Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	72,229	C→T	*233* (TAG→TAA)	thiQ ←	thiamine/thiamine pyrophosphate ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	72,229	0	C	T	100.0%	182.0 / NA	56	*233* (TAG→TAA)	thiQ	thiamine/thiamine pyrophosphate ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/29);  total (27/29)

AGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTTCCACG  >  NC_000913/72100‑72361                                                                                                                                  |                                                                                                                                     aGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTa                                                                                                                             <  1:247046/139‑1 (MQ=255)      ttGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATccc                                                                                                                        >  1:1080942/1‑139 (MQ=255)           gTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATa                                                                                                                   >  2:1135402/1‑139 (MQ=255)               aagagaTAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATc                                                                                                                          <  1:508463/126‑1 (MQ=255)               aagagaTAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATc                                                                                                                          >  2:508463/3‑128 (MQ=255)                     tAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGGTAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGc                                                                                                         <  2:723783/139‑1 (MQ=255)                      aGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCg                                                                                                                               >  1:462000/1‑116 (MQ=255)                      aGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCg                                                                                                                               <  2:462000/116‑1 (MQ=255)                             tgCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCg                                                                                                                               >  1:564203/1‑109 (MQ=255)                             tgCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCg                                                                                                                               <  2:564203/109‑1 (MQ=255)                             tgCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCt                                                                                                 >  1:94530/1‑139 (MQ=255)                                gggTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTAc                                                                                              <  1:1135402/139‑1 (MQ=255)                                  gTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCg                                                                                            >  1:425116/1‑139 (MQ=255)                                  gTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCg                                                                                            <  1:266515/139‑1 (MQ=255)                                  gTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTCCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCg                                                                                            >  2:727360/1‑139 (MQ=255)                                    tGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCt                                                                                          >  1:770101/1‑139 (MQ=255)                                       cTGATGCGACGCTTGCCGCGTCTTATCAGGTCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTcaa                                                                                       >  1:315517/1‑139 (MQ=255)                                              gACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCa                                                                                <  2:1080942/139‑1 (MQ=255)                                              gACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCa                                                                                <  1:727360/139‑1 (MQ=255)                                              gACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCa                                                                                <  1:764852/139‑1 (MQ=255)                                               aCGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCAt                                                                               >  1:435173/1‑139 (MQ=255)                                                cGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCAtt                                                                              >  2:984035/1‑139 (MQ=255)                                                   ttGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGt                                                                           <  2:705794/139‑1 (MQ=255)                                                       cgcgTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATa                                                                       <  1:984035/139‑1 (MQ=255)                                                                   ggCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCg                                                           >  1:473074/1‑139 (MQ=255)                                                                   ggCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCg                                                           >  1:261957/1‑139 (MQ=255)                                                                    gCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGa                                                          >  1:215942/1‑139 (MQ=255)                                                                              cGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTc                                                >  1:801353/1‑139 (MQ=255)                                                                              cGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTc                                                >  2:208550/1‑139 (MQ=255)                                                                                cACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATGCCCAATAGTGCCg                                                                                                             >  1:795440/1‑76 (MQ=255)                                                                                cACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCg                                                                                                             <  2:795440/76‑1 (MQ=255)                                                                                 aCTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGc                                             <  1:208550/139‑1 (MQ=255)                                                                                   tACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGa                                           <  2:801353/139‑1 (MQ=255)                                                                                       cGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGactac                                       <  2:435173/139‑1 (MQ=255)                                                                                           ggTCGGATAAGGTGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTAcc                                   <  1:412954/139‑1 (MQ=255)                                                                                             tCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGa                                 >  2:673331/1‑139 (MQ=255)                                                                                               ggATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCa                                                                                <  2:206807/90‑1 (MQ=255)                                                                                               ggATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCa                                                                                >  1:206807/1‑90 (MQ=255)                                                                                               ggATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAgc                               >  2:224885/1‑139 (MQ=255)                                                                                               ggATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAgc                               >  1:18726/1‑139 (MQ=255)                                                                                                gATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCg                                               >  2:992928/1‑122 (MQ=255)                                                                                                gATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCg                                               <  1:992928/122‑1 (MQ=255)                                                                                                  tAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATgcgc                                                     <  1:452532/114‑1 (MQ=255)                                                                                                  tAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATgcgc                                                     >  2:452532/1‑114 (MQ=255)                                                                                                           cACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAgg                                                             >  1:795100/1‑97 (MQ=255)                                                                                                           cACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAgg                                                             <  2:795100/97‑1 (MQ=255)                                                                                                             cgccgcATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCcgc                 <  2:770101/139‑1 (MQ=255)                                                                                                                cgcATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGcgcc              <  2:215942/139‑1 (MQ=255)                                                                                                                  cATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGcgccgc            <  2:425116/139‑1 (MQ=255)                                                                                                                            tGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGa                                           <  1:322373/98‑1 (MQ=255)                                                                                                                            tGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGa                                           >  2:322373/1‑98 (MQ=255)                                                                                                                            tGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTTCCACg  <  1:224885/139‑1 (MQ=255)                                                                                                                            tGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTTCCACg  >  1:258816/1‑139 (MQ=255)                                                                                                                            tGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTTCCACg  <  2:473074/139‑1 (MQ=255)                                                                                                                            tGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTTCCACg  <  2:18726/139‑1 (MQ=255)                                                                                                                            tGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTTCCACg  <  2:261957/139‑1 (MQ=255)                                                                                                                                  |                                                                                                                                     AGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTTCCACG  >  NC_000913/72100‑72361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑