Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	499,014	C→T	R320R (CGC→CGT)  *320* (TAG→TAA)	hemH → aes ←	ferrochelatase acetyl esterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	499,014	0	C	T	100.0%	128.7 / NA	41	R320R (CGC→CGT)  *320* (TAG→TAA)	hemH aes	ferrochelatase acetyl esterase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/20);  total (21/20)

CTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTCACAGGGCTGCTGATGCGCCGCT  >  NC_000913/498883‑499149                                                                                                                                    |                                                                                                                                        cTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCt                                                                                                                                  >  2:1031952/1‑139 (MQ=255) cTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCt                                                                                                                                  >  1:638083/1‑139 (MQ=255) cTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCt                                                                                                                                  >  2:183971/1‑139 (MQ=255) cTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCt                                                                                                                                  >  2:259218/1‑139 (MQ=255)    gAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAg                                                                                                                               <  1:259218/139‑1 (MQ=255)            tGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTg                                                                                                                                 <  1:822742/129‑1 (MQ=255)            tGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAAGATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTCCCGCGTATCGTTAAAGCTg                                                                                                                                 >  2:822742/1‑129 (MQ=255)                                         gTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCtcgtc                                                                                          >  1:983499/1‑139 (MQ=255)                                          tGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCtcgtcg                                                                                         >  2:716681/1‑139 (MQ=255)                                           gCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTcgg                                                                                        <  1:716681/139‑1 (MQ=255)                                                    aaaaaaTATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAg                                                                                                                               <  2:79754/91‑1 (MQ=255)                                                    aaaaaaTATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAg                                                                                                                               >  1:79754/1‑91 (MQ=255)                                                    aaaaaaTATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTtca                                                                               <  1:40396/139‑1 (MQ=255)                                                     aaaaaTATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTtcat                                                                              <  1:139875/139‑1 (MQ=255)                                                     aaaaaTATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTtcat                                                                              <  1:183971/139‑1 (MQ=255)                                                     aaaaaTATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTtcat                                                                              <  1:1031952/139‑1 (MQ=255)                                                          tatGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATcc                                                                         <  1:696612/139‑1 (MQ=255)                                                                   atTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCCTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGaataat                                                                >  2:192474/1‑139 (MQ=255)                                                                    ttCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTcgg                                                                                        <  2:114377/114‑1 (MQ=255)                                                                    ttCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTcgg                                                                                        >  1:114377/1‑114 (MQ=255)                                                                         gcgcTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAg                                                                                                                               >  1:208510/1‑70 (MQ=255)                                                                         gcgcTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAg                                                                                                                               <  2:208510/70‑1 (MQ=255)                                                                          cgcTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTtcat                                                                              >  1:1061743/1‑118 (MQ=255)                                                                          cgcTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTtcat                                                                              <  2:1061743/118‑1 (MQ=255)                                                                             ttAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGc                                                      >  2:1086936/1‑139 (MQ=255)                                                                                       gCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTg                                            >  1:764209/1‑139 (MQ=255)                                                                                                           tgGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAg                                                                                                                               <  1:1008945/36‑1 (MQ=255)                                                                                                           tgGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAg                                                                                                                               >  2:1008945/1‑36 (MQ=255)                                                                                                            ggCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGaa                                                                    <  2:314892/94‑1 (MQ=255)                                                                                                            ggCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGaa                                                                    >  1:314892/1‑94 (MQ=255)                                                                                                            ggCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTca                       >  1:1009007/1‑139 (MQ=255)                                                                                                            ggCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTca                       >  1:399821/1‑139 (MQ=255)                                                                                                                 aTCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTCACAggg                  >  2:942636/1‑139 (MQ=255)                                                                                                                  tCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTca                       >  1:978832/1‑133 (MQ=255)                                                                                                                  tCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTca                       <  2:978832/133‑1 (MQ=255)                                                                                                                            gcgTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTCACAGGGCTGCTGATgcg       <  2:1009007/139‑1 (MQ=255)                                                                                                                             cgTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGaata                                                                  <  2:19112/79‑1 (MQ=255)                                                                                                                             cgTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGaata                                                                  >  1:19112/1‑79 (MQ=255)                                                                                                                                aTCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGTAGCGTGCCTGGGTAGAGTTTGAACTCACAGGGCTGCTGATGcgccgc   <  1:64247/139‑1 (MQ=255)                                                                                                                                aTCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGTAGCGTGCCTGGGTAGAGTTTGAACTCACAGGGCTGCTGATGcgccgc   <  1:355710/139‑1 (MQ=255)                                                                                                                                 tCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTCACAGGGCTGCTGATGCGCCGCt  <  2:399821/139‑1 (MQ=255)                                                                                                                                    |                                                                                                                                        CTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTCACAGGGCTGCTGATGCGCCGCT  >  NC_000913/498883‑499149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑