Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	762,739	G→A	*406* (TAG→TAA)	sucB →	dihydrolipoyltranssuccinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	762,739	0	G	A	100.0%	162.5 / NA	50	*406* (TAG→TAA)	sucB	dihydrolipoyltranssuccinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/28);  total (22/28)

ATCCTGCCGATGATGTACCTGGCGCTGTCCTACGATCACCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAGTAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAAT  >  NC_000913/762611‑762850                                                                                                                                 |                                                                                                                aTCCTGCCGATGATGTACCTGGCGCTGTCCTACGATCACCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGt                                                                                                       <  1:755221/139‑1 (MQ=255)     tGCCGATGATGTACCTGGCGCTGTCCTACGATCACCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGt                                                                                                       >  2:159242/1‑135 (MQ=255)     tGCCGATGATGTACCTGGCGCTGTCCTACGATCACCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGt                                                                                                       <  1:159242/135‑1 (MQ=255)     tGCCGATGATGTACCTGGCGCTGTCCTACGATCACCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCa                                                                                                   >  2:161299/1‑139 (MQ=255)      gCCGATGATGTACCTGGCGCTGTCCTACGATCACCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCAc                                                                                                  >  2:123750/1‑139 (MQ=255)      gCCGATGATGTACCTGGCGCTGTCCTACGATCACCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCAc                                                                                                  <  2:1067019/139‑1 (MQ=255)        cGATGATGTACCTGGCGCTGTCCTACGATCACCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCt                                                                                                <  1:123750/139‑1 (MQ=255)         gatgatGTACCTGGCGCTGTCCTACGATCACCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTg                                                                                               <  2:457903/139‑1 (MQ=255)             atgTACCTGGCGCTGTCCTACGATCACCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACt                                                                                           <  2:627546/139‑1 (MQ=255)                          tGTCCTACGATCACCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATaa                                                                              >  2:87995/1‑139 (MQ=255)                                      aCCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGcc                                                                  >  1:1075469/1‑139 (MQ=255)                                              aTCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTa                                                                                                          <  2:668846/91‑1 (MQ=255)                                              aTCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTa                                                                                                          >  1:668846/1‑91 (MQ=255)                                              aTCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGt                                                                                                       <  2:737920/94‑1 (MQ=255)                                              aTCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGt                                                                                                       >  1:737920/1‑94 (MQ=255)                                              aTCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGAc                                                                                     <  1:459468/112‑1 (MQ=255)                                              aTCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGAc                                                                                     >  1:507397/1‑112 (MQ=255)                                              aTCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGAc                                                                                     >  2:459468/1‑112 (MQ=255)                                              aTCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGAc                                                                                     <  2:507397/112‑1 (MQ=255)                                                        gcgAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCa                                                                          >  2:832948/1‑113 (MQ=255)                                                        gcgAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCa                                                                          <  1:832948/113‑1 (MQ=255)                                                         cgAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGcc                                               <  1:161299/139‑1 (MQ=255)                                                              ccGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATg                                          <  1:531553/139‑1 (MQ=255)                                                               cGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCgg                                                          <  1:28954/122‑1 (MQ=255)                                                               cGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCgg                                                          >  2:28954/1‑122 (MQ=255)                                                               cGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGc                                         >  2:242667/1‑139 (MQ=255)                                                                     cTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCt                                   <  1:242667/139‑1 (MQ=255)                                                                     cTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCt                                   <  1:58231/139‑1 (MQ=255)                                                                        ccTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAg                                                                             <  2:1209507/94‑1 (MQ=255)                                                                        ccTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAg                                                                             >  1:1209507/1‑94 (MQ=255)                                                                          tGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGAcgc                              <  2:250366/139‑1 (MQ=255)                                                                                  aTCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATc                      >  2:233843/1‑139 (MQ=255)                                                                                    cAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAg                    <  1:799839/139‑1 (MQ=255)                                                                                    cAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAg                    >  2:418052/1‑139 (MQ=255)                                                                                            tGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACgg            <  1:233843/139‑1 (MQ=255)                                                                                             gCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCAc                                                                                                  >  2:1135821/1‑52 (MQ=255)                                                                                             gCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCAc                                                                                                  <  1:1135821/52‑1 (MQ=255)                                                                                             gCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACggg           <  2:335028/139‑1 (MQ=255)                                                                                             gCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACggg           >  2:440354/1‑139 (MQ=255)                                                                                             gCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACggg           >  1:844808/1‑139 (MQ=255)                                                                                               tGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGac         <  2:844808/139‑1 (MQ=255)                                                                                                    gATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTGCGGGAccacca    >  2:203910/1‑139 (MQ=255)                                                                                                     aTCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGt                            <  2:1122266/114‑1 (MQ=255)                                                                                                     aTCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGt                            >  1:1122266/1‑114 (MQ=255)                                                                                                      tCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAAt  <  2:13390/139‑1 (MQ=255)                                                                                                      tCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAAt  <  1:361008/139‑1 (MQ=255)                                                                                                        cGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAg                    <  2:1217218/119‑1 (MQ=255)                                                                                                        cGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAg                    >  1:1217218/1‑119 (MQ=255)                                                                                                                 tgctgctgGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTa               <  1:78293/115‑1 (MQ=255)                                                                                                                 tgctgctgGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTa               >  2:78293/1‑115 (MQ=255)                                                                                                                              gtaataGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTcc                                                            <  2:868997/57‑1 (MQ=255)                                                                                                                                 |                                                                                                                ATCCTGCCGATGATGTACCTGGCGCTGTCCTACGATCACCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAGTAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAAT  >  NC_000913/762611‑762850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑