Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,154,109	G→A	*270* (TAG→TAA)	pabC →	4‑amino‑4‑deoxychorismate lyase component of para‑aminobenzoate synthase multienzyme complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,154,109	0	G	A	100.0%	140.4 / NA	44	*270* (TAG→TAA)	pabC	4‑amino‑4‑deoxychorismate lyase component of para‑aminobenzoate synthase multienzyme complex
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/21);  total (23/21)

TCGTTGCAGGCAGATGAGATGGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAGTCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGCCATCTTGCCGACAGCAAATTGCTTATCAAAGAAGAGACGATATT  >  NC_000913/1153975‑1154236                                                                                                                                       |                                                                                                                                tCGTTGCAGGCAGATGAGATGGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCAt                                                                                                                             >  2:1040534/1‑139 (MQ=255) tCGTTGCAGGCAGATGAGATGGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCAt                                                                                                                             >  2:1014583/1‑139 (MQ=255)        aggcagATGAGATGGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGaaaaaa                                                                                                                      <  1:1014583/139‑1 (MQ=255)                            tGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGtt                                                                                                  >  1:222316/1‑139 (MQ=255)                             gTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTa                                                                                                 <  2:923333/139‑1 (MQ=255)                                aTGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTa                                                                                                                >  2:485538/1‑121 (MQ=255)                                aTGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTa                                                                                                                <  1:485538/121‑1 (MQ=255)                                 tGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGc                                                                                             >  1:1031616/1‑139 (MQ=255)                                    gTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCtgg                                                                                          <  2:222316/139‑1 (MQ=255)                                     ttAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAGAAAGTGTTATTGATAATCTTGTTATTGc                                                                                             >  1:236700/1‑135 (MQ=255)                                     ttAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAGAAAGTGTTATTGATAATCTTGTTATTGc                                                                                             <  2:236700/135‑1 (MQ=255)                                                  tGCCCGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATc                                                                            >  1:578791/1‑139 (MQ=255)                                                    cccGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGaaaaaa                                                                                                                      >  2:882073/1‑95 (MQ=255)                                                    cccGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGaaaaaa                                                                                                                      <  1:882073/95‑1 (MQ=255)                                                         aTGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTg                                                                                              >  2:1207088/1‑114 (MQ=255)                                                         aTGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTg                                                                                              <  1:1207088/114‑1 (MQ=255)                                                             gCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGgtgt                                                                 >  1:859724/1‑139 (MQ=255)                                                             gCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGgtgt                                                                 >  2:386150/1‑139 (MQ=255)                                                              ccTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGa                                                                                                                           <  1:1045365/80‑1 (MQ=255)                                                              ccTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGa                                                                                                                           >  2:1045365/1‑80 (MQ=255)                                                              ccTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTg                                                                                              >  1:758037/1‑109 (MQ=255)                                                              ccTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTg                                                                                              <  2:758037/109‑1 (MQ=255)                                                              ccTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGtgtg                                                                <  2:1031616/139‑1 (MQ=255)                                                                 gtgGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGc                                                             <  2:962519/139‑1 (MQ=255)                                                                 gtgGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGc                                                             <  2:859724/139‑1 (MQ=255)                                                                       aTGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCtggtg                                                                                        >  1:891873/1‑106 (MQ=255)                                                                       aTGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCtggtg                                                                                        <  2:891873/106‑1 (MQ=255)                                                                                           aCGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAGGGTTCGCCATCTTGCCGa                                   <  2:801477/139‑1 (MQ=255)                                                                                           aCGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGCCATCTTGCCGa                                   <  2:1180958/139‑1 (MQ=255)                                                                                                 tatGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGCCATCTTGCCGACAGCaa                             >  2:639748/1‑139 (MQ=255)                                                                                                  atGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGCCATCTTGCCGACAGCaaa                            <  2:233450/139‑1 (MQ=255)                                                                                                  atGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGCCATCTTGCCGACAGCaaa                            <  1:655853/139‑1 (MQ=255)                                                                                                      atatTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGCCATCTTGCCGACAGCAAATTGc                        >  1:826402/1‑139 (MQ=255)                                                                                                      atatTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGCCATCTTGCCGACAGCAAATTGc                        >  1:58683/1‑139 (MQ=255)                                                                                                       tatTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGAt                                                                                                           <  1:1102781/55‑1 (MQ=255)                                                                                                       tatTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGAt                                                                                                           >  2:1102781/1‑55 (MQ=255)                                                                                                       tatTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGCCATCTTGCCGACAGCAAATTGCt                       >  1:551021/1‑139 (MQ=255)                                                                                                              ccccACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCtgg                                                                                          <  2:622256/65‑1 (MQ=255)                                                                                                              ccccACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCtgg                                                                                          >  1:622256/1‑65 (MQ=255)                                                                                                                 cACTTTGTGAGCGCCCGAATTAATCATGAAAAAAgtgt                                                                                                                  <  1:203737/38‑1 (MQ=255)                                                                                                                 cACTTTGTGAGCGCCCGAATTAATCATGAAAAAAgtgt                                                                                                                  >  2:203737/1‑38 (MQ=255)                                                                                                                  aCTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGCCATCTTGc                                      >  2:359754/1‑113 (MQ=255)                                                                                                                  aCTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGCCATCTTGc                                      <  1:359754/113‑1 (MQ=255)                                                                                                                            cgcCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGCCATCTTGCCGACAGCAAATTGCTTATCAAAGAAGAGACGATAtt  >  2:160126/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                TCGTTGCAGGCAGATGAGATGGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAGTCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGCCATCTTGCCGACAGCAAATTGCTTATCAAAGAAGAGACGATATT  >  NC_000913/1153975‑1154236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑