Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	909,654	C→T	V466M (GTG→ATG)	poxB ←	pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	909,654	0	C	T	100.0%	176.0 / NA	55	V466M (GTG→ATG)	poxB	pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/32);  total (23/32)

TCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCGCCCATCAACATGCTAAAACCGCCATCGCCGCACATGGCGACCACCTG  >  NC_000913/909524‑909774                                                                                                                                   |                                                                                                                         tcatcaACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCATAAAGCCCa                                                                                                                  <  1:581867/139‑1 (MQ=255)  catcaACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCATAAAGCCCAg                                                                                                                 >  2:884548/1‑139 (MQ=255)  catcaACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCATAAAGCCCAg                                                                                                                 >  2:1100150/1‑139 (MQ=255)    tcaACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCATAAAGCCCAGCa                                                                                                               <  1:361810/139‑1 (MQ=255)    tcaACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCATAAAGCCCAGCa                                                                                                               <  1:689079/139‑1 (MQ=255)     caACTTCAGTCGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCATAAAGCCCAGCAc                                                                                                              <  2:241352/139‑1 (MQ=255)     caACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCATAAAGCCCAGCAc                                                                                                              <  1:884548/139‑1 (MQ=255)      aaCTTCGGACGCGTTTTCTACACGGGTACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTATGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATGGCCACCAGCTTTCATCTCCATCGCCATAAAGCCCAGCACg                                                                                                             <  1:881501/139‑1 (MQ=255)      aaCTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCATAAAGCCCAGCACg                                                                                                             <  1:1100150/139‑1 (MQ=255)        cTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCATAAAGCCCAGCACGCt                                                                                                           <  2:931819/139‑1 (MQ=255)         ttCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCATAAAGCCCAGCACGCtg                                                                                                          >  2:971747/1‑139 (MQ=255)         ttCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCATAAAGCCCAGCACGCtg                                                                                                          <  2:1028053/139‑1 (MQ=255)                                tACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCATAAAGCCCAGCACGCtg                                                                                                          >  1:149010/1‑116 (MQ=255)                                tACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCATAAAGCCCAGCACGCtg                                                                                                          <  2:149010/116‑1 (MQ=255)                                    cGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCATAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGc                                                                               >  2:98907/1‑139 (MQ=255)                                    cGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCATAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGc                                                                               >  2:952302/1‑139 (MQ=255)                                    cGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCATAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGc                                                                               >  1:467289/1‑139 (MQ=255)                                        aTGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCATAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGtt                                                                           >  1:422561/1‑139 (MQ=255)                                         tGCCGCACGCTTCGGCAATGTGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCATAAAGCCCAGCACg                                                                                                             <  1:390414/104‑1 (MQ=255)                                         tGCCGCACGCTTCGGCAATGTGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCATAAAGCCCAGCACg                                                                                                             >  2:390414/1‑104 (MQ=255)                                             gCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCATAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATc                                                                      >  2:654406/1‑139 (MQ=255)                                                cGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCATAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGc                                                                   >  2:329105/1‑139 (MQ=255)                                                 gCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCATAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCTCTGGCAGTTTCATCTGCa                                                                  <  2:801371/139‑1 (MQ=255)                                                 gCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCATAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCa                                                                  <  2:738962/139‑1 (MQ=255)                                                     cGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCATAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCactac                                                              >  1:983054/1‑139 (MQ=255)                                                        cAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCATAAAGCCCAGCa                                                                                                               >  1:248749/1‑87 (MQ=255)                                                        cAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCATAAAGCCCAGCa                                                                                                               <  2:248749/87‑1 (MQ=255)                                                             cGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCATAAAGCCCAGCACGc                                                                                                            >  1:682118/1‑85 (MQ=255)                                                             cGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCATAAAGCCCAGCACGc                                                                                                            <  2:682118/85‑1 (MQ=255)                                                              gggCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCATAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGaa                                                     <  2:467289/139‑1 (MQ=255)                                                               ggCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCATAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGaaa                                                    >  1:154316/1‑139 (MQ=255)                                                               ggCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCATAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGaaa                                                    >  2:64506/1‑139 (MQ=255)                                                                gCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCATAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAAt                                                   <  1:952302/139‑1 (MQ=255)                                                                  aaaGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCATAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCg                                                 <  1:98907/139‑1 (MQ=255)                                                                     gTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCATAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCGccc                                              <  1:971747/139‑1 (MQ=255)                                                                          tgtCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCATAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCGCCCATCaa                                         <  2:154316/139‑1 (MQ=255)                                                                          tgtCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCATAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCGCCCATCaa                                         <  1:64506/139‑1 (MQ=255)                                                                                        gTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCATAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCGCCCATCAACATGCTAAAACCGc                           <  2:637182/139‑1 (MQ=255)                                                                                         tGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCATAAAGCCCa                                                                                                                  <  2:45592/51‑1 (MQ=255)                                                                                         tGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCATAAAGCCCa                                                                                                                  >  1:45592/1‑51 (MQ=255)                                                                                         tGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCATAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGc                                                                   <  1:665352/98‑1 (MQ=255)                                                                                         tGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCATAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGc                                                                   >  2:665352/1‑98 (MQ=255)                                                                                         tGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCATAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCGcc                                               >  2:628688/1‑118 (MQ=255)                                                                                         tGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCATAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCGcc                                               <  1:628688/118‑1 (MQ=255)                                                                                             gtcagtcaAATAGCCACCAGCTTTCATCTCCATCGCCATAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCGCCCATCAACATGCTAAAACCGCCATcg                      <  2:60347/139‑1 (MQ=255)                                                                                                            accaGCTTTCATCTCCATCGCCATAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCGCCCATCAACATGCTAAAACCGCCATcgccg                   >  1:1165946/1‑127 (MQ=255)                                                                                                            accaGCTTTCATCTCCATCGCCATAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCGCCCATCAACATGCTAAAACCGCCATcgccg                   <  2:1165946/127‑1 (MQ=255)                                                                                                                gCTTTCATCTCCATCGCCATAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCGCCCATCAACATGCTAAAACCGCCATCGCCGCACATGGCGACCACCt   <  1:654406/139‑1 (MQ=255)                                                                                                                 cTTTCATCTCCATCGCCATAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCGCCCATCAACATGCTAAAACCGCCATCGCCGCACATGGCGACCACCTg  <  2:422561/139‑1 (MQ=255)                                                                                                                    tCATCTCCATCGCCATAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCGCCCATCAACATGCTAAAACCGCCATCGCCGca                 >  1:64125/1‑121 (MQ=255)                                                                                                                    tCATCTCCATCGCCATAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCGCCCATCAACATGCTAAAACCGCCATCGCCGca                 <  2:64125/121‑1 (MQ=255)                                                                                                                     cATCTCCATCGCCATAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCAt                                                                       >  1:92893/1‑66 (MQ=255)                                                                                                                     cATCTCCATCGCCATAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCAt                                                                       <  2:92893/66‑1 (MQ=255)                                                                                                                           cATCGCCATAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCa                                                                  <  1:190894/65‑1 (MQ=255)                                                                                                                           cATCGCCATAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCa                                                                  >  2:190894/1‑65 (MQ=255)                                                                                                                                   |                                                                                                                         TCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCGCCCATCAACATGCTAAAACCGCCATCGCCGCACATGGCGACCACCTG  >  NC_000913/909524‑909774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑