Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	716,388	C→T	pseudogene (210/651 nt)	ybfG ←	pseudogene

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	716,388	0	C	T	100.0%	163.0 / NA	51	pseudogene (210/651 nt)	ybfG	pseudogene
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/21);  total (30/21)

TTAAGAGCAAAAATTACCCCTGCGACATCGTCAGCGATGGGTAAAGCCCGCACGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAACTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATCTTTATTAACCGGTAAGAGTTCGGTATGGTTT  >  NC_000913/716257‑716520                                                                                                                                    |                                                                                                                                     ttAAGAGCAAAAATTACCCCTGCGACATCGTCAGCGATGGGTAAAGCCCGCACGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCAt                                                                                                                               <  2:943170/139‑1 (MQ=255)         aaaaaTTACCCCTGCGACATCGTCAGCGATGGGTAAAGCCCGCACGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTatcaatc                                                                                                                       >  1:1035500/1‑139 (MQ=255)         aaaaaTTACCCCTGCGACATCGTCAGCGATGGGTAAAGCCCGCACGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTatcaatc                                                                                                                       >  1:805270/1‑139 (MQ=255)         aaaaaTTACCCCTGCGACATCGTCAGCGATGGGTAAAGCCCGCACGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTatcaatc                                                                                                                       >  2:208385/1‑139 (MQ=255)                     tGCGACATCGTCAGCGATGGGTAAAGCCCGCACGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCg                                                                                                           <  1:1163694/139‑1 (MQ=255)                     tGCGACATCGTCAGCGATGGGTAAAGCCCGCACGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCg                                                                                                           <  2:1035500/139‑1 (MQ=255)                      gCGACATCGTCAGCGATGGGTAAAGCCCGCACGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCgg                                                                                                          <  1:768522/139‑1 (MQ=255)                       cGACATCGTCAGCGATGGGTAAAGCCCGCACGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGa                                                                                                         >  1:24914/1‑139 (MQ=255)                             cGTCAGCGATGGGTAAAGCCCGCACGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATa                                                                                                   >  2:601240/1‑139 (MQ=255)                                    gATGGGTAAAGCCCGCACGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAgc                                                                                            <  1:605423/139‑1 (MQ=255)                                            aaGCCCGCACGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCAt                                                                                    <  1:208385/139‑1 (MQ=255)                                                   cACGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGAt                                                                             >  1:990853/1‑139 (MQ=255)                                                   cACGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGAt                                                                             >  1:884129/1‑139 (MQ=255)                                                   cACGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGAt                                                                             >  1:836399/1‑139 (MQ=255)                                                   cACGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGAt                                                                             >  1:1080347/1‑139 (MQ=255)                                                       ccTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCCCCGGAGCCATAGACAAGCGCACCCATCGGTGATTttg                                                                         <  1:942834/139‑1 (MQ=255)                                                              aTTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCAc                                                                  <  2:1080347/139‑1 (MQ=255)                                                                        ttGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGt                                                                                <  1:959001/115‑1 (MQ=255)                                                                        ttGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGt                                                                                >  2:959001/1‑115 (MQ=255)                                                                          gtgtCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATa                                                      >  1:454570/1‑139 (MQ=255)                                                                                     aCTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGttt                                           >  2:449142/1‑139 (MQ=255)                                                                                     aCTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGttt                                           >  2:344278/1‑139 (MQ=255)                                                                                     aCTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGttt                                           >  2:338100/1‑139 (MQ=255)                                                                                          aTCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCt                                      <  2:884129/139‑1 (MQ=255)                                                                                          aTCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCt                                      <  1:338100/139‑1 (MQ=255)                                                                                            ctcGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAg                                                                                                        <  1:256995/71‑1 (MQ=255)                                                                                            ctcGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAg                                                                                                        >  2:256995/1‑71 (MQ=255)                                                                                              cGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAAt                                  >  2:1004627/1‑139 (MQ=255)                                                                                                 ggCAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATCtt                               >  1:22833/1‑139 (MQ=255)                                                                                                   cAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAgcgc                                                                                          >  2:928164/1‑78 (MQ=255)                                                                                                   cAACCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAgcgc                                                                                          <  1:928164/78‑1 (MQ=255)                                                                                                     aCCGGGGGTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATCTttatt                           >  2:1141810/1‑139 (MQ=255)                                                                                                            gTGACCAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATCTTTATTAACCGGt                    <  1:1141810/139‑1 (MQ=255)                                                                                                                ccAGATCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATCTTTATTAACCGGTAaga                <  2:805270/139‑1 (MQ=255)                                                                                                                    aTCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATCTTTATTAACCGGTAagag               <  1:736674/136‑1 (MQ=255)                                                                                                                    aTCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATCTTTATTAACCGGTAagag               >  2:736674/1‑136 (MQ=255)                                                                                                                    aTCCTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATCTTTATTAACCGGTAAGAGTTc            >  2:958570/1‑139 (MQ=255)                                                                                                                       cTGCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATCTTTATTAACCGGTAAGAGTTCGGt         <  1:449142/139‑1 (MQ=255)                                                                                                                         gCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCt                                      <  2:693358/108‑1 (MQ=255)                                                                                                                         gCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCt                                      >  1:693358/1‑108 (MQ=255)                                                                                                                         gCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATCTTTATTAACCGGTAAGAGTTCGGTAt       >  1:104833/1‑139 (MQ=255)                                                                                                                         gCTATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATCTTTATTAACCGGTAAGAGTTCGGTAt       >  1:1182406/1‑139 (MQ=255)                                                                                                                           tATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCt                                                                >  2:328346/1‑80 (MQ=255)                                                                                                                           tATGCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCt                                                                <  1:328346/80‑1 (MQ=255)                                                                                                                              gCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGAtt                                                                            >  2:436328/1‑65 (MQ=255)                                                                                                                              gCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGAtt                                                                            <  1:436328/65‑1 (MQ=255)                                                                                                                              gCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATCTTTATTaa                         >  2:648572/1‑116 (MQ=255)                                                                                                                              gCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATCTTTATTaa                         <  1:648572/116‑1 (MQ=255)                                                                                                                              gCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATCTTTATTAACCGGTAAGAGTTCGGTATGGttt  >  2:657696/1‑139 (MQ=255)                                                                                                                              gCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATCTTTATTAACCGGTAAGAGTTCGGTATGGttt  >  1:183000/1‑139 (MQ=255)                                                                                                                              gCGTAATTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATCTTTATTAACCGGTAAGAGTTCGGTATGGttt  >  2:11676/1‑139 (MQ=255)                                                                                                                                    |                                                                                                                                     TTAAGAGCAAAAATTACCCCTGCGACATCGTCAGCGATGGGTAAAGCCCGCACGCCTGCGAATTCAGGGCGTTGTGTCCAACTTACTGGATCTCGTGGCAACCGGGGGTGACCAGATCCTGCTATGCGTAACTAACCATTATCAATCAGCAGACCTCCGGAGCCATAGACAAGCGCACCCATCGGTGATTTTGTTGTCACCTGTAATTGATAAAGTGCGGTTTCGGCTAAATCTTTATTAACCGGTAAGAGTTCGGTATGGTTT  >  NC_000913/716257‑716520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑