Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	681,418	C→T	R403C (CGT→TGT)	djlC →	J domain‑containing HscC co‑chaperone, Hsc56

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	681,418	0	C	T	100.0%	200.6 / NA	62	R403C (CGT→TGT)	djlC	J domain‑containing HscC co‑chaperone, Hsc56
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/28);  total (34/28)

AGCGCAAAGCATGGGCGTGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCCGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCC‑GGAGGATGAAGATGTCGATGAGCCAACACCTGAAAATCATG  >  NC_000913/681287‑681547                                                                                                                                    |                                                                                                                                   aGCGCAAAGCATGGGCGTGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTaa                                                                                                                             >  2:582530/1‑139 (MQ=255)  gcgcAAAGCATGGGCGTGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAAt                                                                                                                            >  2:526353/1‑139 (MQ=255)                  tggtggAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCg                                                                                                            >  1:515139/1‑139 (MQ=255)                    gtggAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGga                                                                                                          >  1:141147/1‑139 (MQ=255)                    gtggAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGga                                                                                                          >  1:551174/1‑139 (MQ=255)                       gAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGAATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGAt                                                                                                       >  1:1028814/1‑139 (MQ=255)                         aaTCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGc                                                                                                     <  2:622905/139‑1 (MQ=255)                         aaTCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGc                                                                                                     >  1:622905/1‑139 (MQ=255)                          aTCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCt                                                                                                    <  2:515139/139‑1 (MQ=255)                              agagTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATc                                                                                                >  1:568721/1‑139 (MQ=255)                                         cGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGcc                                                                                     >  1:149037/1‑139 (MQ=255)                                           agaCAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAATGCGGAGATGCTc                                                                                                   <  2:638009/123‑1 (MQ=255)                                           agaCAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAATGCGGAGATGCTc                                                                                                   >  1:638009/1‑123 (MQ=255)                                            gaCAGAGCAAGATTCACCGTTCCTGGATTTGAATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCAtt                                                                                  <  2:1028814/139‑1 (MQ=255)                                             aCAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTg                                                                                 >  2:652543/1‑139 (MQ=255)                                              cAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGt                                                                                >  1:65887/1‑139 (MQ=255)                                                 agCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCAc                                                                             >  1:753169/1‑139 (MQ=255)                                                  gCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACa                                                                                                                         <  2:881511/94‑1 (MQ=255)                                                  gCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACa                                                                                                                         >  1:881511/1‑94 (MQ=255)                                                      gATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACATGGCTGGAAGGCGGAGATGCTc                                                                                                   >  2:1122798/1‑112 (MQ=255)                                                      gATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTc                                                                                                   <  1:1122798/112‑1 (MQ=255)                                                      gATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGa                                                                        >  2:278355/1‑139 (MQ=255)                                                      gATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGa                                                                        >  2:570837/1‑139 (MQ=255)                                                           aCCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCtt                                                                   >  2:286309/1‑139 (MQ=255)                                                             cGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCtttt                                                                 >  2:201134/1‑139 (MQ=255)                                                                       tttGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCG‑TTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGa                                                      >  2:250817/1‑139 (MQ=255)                                                                           tATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAAc                                                   >  2:1195322/1‑139 (MQ=255)                                                                                ggTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAg                                              <  1:614727/139‑1 (MQ=255)                                                                                 gTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGc                                             <  1:570837/139‑1 (MQ=255)                                                                                  tAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGcc                                            >  1:339149/1‑139 (MQ=255)                                                                                  tAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGcc                                            <  1:392844/139‑1 (MQ=255)                                                                                   aGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCC‑g                                          <  1:1195322/139‑1 (MQ=255)                                                                                   aGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCC‑g                                          <  2:551174/139‑1 (MQ=255)                                                                                   aGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCC‑g                                          <  2:65887/139‑1 (MQ=255)                                                                                   aGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCC‑g                                          <  2:800159/139‑1 (MQ=255)                                                                                   aGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCC‑g                                          <  1:652543/139‑1 (MQ=255)                                                                                         aCAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCC‑GGAGGAt                                    <  1:582530/139‑1 (MQ=255)                                                                                               agtaCTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATa                                                                                                                           >  2:596020/1‑47 (MQ=255)                                                                                               agtaCTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATa                                                                                                                           <  1:596020/47‑1 (MQ=255)                                                                                               agtaCTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGa                                                                        <  2:714166/98‑1 (MQ=255)                                                                                               agtaCTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGa                                                                        >  1:714166/1‑98 (MQ=255)                                                                                               agtaCTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCC‑GGAGGATGAAGAt                              <  1:201134/139‑1 (MQ=255)                                                                                                  aCTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCAtt                                                                                            <  1:390048/75‑1 (MQ=255)                                                                                                  aCTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCAtt                                                                                            >  2:390048/1‑75 (MQ=255)                                                                                                    ttGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCC‑GGAGGATGAAGATGTCGa                         >  2:513458/1‑139 (MQ=255)                                                                                                    ttGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCC‑GGAGGATGAAGATGTCGa                         <  1:222129/139‑1 (MQ=255)                                                                                                      gAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATg                                                                      <  1:1004704/93‑1 (MQ=255)                                                                                                      gAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATg                                                                      >  2:1004704/1‑93 (MQ=255)                                                                                                      gAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCCGGGAGGATGAAGATGTCGAt                        >  1:646039/1‑139 (MQ=255)                                                                                                      gAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCC‑GGAGGATGAAGATGTCGATg                       >  1:375772/1‑139 (MQ=255)                                                                                                       aaGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGa                                                               >  2:970095/1‑99 (MQ=255)                                                                                                       aaGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGa                                                               <  1:970095/99‑1 (MQ=255)                                                                                                       aaGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCC‑g                                          >  2:487961/1‑119 (MQ=255)                                                                                                       aaGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCC‑g                                          <  1:487961/119‑1 (MQ=255)                                                                                                        aGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCC‑GGAGGATGAAGATGTCGATGAg                     >  1:401928/1‑139 (MQ=255)                                                                                                         gCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCC‑GGAGGATGAAGATGTCGATGAGc                    <  1:278355/139‑1 (MQ=255)                                                                                                          cGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCC‑GGAGGATGAAGATGTCGATGAGcc                   <  1:513458/139‑1 (MQ=255)                                                                                                           gTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGATGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCC‑GGAGGATGAAGATGTCGATGAGCCa                  >  1:125299/1‑139 (MQ=255)                                                                                                            tttCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCC‑GGAGGATGAAGATGTCGATGAGCCaa                 >  2:410081/1‑139 (MQ=255)                                                                                                            tttCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCC‑GGAGGATGAAGATGTCGATGAGCCaa                 <  2:339149/139‑1 (MQ=255)                                                                                                               ccGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCC‑GGAGGATGAAGATGTCGATGAGCCAacac              <  1:1056540/139‑1 (MQ=255)                                                                                                                           aTGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCC‑GGAGGATGAAGATGTCGATGAGCCAACACCTGAAAATCATg  <  2:149037/139‑1 (MQ=255)                                                                                                                                    |                                                                                                                                   AGCGCAAAGCATGGGCGTGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCCGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCC‑GGAGGATGAAGATGTCGATGAGCCAACACCTGAAAATCATG  >  NC_000913/681287‑681547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑