Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 582,152 C→T pseudogene (653/653 nt) ybcY ← pseudogene, DLP12 prophage, methyltransferase homology,Phage or Prophage Related

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913582,1520CT100.0% 242.8 / NA 74pseudogene (653/653 nt)ybcYpseudogene, DLP12 prophage, methyltransferase homology,Phage or Prophage Related
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (38/36);  total (38/36)

GAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCTTTCTGAT  >  NC_000913/582034‑582285
                                                                                                                      |                                                                                                                                     
gAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGaa                                                                                                                  <  2:2911/139‑1 (MQ=255)
gAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGaa                                                                                                                  <  2:201826/139‑1 (MQ=255)
  aCCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGaaaa                                                                                                                <  1:935849/139‑1 (MQ=255)
  aCCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGaaaa                                                                                                                <  1:415421/139‑1 (MQ=255)
     gtgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                             <  2:607535/139‑1 (MQ=255)
      tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGaaaa                                                                                                                <  2:276031/135‑1 (MQ=255)
      tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGaaaa                                                                                                                >  1:276031/1‑135 (MQ=255)
      tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                            >  1:78610/1‑139 (MQ=255)
      tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                            >  1:550378/1‑139 (MQ=255)
             aCATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAg                                                                                                     <  2:121092/139‑1 (MQ=255)
             aCATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAg                                                                                                     <  1:569783/139‑1 (MQ=255)
               aTCAACTACACCTGATATTGAGTGCCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTa                                                                                                   >  2:743495/1‑139 (MQ=255)
                tCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTAc                                                                                                  >  1:713921/1‑139 (MQ=255)
                     tGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGa                                                                                             >  2:732371/1‑139 (MQ=255)
                            gATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTgg                                                                                      >  2:999348/1‑139 (MQ=255)
                                    gTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATtacta                                                                                                        <  2:190080/113‑1 (MQ=255)
                                    gTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATtacta                                                                                                        >  1:190080/1‑113 (MQ=255)
                                       gCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACa                                                                                                      <  2:812348/112‑1 (MQ=255)
                                       gCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACa                                                                                                      >  1:812348/1‑112 (MQ=255)
                                       gCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATtc                                                                           >  2:1067557/1‑139 (MQ=255)
                                        ccTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATtct                                                                          >  1:488463/1‑139 (MQ=255)
                                        ccTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATtct                                                                          >  1:632683/1‑139 (MQ=255)
                                           aCGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGa                                                                       <  2:281760/139‑1 (MQ=255)
                                            cGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGaa                                                                      >  2:220497/1‑139 (MQ=255)
                                            cGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGaa                                                                      <  2:78610/139‑1 (MQ=255)
                                             gAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTg                                                                                              <  1:528889/114‑1 (MQ=255)
                                             gAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTg                                                                                              >  2:528889/1‑114 (MQ=255)
                                                 ccTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGct                                                                 <  2:362206/139‑1 (MQ=255)
                                                 ccTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGct                                                                 >  1:1206169/1‑139 (MQ=255)
                                                 ccTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGct                                                                 <  1:999348/139‑1 (MQ=255)
                                                 ccTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGct                                                                 <  1:657580/139‑1 (MQ=255)
                                                 ccTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGct                                                                 <  1:357684/139‑1 (MQ=255)
                                                  cTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATtactac                                                                                                       <  2:45208/100‑1 (MQ=255)
                                                  cTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATtactac                                                                                                       >  1:45208/1‑100 (MQ=255)
                                                   tGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                               <  1:220497/139‑1 (MQ=255)
                                                   tGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                               <  2:93859/139‑1 (MQ=255)
                                                   tGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                               <  1:137387/139‑1 (MQ=255)
                                                   tGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                               <  2:1206169/139‑1 (MQ=255)
                                                     tCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTga                                                             <  1:194081/139‑1 (MQ=255)
                                                         ggAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGt                                                         >  2:948181/1‑139 (MQ=255)
                                                               aTCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATAtgt                                                   >  2:1006792/1‑139 (MQ=255)
                                                                     gggATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGATGCGGAAAACATtacta                                                                                                        >  1:910507/1‑80 (MQ=255)
                                                                     gggATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATtacta                                                                                                        <  2:910507/80‑1 (MQ=255)
                                                                         ttATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGa                                                                                                                   >  2:531402/1‑65 (MQ=255)
                                                                         ttATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGa                                                                                                                   <  1:531402/65‑1 (MQ=255)
                                                                          tATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGaa                                                                                                                  >  2:659971/1‑65 (MQ=255)
                                                                          tATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGaa                                                                                                                  <  1:659971/65‑1 (MQ=255)
                                                                          tATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                               >  2:1164446/1‑116 (MQ=255)
                                                                          tATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                               <  1:1164446/116‑1 (MQ=255)
                                                                           aTGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCcttct                                       >  1:918635/1‑139 (MQ=255)
                                                                            tGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATta                                                                                                           >  1:157342/1‑70 (MQ=255)
                                                                            tGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATta                                                                                                           <  2:157342/70‑1 (MQ=255)
                                                                                      cGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCt                                                                                         <  1:1155954/78‑1 (MQ=255)
                                                                                      cGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCt                                                                                         >  2:1155954/1‑78 (MQ=255)
                                                                                        tCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTtg                          <  1:948181/139‑1 (MQ=255)
                                                                                                 aGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGaaa                 <  2:713921/139‑1 (MQ=255)
                                                                                                   aaCATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTaa                                                                                >  1:756597/1‑74 (MQ=255)
                                                                                                   aaCATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTaa                                                                                <  2:756597/74‑1 (MQ=255)
                                                                                                   aaCATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCTGAATCTTTTGTGTTTGAAAAg               >  2:716537/1‑139 (MQ=255)
                                                                                                   aaCATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTtgt                         <  2:381951/129‑1 (MQ=255)
                                                                                                   aaCATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTtgt                         >  1:381951/1‑129 (MQ=255)
                                                                                                   aaCATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGaaaac               >  2:1169934/1‑138 (MQ=255)
                                                                                                   aaCATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAg               >  2:1153452/1‑139 (MQ=255)
                                                                                                   aaCATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAg               >  2:1070339/1‑139 (MQ=255)
                                                                                                   aaCATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAg               >  1:830251/1‑139 (MQ=255)
                                                                                                       tgctgcGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTaa                                                                                <  2:28363/70‑1 (MQ=255)
                                                                                                       tgctgcGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTaa                                                                                >  1:28363/1‑70 (MQ=255)
                                                                                                       tgctgcGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGc           >  2:173361/1‑139 (MQ=255)
                                                                                                           gcGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCttt       <  2:632683/139‑1 (MQ=255)
                                                                                                           gcGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCCTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTCTGTGTTTGAAAAGATGCCtat       <  1:732371/139‑3 (MQ=255)
                                                                                                            cGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCTTTc      >  2:674172/1‑139 (MQ=255)
                                                                                                              ggttgATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCTTTCt     >  2:947722/5‑139 (MQ=255)
                                                                                                                ttgATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCTTTCTGa   >  2:425565/3‑139 (MQ=255)
                                                                                                                 tgATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCTTTCTGAt  >  1:1059164/2‑139 (MQ=255)
                                                                                                                      |                                                                                                                                     
GAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCTTTCTGAT  >  NC_000913/582034‑582285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: