| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 551,137 | G→T | T233T (ACG→ACT) | ybcF → | putative carbonate kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 551,137 | 0 | G | T | 98.1% | 163.4 / NA | 53 | T233T (ACG→ACT) | ybcF | putative carbonate kinase |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (26/26); total (27/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
ACGGATGACGGAGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGCGCCATTTGCCAAAGCCGATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGAT > NC_000913/551006‑551268 | acggatgacggaGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACTCCgcagc > 1:1081995/1‑139 (MQ=255) atgacggaGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACTCCGCAGCAACg > 1:748678/1‑139 (MQ=255) atgacggaGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACTCCGCAGCAACg > 2:1090813/1‑139 (MQ=255) tgacggaGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACTCCGCAGCAACGt > 1:1009515/1‑139 (MQ=255) ggaGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACTCCGCAGCAACGTGCCa < 1:1090813/139‑1 (MQ=255) cAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACTCCGCAGCACCGTGccttacg < 1:708102/139‑6 (MQ=255) cAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACTCCGCAGCAACGTGCCATTCg < 1:845989/139‑1 (MQ=255) gAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACTCCGCAGCAACGTGCCATTCGCCATGCca < 1:1073359/139‑1 (MQ=255) gTGATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACTCCGCAGCAACGTGCCATTCGCCATGCCACACCgg < 1:976141/139‑1 (MQ=255) gatAAAGATGTCGCCGCTTCGGTGCTCCCCGAGTAGATGAATGTTGATCGACTGTGGAGCCTCACCGGTGCTGATGCGGTATATGCAAACTGGGGAACTCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGt > 1:488042/1‑139 (MQ=255) aGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACTCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAgcg > 1:524173/1‑139 (MQ=255) aTCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACTCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGCGcc < 2:748678/139‑1 (MQ=255) ctcGCCGCTGTGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACTCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATg < 2:760460/127‑1 (MQ=255) ctcGCCGCTGTGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACTCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATg > 1:760460/1‑127 (MQ=255) gccgcTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACTCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGCGCCATTTg > 1:983239/1‑139 (MQ=255) gccgcTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACTCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGCGCCATTTg > 1:246651/1‑139 (MQ=255) gccgcTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACTCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGCGCCATTTg > 1:618388/1‑139 (MQ=255) tGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACTCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGCGCCATTTGCCaaa > 2:898625/1‑139 (MQ=255) tGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACTCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGCGCCATTTGCCaaa > 1:19553/1‑139 (MQ=255) gTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACTCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGCGCCATTTGCCAAAGcc < 1:898625/139‑1 (MQ=255) gCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACTCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGCGCCATTTGCCAAAGCCGATGGtt < 2:19553/139‑1 (MQ=255) aGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACTCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGCGCCATTTGCCAAAGCCGATGGTTCGAt < 2:1009515/139‑1 (MQ=255) aTGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACTCCGCAGCAACGTGCCATTCg > 2:560106/1‑73 (MQ=255) aTGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACTCCGCAGCAACGTGCCATTCg < 1:560106/73‑1 (MQ=255) aTGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACTCCGCAGCAACGTGCCATTCg < 2:453401/73‑1 (MQ=255) aTGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACTCCGCAGCAACGTGCCATTCg > 1:453401/1‑73 (MQ=255) aTGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACTCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGCGCCATTTGCCAAAGCCGATGGTTCGATGGGGCCGAATGTAAc > 2:335707/1‑139 (MQ=255) aCTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACTCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGCGCCATTTGCCAAAGCCGATGGTTCGATGGGGCCGAATGTAAcggcg < 2:618388/139‑1 (MQ=255) cTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACTCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGCGCCATTTGCCaa > 1:592637/1‑104 (MQ=255) cTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACTCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGCGCCATTTGCCaa < 2:592637/104‑1 (MQ=255) gTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACTCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGCGCCATTTGCCAAAGCCGATGGTTCGATGGGGCCGAATGTAACGGCGGTGa < 1:335707/139‑1 (MQ=255) tCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACTCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGCGCCATTTGCCAAAGCCGATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTgg > 1:374110/1‑139 (MQ=255) ccTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACTCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGCGCCATTTGCCAAAGCCGATGGTTCGATGGGGCCGAATGTAAcg < 2:807162/127‑1 (MQ=255) ccTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACTCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGCGCCATTTGCCAAAGCCGATGGTTCGATGGGGCCGAATGTAAcg > 1:807162/1‑127 (MQ=255) cTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACTCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATg < 1:991756/76‑1 (MQ=255) cTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACTCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATg > 2:991756/1‑76 (MQ=255) cTCACCGATGCTGATGCGGTACATGAAAACTGGGGAACTCCGCAGCAACGTGCCATTCGCCATGCCACACCCGATGAGTTAGCGCCATTTGCCAAAGCCGATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGtt < 1:710702/139‑1 (MQ=255) cGATGCTGATGCGGTATATGAAAACTGGGGAACTCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGCGCCATTTGCCAAAGCCGATGGTTCGATGGGGCCGAATGTAACGGCGGTGa > 1:38606/1‑128 (MQ=255) cGATGCTGATGCGGTATATGAAAACTGGGGAACTCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGCGCCATTTGCCAAAGCCGATGGTTCGATGGGGCCGAATGTAACGGCGGTGa < 2:38606/128‑1 (MQ=255) aTGCTGATGCGGTATATGAAAACTGGGGAACTCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGCGCCATTTGCCAAAGCCGATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAg > 1:24004/1‑139 (MQ=255) gCTGATGCGGTATATGAAAACTGGGGAACTCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGCGCCATTTGCCAAAGCCGATGGTTCGa > 1:780820/1‑100 (MQ=255) gCTGATGCGGTATATGAAAACTGGGGAACTCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGCGCCATTTGCCAAAGCCGATGGTTCGa < 2:780820/100‑1 (MQ=255) tGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGCGCCATTTGCCAAAGCCGATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTGATGTCagcagc > 2:788559/1‑139 (MQ=255) gTATATGAAAACTGGGGAACTCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGCGCCATTTGCCAAAGCCGATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTa > 1:999234/1‑139 (MQ=255) atatGAAAACTGGGGAACTCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGCGCCATTTGCCAAAGCCGATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTaaa < 2:24004/139‑1 (MQ=255) aaaaCTGGGGAACTCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGCGCCATTTGCCAAAGCCGATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCcgc < 2:999234/139‑1 (MQ=255) aaaCTGGGGAACTCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGCGCCATTTGCCAAAGCCGATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCcgcg < 2:246651/139‑1 (MQ=255) cTGGGGAACTCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGCGCCAt > 1:342909/1‑59 (MQ=255) cTGGGGAACTCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGCGCCAt < 2:342909/59‑1 (MQ=255) ggggAACTCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGCGCCATTTGCCAAAGCCGATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGt < 1:853752/135‑1 (MQ=255) ggggAACTCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGCGCCATTTGCCAAAGCCGATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGt > 2:853752/1‑135 (MQ=255) ggggAACTCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGCGCCATTTGCCAAAGCCGATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGAt > 2:937782/1‑139 (MQ=255) aaCTCCGCAGCAACGTGCCATTCGCCATGCCacac < 1:434323/35‑1 (MQ=255) | ACGGATGACGGAGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGCGCCATTTGCCAAAGCCGATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGAT > NC_000913/551006‑551268 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |