Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	753,428	C→T	A347A (GCG→GCA)	gltA ←	citrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	753,428	0	C	T	100.0%	120.7 / NA	39	A347A (GCG→GCA)	gltA	citrate synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/21);  total (18/21)

AACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGCGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCACTTCATGGCAGGTTTCACGCATTACGGTGGCGCGCGGGTCGTAATTTTTGTACACGCGGTGACCG  >  NC_000913/753299‑753557                                                                                                                                  |                                                                                                                                  aaCGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGttt                                                                                                                          <  1:661850/139‑1 (MQ=255)    ggTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTcc                                                                                                                       >  1:1076111/1‑139 (MQ=255)                tGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGcc                                                                                                           >  2:729239/1‑139 (MQ=255)                 gCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCa                                                                                                          <  2:125214/139‑1 (MQ=255)                       aTGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCca                                                                                                    <  2:385073/139‑1 (MQ=255)                                     ggAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTc                                                                                      <  1:917883/139‑1 (MQ=255)                                        aGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTc                                                                                      >  2:1177376/1‑136 (MQ=255)                                        aGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTc                                                                                      <  1:1177376/136‑1 (MQ=255)                                            ggAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCca                                                                                                    <  2:670556/118‑1 (MQ=255)                                            ggAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCca                                                                                                    >  1:670556/1‑118 (MQ=255)                                            ggAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCa                                                                               >  1:1141821/1‑139 (MQ=255)                                                 aCCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGctct                                                                          <  2:1141821/139‑1 (MQ=255)                                                   ccATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCttt                                                                        <  2:1076111/139‑1 (MQ=255)                                                      tCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAg                                                                     >  1:307345/1‑139 (MQ=255)                                                        gCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCa                                                                   <  2:1098863/139‑1 (MQ=255)                                                            tCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCcag                                                                                                   <  1:372708/103‑1 (MQ=255)                                                            tCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCcag                                                                                                   >  2:372708/1‑103 (MQ=255)                                                                      aCCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGACAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCACTTCATGGCAGGtt                                                     <  1:729239/139‑1 (MQ=255)                                                                          gagTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGctct                                                                          <  2:24812/114‑1 (MQ=255)                                                                          gagTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGctct                                                                          >  1:24812/1‑114 (MQ=255)                                                                             tAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCACTTCATGGCAGGTTTCACGCa                                              >  2:513788/1‑139 (MQ=255)                                                                             tAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCACTTCATGGCAGGTTTCACGCa                                              >  2:538188/1‑139 (MQ=255)                                                                               gAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCACTTCATGGCAGGTTTCACGCAtt                                            >  2:109703/1‑139 (MQ=255)                                                                                     gtcGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCcagc                                                                                                  <  2:752145/77‑1 (MQ=255)                                                                                     gACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCT‑TTTCAGCACTTCATGGCAGGTTTCACg                                                >  2:103913/1‑128 (MQ=255)                                                                                     gACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCT‑TTTCAGCACTTCATGGCAGGTTTCACg                                                <  1:103913/128‑1 (MQ=255)                                                                                     gACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTACAGTGCGATGTTTTCCAGCTCCATAGCCACTTCcagc                                                                                                  >  1:752145/1‑79 (MQ=255)                                                                                          tCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGTACTTCATGGCAGGTTTCACGCATTACGGTGgcgcg                                 <  1:755229/139‑1 (MQ=255)                                                                                          tCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCACTTCATGGCAGGTTTCa                                                  <  2:2881/122‑1 (MQ=255)                                                                                          tCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCACTTCATGGCAGGTTTCa                                                  >  1:2881/1‑122 (MQ=255)                                                                                                              aTAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCACTTCATGGCAGGTTTCACGCATTACGGTGGCGCGCGGGTCGTAATTTTTGTa               >  1:951921/1‑137 (MQ=255)                                                                                                              aTAAAGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCACTTCATGGCAGGTTTCACGCATTACGGTGGCGCGCGGGTCGTAATTTTTGTa               <  2:951921/137‑1 (MQ=255)                                                                                                                aaaGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCACTTCATGGCAGGTTTCACGCATTACGGTGgcgcgc                                >  2:407790/1‑118 (MQ=255)                                                                                                                aaaGTACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCACTTCATGGCAGGTTTCACGCATTACGGTGgcgcgc                                <  1:407790/118‑1 (MQ=255)                                                                                                                    tACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCAt                                                                                           <  2:1090255/55‑1 (MQ=255)                                                                                                                    tACGGGTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCAt                                                                                           >  1:1090255/1‑55 (MQ=255)                                                                                                                         gTCGTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCACTTCATGGCAGGTTTCACGCATTACGGTGGCGCGCGGGTCGTAATTTTTGTACACGCGGTGACCg  <  1:513788/139‑1 (MQ=255)                                                                                                                            gTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCACTTCATGGCAGGTTTc                                                   <  2:33246/87‑1 (MQ=255)                                                                                                                            gTTCAGTGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCACTTCATGGCAGGTTTc                                                   >  1:33246/1‑87 (MQ=255)                                                                                                                                  |                                                                                                                                  AACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGCGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCACTTCATGGCAGGTTTCACGCATTACGGTGGCGCGCGGGTCGTAATTTTTGTACACGCGGTGACCG  >  NC_000913/753299‑753557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑