Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	66,447	T→C	E35G (GAG→GGG)	araD ←	L‑ribulose‑5‑phosphate 4‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	66,447	0	T	C	100.0%	177.0 / NA	49	E35G (GAG→GGG)	araD	L‑ribulose‑5‑phosphate 4‑epimerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/28);  total (21/28)

GGCGTGTCGGAGGAGGGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCG  >  NC_000913/66318‑66574                                                                                                                                  |                                                                                                                                ggCGTGTCGGAGGAGGGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATTCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCaa                                                                                                                        <  1:443002/139‑1 (MQ=255)     tgtCGGAGGAGGGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGgc                                                                                                                    >  2:901159/1‑139 (MQ=255)       tCGGAGGAGGGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGgcgc                                                                                                                  <  1:991750/139‑1 (MQ=255)       tCGGAGGAGGGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGgcgc                                                                                                                  >  1:1000308/1‑139 (MQ=255)         ggaggaGGGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTg                                                                                                                >  2:909413/1‑139 (MQ=255)           aggaggGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGAc                                                                                                              <  1:1083803/139‑1 (MQ=255)            ggaggGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACg                                                                                                             <  1:908729/139‑1 (MQ=255)             gaggGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGt                                                                                                            <  1:901159/139‑1 (MQ=255)             gaggGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGt                                                                                                            <  1:75780/139‑1 (MQ=255)               gggCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTg                                                                                                          <  1:879541/139‑1 (MQ=255)               gggCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTg                                                                                                          <  1:508776/139‑1 (MQ=255)                gtcTTTTTCGTACCTTCAACCACTTCACGGGTGTCGATGCTAATCACGACCATATCGTCAGCGGTCATGTCGCTGTAATCGACGCCGGACGGTTTGATCAAAAAAACGCTGCGCCCGCTATCAACGGCGCTGGCGTTGc                                                                                                         >  1:403122/3‑139 (MQ=255)                 gCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGcc                                                                                                        <  2:465760/139‑1 (MQ=255)                  cTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGccc                                                                                                       >  1:1034512/1‑139 (MQ=255)                               tCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGa                                                                                          <  1:904041/139‑1 (MQ=255)                                       ttCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTtg                                                                                  >  1:739652/1‑139 (MQ=255)                                       ttCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTtg                                                                                  >  1:1147824/1‑139 (MQ=255)                                         cACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTtgtg                                                                                >  1:820262/1‑139 (MQ=255)                                         cACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTtgtg                                                                                >  2:887546/1‑139 (MQ=255)                                            cGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGttt                                                                             >  2:345468/1‑139 (MQ=255)                                                         aaCCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAgg                                                                >  2:109192/1‑139 (MQ=255)                                                         aaCCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAgg                                                                >  1:854064/1‑139 (MQ=255)                                                            cACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTg                                                             <  2:854064/139‑1 (MQ=255)                                                             aCGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTgg                                                            <  1:887546/139‑1 (MQ=255)                                                                         tCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCt                                                <  1:909413/139‑1 (MQ=255)                                                                           aGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTgg                                                            <  1:942112/125‑1 (MQ=255)                                                                           aGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTgg                                                            >  2:942112/1‑125 (MQ=255)                                                                           aGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTgg                                              <  2:739652/139‑1 (MQ=255)                                                                                 cATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTg                                        <  2:1034512/139‑1 (MQ=255)                                                                                   tGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGAc                                                                                                              <  2:1049256/67‑1 (MQ=255)                                                                                   tGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGAc                                                                                                              >  1:1049256/1‑67 (MQ=255)                                                                                   tGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTgag                                      <  2:1000308/139‑1 (MQ=255)                                                                                          gTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGAtcttct                               <  2:820262/139‑1 (MQ=255)                                                                                                  cGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATAcc                                                 >  1:639789/1‑113 (MQ=255)                                                                                                  cGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATAcc                                                 <  2:639789/113‑1 (MQ=255)                                                                                                   gCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGt                                                                                    <  1:1067524/77‑1 (MQ=255)                                                                                                   gCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGt                                                                                    >  2:1067524/1‑77 (MQ=255)                                                                                                   gCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGt                                                                                    <  2:571954/77‑1 (MQ=255)                                                                                                   gCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGt                                                                                    >  1:571954/1‑77 (MQ=255)                                                                                                     cGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGt                                                                                                            <  2:249036/51‑1 (MQ=255)                                                                                                     cGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGt                                                                                                            >  1:249036/1‑51 (MQ=255)                                                                                                     cGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGTGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGAc                    >  2:218633/1‑139 (MQ=255)                                                                                                                     aaaGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGTGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATg    <  1:218633/139‑1 (MQ=255)                                                                                                                     aaaGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGAc                    <  2:734004/123‑1 (MQ=255)                                                                                                                     aaaGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGAc                    >  1:734004/1‑123 (MQ=255)                                                                                                                       aGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATgcg  <  1:109192/139‑1 (MQ=255)                                                                                                                          cgccgcGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGAtcttct                               <  2:1172880/107‑1 (MQ=255)                                                                                                                          cgccgcGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGAtcttct                               >  1:1172880/1‑107 (MQ=255)                                                                                                                               cgcCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATgcg  <  1:1092463/131‑1 (MQ=255)                                                                                                                                  |                                                                                                                                GGCGTGTCGGAGGAGGGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCG  >  NC_000913/66318‑66574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑