Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	919,010	A→G	S37S (AGT→AGC)	ybjX ←	DUF535 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	919,010	0	A	G	100.0%	167.5 / NA	47	S37S (AGT→AGC)	ybjX	DUF535 family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/24);  total (23/24)

GAGATTAGGCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAAT  >  NC_000913/918875‑919144                                                                                                                                        |                                                                                                                                       gagaTTAGGCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGGCTa                                                                                                                                     >  1:850449/1‑139 (MQ=255)       aGGCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGGCTAAGaaaa                                                                                                                               >  1:1014562/1‑139 (MQ=255)       aGGCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGGCTAAGaaaa                                                                                                                               >  1:247421/1‑139 (MQ=255)              tGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGGCt                                                                                                                                      >  1:727107/1‑125 (MQ=255)              tGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGGCTAAg                                                                                                                                   <  1:968006/128‑1 (MQ=255)              tGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGGCTAAg                                                                                                                                   >  2:968006/1‑128 (MQ=255)                ggAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGGCTAAGAAAAAGTGACAgg                                                                                                                      <  1:954821/139‑1 (MQ=255)                        cgttcATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGGCTAAGAAAAAGTGACAGGCTGCTgag                                                                                                              >  1:1172672/1‑139 (MQ=255)                                       cGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGGCTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGt                                                                                               >  1:1084644/1‑139 (MQ=255)                                       cGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGGCTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGt                                                                                               >  2:187740/1‑139 (MQ=255)                                             aaCGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGGCTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGt                                                                                         <  1:907512/139‑1 (MQ=255)                                                              gAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCTGCCGACACTGTCCACGTGCCAGGCTAAGAAAAAGTGACAGGCTGCTGAGGGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCg                                                                        <  1:813631/139‑1 (MQ=255)                                                              gAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGGCTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCg                                                                        <  2:467068/139‑1 (MQ=255)                                                                 cgcAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGGCTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACa                                                                     >  2:685118/1‑139 (MQ=255)                                                                   cagcagAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGGCTAAGAAAAAGTGACa                                                                                                                        >  1:838366/1‑86 (MQ=255)                                                                   cagcaAAAATTTCTGGCGATAACTACGGCCATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGGCTAATAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTAGGTTAGCTGCGACata                                                                   >  2:813631/1‑139 (MQ=255)                                                                   cagcaAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGGCTAAGAAAAAGTGACa                                                                                                                        <  2:838366/86‑1 (MQ=255)                                                                   cagcaAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGGCTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACata                                                                   >  1:221996/1‑139 (MQ=255)                                                                   cagcaAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGGCTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACata                                                                   >  2:156427/1‑139 (MQ=255)                                                                        aaaaTTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGGCTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACATATTCAt                                                              <  2:1014562/139‑1 (MQ=255)                                                                         aaaTTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGGCTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACATATTCATg                                                             <  2:1084644/139‑1 (MQ=255)                                                                               cTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGGCTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACATATTCATGAAATCa                                                       <  1:1126894/139‑1 (MQ=255)                                                                               cTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGGCTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACATATTCATGAAATCa                                                       <  1:187740/139‑1 (MQ=255)                                                                                tGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGGCTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACATATTCATGAAATCaa                                                      <  2:721091/139‑1 (MQ=255)                                                                                tGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGGCTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACATATTCATGAAATCaa                                                      <  1:156427/139‑1 (MQ=255)                                                                                tGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGGCTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACATATTCATGAAATCaa                                                      <  2:850449/139‑1 (MQ=255)                                                                                            cGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGGCTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACATATTCATGAAATCAATGGTTATACATg                                          <  2:221996/139‑1 (MQ=255)                                                                                                gATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGGCTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGt                                                                                               >  2:732236/1‑82 (MQ=255)                                                                                                gATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGGCTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGt                                                                                               <  1:732236/82‑1 (MQ=255)                                                                                                  tGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGGCTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACATATTCATGAAATCaa                                                      >  1:174594/1‑121 (MQ=255)                                                                                                  tGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGGCTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACATATTCATGAAATCaa                                                      <  2:174594/121‑1 (MQ=255)                                                                                                   gCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGGCTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACATATTCATGAAATc                                                        >  1:643224/1‑118 (MQ=255)                                                                                                   gCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGGCTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACATATTCATGAAATc                                                        <  2:643224/118‑1 (MQ=255)                                                                                                    ccAGAATTTACCCGGCCGACACTGTCCACGTGCCAGGCTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACATATTCATGAAATCAATGGTTATACATGGCGTCGAt                                  >  1:680247/1‑139 (MQ=255)                                                                                                             aCCCGGCCGACACTGTCCACGTGCCAGGCTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCg                                                                                                <  1:668780/68‑1 (MQ=255)                                                                                                             aCCCGGCCGACACTGTCCACGTGCCAGGCTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCg                                                                                                >  2:668780/1‑68 (MQ=255)                                                                                                                  gCCGACACTGTCCACGTGCCAGGCTAAGAAAAAGTGAc                                                                                                                         >  2:1156955/1‑38 (MQ=255)                                                                                                                  gCCGACACTGTCCACGTGCCAGGCTAAGAAAAAGTGAc                                                                                                                         <  1:1156955/38‑1 (MQ=255)                                                                                                                           gTCCACGTGCCAGGCTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCa           <  2:1172672/139‑1 (MQ=255)                                                                                                                           gTCCACGTGCCAGGCTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCa           <  2:680247/139‑1 (MQ=255)                                                                                                                            tCCACGTGCCAGGCTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAgg                                                                                          >  2:201984/1‑59 (MQ=255)                                                                                                                            tCCACGTGCCAGGCTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAgg                                                                                          <  1:201984/59‑1 (MQ=255)                                                                                                                            tCCACGTGCCAGGCTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCaa          >  2:1219190/1‑139 (MQ=255)                                                                                                                             ccACGTGCCAGGCTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCaaa         >  1:1118183/1‑139 (MQ=255)                                                                                                                             ccACGTGCCAGGCTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCaaa         >  2:153958/1‑139 (MQ=255)                                                                                                                             ccACGTGCCAGGCTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCaaa         <  1:153958/139‑1 (MQ=255)                                                                                                                                    ccAGGCTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAAt  <  2:247421/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       GAGATTAGGCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAAT  >  NC_000913/918875‑919144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑