Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	69,078	A→G	V324A (GTG→GCG)	araB ←	L‑ribulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	69,078	0	A	G	100.0%	116.1 / NA	33	V324A (GTG→GCG)	araB	L‑ribulokinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/16);  total (17/16)

GCGTTGATTTGCGTTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCA  >  NC_000913/68943‑69209                                                                                                                                        |                                                                                                                                    gcGTTGATTTGCGTTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCgcgc                                                                                                                                  <  1:101114/139‑1 (MQ=255) gcGTTGATTTGCGTTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCgcgc                                                                                                                                  <  2:978233/139‑1 (MQ=255) gcGTTGATTTGCGTTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCgcgc                                                                                                                                  <  1:954201/139‑1 (MQ=255)           gCGTTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAAc                                                                                                                        <  2:398009/139‑1 (MQ=255)                      tCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCaaa                                                                                                             >  1:727969/1‑139 (MQ=255)                          gATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCaa                                                                                                              <  1:432181/134‑1 (MQ=255)                          gATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCaa                                                                                                              >  2:432181/1‑134 (MQ=255)                                                  gCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGct                                                                                 <  1:396253/139‑1 (MQ=255)                                                            gAGTACGCGACCAAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTc                                                                        <  2:485578/138‑1 (MQ=255)                                                            gAGTACGCGACCAAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTc                                                                        >  1:485578/1‑138 (MQ=255)                                                             aGTACGCGACCAAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCgg                                                                      <  1:1196662/139‑1 (MQ=255)                                                               tACGCGACCAAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCCTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCa                                                                    >  1:402836/1‑139 (MQ=255)                                                                             aGGCGTAGATATCACCAAACGCCGATTGGCCTGCGTCCAGACCGAGAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCa                                                      <  2:402836/139‑1 (MQ=255)                                                                                        tCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTc                                                         <  2:486359/125‑1 (MQ=255)                                                                                        tCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTc                                                         >  1:486359/1‑125 (MQ=255)                                                                                         cACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTAcc                                          >  1:10046/1‑139 (MQ=255)                                                                                         cACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTAcc                                          >  2:247306/1‑139 (MQ=255)                                                                                           ccAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGa                                        <  2:727969/139‑1 (MQ=255)                                                                                               aCGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCg                                                                                             <  1:678602/82‑1 (MQ=255)                                                                                               aCGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCg                                                                                             >  2:678602/1‑82 (MQ=255)                                                                                                cGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCa                                                      <  2:10841/120‑1 (MQ=255)                                                                                                cGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCa                                                      >  1:10841/1‑120 (MQ=255)                                                                                                  ccGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACttt                                 <  2:302617/139‑1 (MQ=255)                                                                                                   cGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGa                                                                                                                    <  1:766143/55‑1 (MQ=255)                                                                                                   cGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGa                                                                                                                    >  2:766143/1‑55 (MQ=255)                                                                                                      ttGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTAcc                             >  2:937465/1‑139 (MQ=255)                                                                                                       tGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCa                            <  1:937465/139‑1 (MQ=255)                                                                                                          ccTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTg                         >  2:1207065/1‑139 (MQ=255)                                                                                                           cTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGc                        >  2:579904/1‑139 (MQ=255)                                                                                                               ttCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGaa                                                             <  2:347209/98‑1 (MQ=255)                                                                                                               ttCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGaa                                                             >  1:347209/1‑98 (MQ=255)                                                                                                                    gACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCg                                                        <  1:548836/98‑1 (MQ=255)                                                                                                                    gACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCg                                                        >  2:548836/1‑98 (MQ=255)                                                                                                                        gATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCaa                                                                                                              <  2:1135945/40‑1 (MQ=255)                                                                                                                        gATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCaa                                                                                                              >  1:1135945/1‑40 (MQ=255)                                                                                                                                 aGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCa  >  1:218415/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                    GCGTTGATTTGCGTTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCCTGCGCCA  >  NC_000913/68943‑69209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑