Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	610,746	C→T	A583A (GCG→GCA)	fepA ←	ferrienterobactin outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	610,746	0	C	T	100.0%	206.8 / NA	64	A583A (GCG→GCA)	fepA	ferrienterobactin outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/30);  total (34/30)

GGATGATCGACAAACGGTCGCCCGTGGTTTTGTTTTCACTCTTCAGCATATAAGTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACCGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTCGCG  >  NC_000913/610622‑610877                                                                                                                             |                                                                                                                                    ggATGATCGACAAACGGTCGCCCGTGGTTTTGTTTTCACTCTTCAGCATATAAGTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGtt                                                                                                                       <  1:651348/139‑1 (MQ=255)  gatgatCGACAAACGGTCGCCCGTGGTTTTGTTTTCACTCTTCAGCATATAAGTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTa                                                                                                                      <  1:218315/139‑1 (MQ=255)  gatgatCGACAAACGGTCGCCCGTGGTTTTGTTTTCACTCTTCAGCATATAAGTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTa                                                                                                                      >  2:218315/1‑139 (MQ=255)   atgatCGACAAACGGTCGCCCGTGGTTTTGTTTTCACTCTTCAGCATATAAGTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTAt                                                                                                                     <  1:1064413/139‑1 (MQ=255)   atgatCGACAAACGGTCGCCCGTGGTTTTGTTTTCACTCTTCAGCATATAAGTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTAt                                                                                                                     <  2:437165/139‑1 (MQ=255)   atgatCGACAAACGGTCGCCCGTGGTTTTGTTTTCACTCTTCAGCATATAAGTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTAt                                                                                                                     <  1:681432/139‑1 (MQ=255)     gatCGACAAACGGTCGCCCGTGGTTTTGTTTTCACTCTTCAGCATATAAGTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATcc                                                                                                                   <  2:343506/139‑1 (MQ=255)      atCGACAAACGGTCGCCCGTGGTTTTGTTTTCACTCTTCAGCATATAAGTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTAt                                                                                                                     >  1:973641/1‑136 (MQ=255)      atCGACAAACGGTCGCCCGTGGTTTTGTTTTCACTCTTCAGCATATAAGTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTAt                                                                                                                     <  2:973641/136‑1 (MQ=255)       tCGACAAACGGTCGCCCGTGGTTTTGTTTTCACTCTTCAGCATATAAGTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCa                                                                                                                 <  2:185739/139‑1 (MQ=255)                 gTCGCCCGTGGTTTTGTTTTCACTCTTCAGCATATAAGTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAgaga                                                                                                       >  1:417813/1‑139 (MQ=255)                  tCGCCCGTGGTTTTGTTTTCACTCTTCAGCATATAAGTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGAt                                                                                                      <  2:444527/139‑1 (MQ=255)                                        tctTCAGCATATAAGTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGccc                                                                                <  2:1133670/139‑1 (MQ=255)                                        tctTCAGCATATAAGTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGccc                                                                                <  1:495398/139‑1 (MQ=255)                                            ctgCATATATGTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTTCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACa                                                                            <  1:512105/137‑1 (MQ=255)                                            cAGCATATAAGTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCa                                                                                                                 >  2:362287/1‑102 (MQ=255)                                            cAGCATATAAGTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCa                                                                                                                 <  1:362287/102‑1 (MQ=255)                                             aGCATATAAGTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTAGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAg                                                                           >  2:806610/1‑139 (MQ=255)                                                  ataAGTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCTGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCcaca                                                                      <  1:806610/139‑1 (MQ=255)                                                  ataAGTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCAc                                                                                                                >  1:1006435/1‑97 (MQ=255)                                                  ataAGTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCAc                                                                                                                <  2:1006435/97‑1 (MQ=255)                                                  ataAGTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCcaca                                                                      >  2:575469/1‑139 (MQ=255)                                                      gTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGc                                                                  >  1:779186/1‑139 (MQ=255)                                                         aTGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTAt                                                                                                                     >  1:417175/1‑85 (MQ=255)                                                         aTGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTAt                                                                                                                     <  2:417175/85‑1 (MQ=255)                                                         aTGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCTGACTGCGTTTTGCCCTACAGCCACATAGCCCg                                                               >  1:769974/1‑139 (MQ=255)                                                         aTGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGCCCg                                                               >  2:753438/1‑139 (MQ=255)                                                              aTTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGCCCGCTTCa                                                          >  2:945582/1‑139 (MQ=255)                                                                   tCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGtt                                                                                      <  2:356393/106‑1 (MQ=255)                                                                   tCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGtt                                                                                      >  1:356393/1‑106 (MQ=255)                                                                      acaTCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAg                                                                           <  2:580370/114‑1 (MQ=255)                                                                      acaTCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAg                                                                           >  1:580370/1‑114 (MQ=255)                                                                       caTCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGc                                                                                                <  1:231435/92‑1 (MQ=255)                                                                       caTCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGc                                                                                                >  2:231435/1‑92 (MQ=255)                                                                        aTCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCa                                                                                                                           <  2:223990/64‑1 (MQ=255)                                                                        aTCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCa                                                                                                                           >  1:223990/1‑64 (MQ=255)                                                                        aTCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACg                                                                                                                         <  1:731597/66‑1 (MQ=255)                                                                        aTCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACg                                                                                                                         >  2:731597/1‑66 (MQ=255)                                                                        aTCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTa                                                                              >  2:268925/1‑109 (MQ=255)                                                                        aTCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTa                                                                              <  1:268925/109‑1 (MQ=255)                                                                          cACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATCTTATTGCGa                                              <  2:837179/139‑1 (MQ=255)                                                                            ccGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATCTTATTGCGATa                                            >  1:1117506/1‑139 (MQ=255)                                                                            ccGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATCTTATTGCGATa                                            >  2:321313/1‑139 (MQ=255)                                                                              gTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATCTTATTGCGATAAt                                          <  2:1117506/139‑1 (MQ=255)                                                                                            tACGTTTAACGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAg                            >  2:1131004/1‑139 (MQ=255)                                                                                                   aaCGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATCTTa                                                    <  2:1188186/108‑1 (MQ=255)                                                                                                   aaCGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATCTTa                                                    >  1:1188186/1‑108 (MQ=255)                                                                                                    aCGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTGAagcc                    >  1:498965/1‑139 (MQ=255)                                                                                                     cGATCCTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTGAcgccc                   <  2:171305/139‑1 (MQ=255)                                                                                                         ccTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCgcca               <  2:399024/139‑1 (MQ=255)                                                                                                         ccTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCgcca               >  2:507997/1‑139 (MQ=255)                                                                                                          cTTCCAGACCTTCCACCACTGCTTTCGGCACGTTATCCCCCTGATAGAGATCGGTGACGACTGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGTTGACGCCCgctag              >  1:805129/1‑139 (MQ=255)                                                                                                          cTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATCTTATTGCGATAAt                                          >  2:990984/1‑111 (MQ=255)                                                                                                          cTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATCTTATTGCGATAAt                                          <  1:990984/111‑1 (MQ=255)                                                                                                          cTTCCAGACCTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCgccag              >  2:270058/1‑139 (MQ=255)                                                                                                                  ccTTCAACCACTGCTTTCGGCTCGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATGTTATTCCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCCCCCGt      >  1:733573/1‑139 (MQ=255)                                                                                                                  ccTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCAGTGCCGACTGCGTTTTGCCCT‑CAGCCACATAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTc     >  1:1044077/1‑139 (MQ=255)                                                                                                                   cTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGt      <  2:10760/138‑1 (MQ=255)                                                                                                                   cTTCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGt      >  1:10760/1‑138 (MQ=255)                                                                                                                    ttCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTcg    >  2:1013689/1‑139 (MQ=255)                                                                                                                     tCAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTcgc   >  2:871401/1‑139 (MQ=255)                                                                                                                      cAACCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCGGCCACCCGTcgcg  >  2:66643/1‑139 (MQ=255)                                                                                                                       aaCCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGc                                                                  >  2:1026328/1‑74 (MQ=255)                                                                                                                       aaCCACTGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGc                                                                  <  1:1026328/74‑1 (MQ=255)                                                                                                                             |                                                                                                                                    GGATGATCGACAAACGGTCGCCCGTGGTTTTGTTTTCACTCTTCAGCATATAAGTGATGTTATTGGTCCACATCACCGTTTCGCTAACCGGTACGTTTAACGATCCTTCCAGACCTTCAACCACCGCTTTCGGCACGTTATCCCACTGATAGAGATCGGTGCCGACTGCGTTTTGCCCTACAGCCACATAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTCGCG  >  NC_000913/610622‑610877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑