                                                                                        CGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGGCGGTGCGGTGCTGGTGA             <  1:294444/139‑1
                                                                                                                                   CCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGGCGGTGCGGTGCTGGTGAGTATGTGTTAT  <  2:273312/139‑1
                                                                                                                                                                                                                                                                              
TGCGGGCGTTCGTTATGCGACAAAATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2614480‑2614613
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ccCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGGCGGTGCGGTGCTGGTGAGTATGTGTTAT  >  NC_000913/2614617‑2614752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.