Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	879,857	G→A	*372* (TAG→TAA)  S208S (AGC→AGT)	yliI → gstB ←	soluble aldose sugar dehydrogenase glutathione S‑transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	879,857	0	G	A	100.0%	101.0 / NA	32	*372* (TAG→TAA)  S208S (AGC→AGT)	yliI gstB	soluble aldose sugar dehydrogenase glutathione S‑transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/15);  total (17/15)

TGGCCGTATTTTAACGGACAGAGGGCAGCGAATTCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAGCTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGCCAACGTTAAACAAATTGTAGATGAACGGTGCGATAG  >  NC_000913/879734‑879987                                                                                                                            |                                                                                                                                   tGGCCGTATTTTAACGGCCAGAGGGCAGCGAATTCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAatcatc                                                                                                                     >  1:696697/1‑139 (MQ=255)                  aCAGAGGGCAGCGAATTCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGc                                                                                                    <  2:730531/139‑1 (MQ=255)                      agGGCAGCGAATTCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCgggg                                                                                                >  1:1040288/1‑139 (MQ=255)                              gAATTCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGt                                                                                        <  1:937255/139‑1 (MQ=255)                                aTTCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTgc                                                                                                           <  1:313815/118‑1 (MQ=255)                                aTTCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTgc                                                                                                           >  2:313815/1‑118 (MQ=255)                                           tCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATAc                                                                           <  2:696697/139‑1 (MQ=255)                                                   gACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTg                                                                   >  2:607748/1‑139 (MQ=255)                                                    aCCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGc                                                                  >  2:151045/1‑139 (MQ=255)                                                         aCGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCgggg                                                                                                <  2:1218530/104‑1 (MQ=255)                                                         aCGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCgggg                                                                                                >  1:1218530/1‑104 (MQ=255)                                                                 ttatACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGAcgcg                                                     <  1:1054302/139‑1 (MQ=255)                                                                 ttatACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGAcgcg                                                     <  2:402598/139‑1 (MQ=255)                                                                          ctcACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGAcgcg                                                     >  1:1056314/1‑130 (MQ=255)                                                                          ctcACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGAcgcg                                                     <  2:1056314/130‑1 (MQ=255)                                                                                  cgaGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGCCa                                    >  1:535856/1‑139 (MQ=255)                                                                                             gggAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGa                                                               <  1:1111138/101‑1 (MQ=255)                                                                                             gggAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGa                                                               >  2:1111138/1‑101 (MQ=255)                                                                                              ggAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGCCAACGTTAAACaaa                        <  2:1032759/139‑1 (MQ=255)                                                                                               gAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGCCAACGTTAAACAAAt                       <  1:607748/139‑1 (MQ=255)                                                                                                  ttACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCa                                                                                          <  2:19631/69‑1 (MQ=255)                                                                                                  ttACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCa                                                                                          >  1:19631/1‑69 (MQ=255)                                                                                                     cTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGCCAACGTTAAACAAATTGTAGa                 >  2:251794/1‑139 (MQ=255)                                                                                                          aGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGAagcg                                                     <  2:829226/98‑1 (MQ=255)                                                                                                          aGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGACTTGCTGATACCAGCGTTGCAGATTTGGAcgcg                                                     >  1:829226/1‑98 (MQ=255)                                                                                                           gTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGCCAACGTTAAACAAATTGTAGATGAACg           <  1:1128984/139‑1 (MQ=255)                                                                                                            ttAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGCCAACGTTAAACAAATTGTAGATGAACgg          >  1:179586/1‑139 (MQ=255)                                                                                                               gCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAg                                                                        <  2:235484/74‑1 (MQ=255)                                                                                                               gCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAg                                                                        >  1:235484/1‑74 (MQ=255)                                                                                                               gCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGCCAACGTTAAACAAATTGTAGATGAACGGTgc       >  2:981757/1‑139 (MQ=255)                                                                                                                    cGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGCCAACGTTAAACAAATTGTAGATGAACGGTgcgatag  >  1:774055/1‑139 (MQ=255)                                                                                                                    cGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGCCAACGTTAAACAAATTGTAGATGAACGGTgcgatag  >  2:663426/1‑139 (MQ=255)                                                                                                                            |                                                                                                                                   TGGCCGTATTTTAACGGACAGAGGGCAGCGAATTCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAGCTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGCCAACGTTAAACAAATTGTAGATGAACGGTGCGATAG  >  NC_000913/879734‑879987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑