Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	117,798	A→G	L283P (CTA→CCA)	nadC ←	quinolinate phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	117,798	0	A	G	100.0%	164.9 / NA	46	L283P (CTA→CCA)	nadC	quinolinate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/18);  total (28/18)

AAGACAAGAAATTTGCGAGGCGTTACGAAGAAAGTTGGGGAAGGGGAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTAGCGCACCGACG‑‑GAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACGGCTTCGCGCATCTGTTCTGTTT  >  NC_000913/117665‑117930                                                                                                                                      |                                                                                                                                       aaGACAAGAAATTTGCGAGGCGTTACGAAGAAAGTTGGGGAAGGGGAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCa                                                                                                                                   <  1:33101/139‑1 (MQ=255)                                      gggAAGGGGAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACG‑‑GAGATAAAGTCCACGCCCGTTTCGGCAAAtt                                                                                            >  2:39333/1‑139 (MQ=255)                                           ggggAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACG‑‑GAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGc                                                                                       <  1:39333/139‑1 (MQ=255)                                             ggAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGAc                                                                                                                              <  1:848148/100‑1 (MQ=255)                                             ggAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGAc                                                                                                                              >  2:848148/1‑100 (MQ=255)                                                 aTTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACG‑‑GAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGtt                                                                                 >  1:183686/1‑139 (MQ=255)                                                      ccgcccgcGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACG‑‑GAGATAAAGTCCACGCTCGTTTCGGCAAATTCACGCAGTGTTTTGTc                                                                            >  1:139212/1‑139 (MQ=255)                                                        gcccgcGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACG‑‑GAGATAAAGTCCACGCCCGTTTCGGCa                                                                                                >  2:359932/1‑117 (MQ=255)                                                        gcccgcGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACG‑‑GAGATAAAGTCCACGCCCGTTTCGGCa                                                                                                <  1:359932/117‑1 (MQ=255)                                                          ccgcGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACG‑‑GAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTg                                                                        >  1:734712/1‑139 (MQ=255)                                                           cgcgATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACG‑‑GAGATAAAGTCCACGCCCGTTTCGGCa                                                                                                <  2:278231/114‑1 (MQ=255)                                                           cgcgATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACG‑‑GAGATAAAGTCCACGCCCGTTTCGGCa                                                                                                >  1:278231/1‑114 (MQ=255)                                                            gcgATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACG‑‑GAGATAAAGTCCACGCCCGTTTCGGCAAATTCACg                                                                                        <  2:804049/121‑1 (MQ=255)                                                            gcgATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACG‑‑GAGATAAAGTCCACGCCCGTTTCGGCAAATTCACg                                                                                        >  1:804049/1‑121 (MQ=255)                                                                          aaaTCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACG‑‑GAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACtt                                                        >  1:1110848/1‑139 (MQ=255)                                                                               tGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACG‑‑GAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGt                                                   >  2:528049/1‑139 (MQ=255)                                                                                      tATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACG‑‑GAGATAAAGTCCACGCCCGTTTCGGCAAATTCAc                                                                                         >  2:1136110/1‑94 (MQ=255)                                                                                      tATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACG‑‑GAGATAAAGTCCACGCCCGTTTCGGCAAATTCAc                                                                                         <  1:1136110/94‑1 (MQ=255)                                                                                           gCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGc                                         >  1:663488/1‑139 (MQ=255)                                                                                           gCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACG‑‑GAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACtt                                                        >  2:1212074/1‑122 (MQ=255)                                                                                           gCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACG‑‑GAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACtt                                                        <  1:1212074/122‑1 (MQ=255)                                                                                           gCGAAAACGCATTGAAAGGTCGAGTGATTGTACGTGTTTAGTTGGCGCACCGACG‑‑GAGATAAAGTCCACGCCCGTTTCGGCAAATTAACGCAGTGTTTTGTCAGTGAC‑TTGCCAGACACTTCCAGTAGCGCCTTGCCGt                                      >  1:897744/1‑139 (MQ=255)                                                                                                aaCGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGa                                                                                                                               >  2:248972/1‑48 (MQ=255)                                                                                                aaCGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGa                                                                                                                               <  1:248972/48‑1 (MQ=255)                                                                                                aaCGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACG‑‑GAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGt                                  >  2:796930/1‑139 (MQ=255)                                                                                                  cGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACG‑‑GAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGc                                >  1:944848/1‑139 (MQ=255)                                                                                                   gCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACG‑‑GAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCg                               <  2:944848/139‑1 (MQ=255)                                                                                                       tGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGa                                                                                                                               <  1:124717/41‑1 (MQ=255)                                                                                                       tGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGa                                                                                                                               >  2:124717/1‑41 (MQ=255)                                                                                                       tGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACG‑‑GAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTg                           >  1:369601/1‑139 (MQ=255)                                                                                                          aaGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACG‑‑GAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGAC‑TTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACgg                       <  2:897744/139‑1 (MQ=255)                                                                                                             gTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACG‑‑GAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACGGCt                     >  1:655263/1‑139 (MQ=255)                                                                                                             gTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACG‑‑GAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACGGCt                     >  1:497306/1‑139 (MQ=255)                                                                                                              tCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACG‑‑GAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACGGCtt                    <  1:528049/139‑1 (MQ=255)                                                                                                               cGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACG‑‑GAGATAAAGTCCACGCCCGTTTCGGCAAATTCACg                                                                                        >  1:655092/1‑70 (MQ=255)                                                                                                               cGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACG‑‑GAGATAAAGTCCACGCCCGTTTCGGCAAATTCACg                                                                                        <  2:655092/70‑1 (MQ=255)                                                                                                                gAGTGCTTGTACGTGTTTAGTTGGCGCACCGACG‑‑GAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACGACTTcg                  <  2:428380/139‑1 (MQ=255)                                                                                                                 aGTGCTTGTACGTGTTTAGTTGGCGCACCGACG‑‑GAGATAAAGTCCACGCCCGTTTCGGCa                                                                                                <  2:235494/60‑1 (MQ=255)                                                                                                                 aGTGCTTGTACGTGTTTAGTTGGCGCACCGACG‑‑GAGATAAAGTCCACGCCCGTTTCGGCa                                                                                                >  1:235494/1‑60 (MQ=255)                                                                                                                     cTTGTACGTGTTTAGTTGGCGCACCGACG‑‑GAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACGGCTTCGCGCAt             <  2:369601/139‑1 (MQ=255)                                                                                                                     cTTGTACGTGTTTAGTTGGCGCACCGACG‑‑GAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACGGCTTCGCGCAt             >  1:1181372/1‑139 (MQ=255)                                                                                                                        gTACGTGTTTAGTTGGCGCACCGACG‑‑GAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACGGCTTcg                  <  2:1205595/131‑1 (MQ=255)                                                                                                                        gTACGTGTTTAGTTGGCGCACCGACG‑‑GAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACGGCTTcg                  >  1:1205595/1‑131 (MQ=255)                                                                                                                            gtgtTTAGTTGGCGCACCGACG‑‑GAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACGGCTTCGCGCAtctgttct      >  1:561764/1‑139 (MQ=255)                                                                                                                               tttAGTTGGCGCACCGACG‑‑GAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACGGCTTCGCGCATctgttctgtt   >  1:596750/1‑139 (MQ=255)                                                                                                                               tttAGTTGGCGCACCGACG‑‑GAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACGGCTTCGCGCATctgttctgtt   >  1:739918/1‑139 (MQ=255)                                                                                                                                ttagttggCGCACCGACG‑‑GAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACGGCTTCGCGCATCTGTTCTGttt  <  2:739918/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                       AAGACAAGAAATTTGCGAGGCGTTACGAAGAAAGTTGGGGAAGGGGAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTAGCGCACCGACG‑‑GAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACGGCTTCGCGCATCTGTTCTGTTT  >  NC_000913/117665‑117930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑