Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,252,038	G→A	A325T (GCT→ACT)	dhaR →	dhaKLM operon transcription activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,252,038	0	G	A	100.0%	113.8 / NA	37	A325T (GCT→ACT)	dhaR	dhaKLM operon transcription activator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/16);  total (21/16)

GTGATAACCCTTAAACCGATAATAGAAACGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAGTCAGGCAATT  >  NC_000913/1251918‑1252169                                                                                                                         |                                                                                                                                    gTGATAACCCTTAAACCGATAATAGAAACGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACg                                                                                                                   <  2:24315/139‑1 (MQ=255)   gATAACCCTTAAACCGATAATAGAAACGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGAt                                                                                                                 >  1:1095676/1‑139 (MQ=255)    aTAACCCTTAAACCGATAATAGAAACGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATc                                                                                                                >  2:624660/1‑139 (MQ=255)            tAAACCGATAATAGAAACGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAAcc                                                                                                        >  1:108023/1‑139 (MQ=255)            tAAACCGATAATAGAAACGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAAcc                                                                                                        >  1:413368/1‑139 (MQ=255)                   ataataGAAACGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCt                                                                                                 >  1:586718/1‑139 (MQ=255)                   ataataGAAACGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGAAGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCt                                                                                                 >  1:712147/1‑139 (MQ=255)                          aaaCGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAgg                                                                                                                      <  2:768482/111‑1 (MQ=255)                          aaaCGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAgg                                                                                                                      >  1:768482/1‑111 (MQ=255)                          aaaCGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCa                                                                                          >  2:812075/1‑139 (MQ=255)                                aGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTgg                                                                                    >  1:591364/1‑139 (MQ=255)                                  ggAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTc                                                                                  >  2:866445/1‑139 (MQ=255)                                            ttATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAggcggc                                                                        >  2:620663/1‑139 (MQ=255)                                             tATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGgcggcg                                                                       <  1:620663/139‑1 (MQ=255)                                              aTTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTgg                                                                                    <  1:402581/125‑1 (MQ=255)                                              aTTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTgg                                                                                    >  2:402581/1‑125 (MQ=255)                                              aTTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGgcgc                                                                      >  1:482197/1‑139 (MQ=255)                                               ttttGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGgcgcg                                                                     <  2:1095676/139‑1 (MQ=255)                                                tttGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGgcgcgc                                                                    <  2:586718/139‑1 (MQ=255)                                                     tgCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAg                                                               <  2:413368/139‑1 (MQ=255)                                                                 tgGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTtcctgtcct                                                   >  1:1211630/1‑139 (MQ=255)                                                                 tgGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTtcctgtcct                                                   >  2:604557/1‑139 (MQ=255)                                                                 tgGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTtcctgtcct                                                   <  1:812075/139‑1 (MQ=255)                                                                 tgGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTtc                                                          <  2:277744/132‑1 (MQ=255)                                                                 tgGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTtc                                                          >  1:277744/1‑132 (MQ=255)                                                                  ggAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTtcctgtcctg                                                  <  2:108023/139‑1 (MQ=255)                                                                      cAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCttt                                              <  1:866445/139‑1 (MQ=255)                                                                                  ttGATGATCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCAGCtt                                                                                                <  1:709220/77‑1 (MQ=255)                                                                                  ttGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCtt                                                                                                >  2:709220/1‑77 (MQ=255)                                                                                             tCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAg                       >  1:909975/1‑139 (MQ=255)                                                                                              cAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAgg                      <  2:1211630/139‑1 (MQ=255)                                                                                              cAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAgg                      <  1:624660/139‑1 (MQ=255)                                                                                                         aGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAGt           >  1:612800/1‑139 (MQ=255)                                                                                                           tCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGAt                                                                                             >  2:411603/1‑55 (MQ=255)                                                                                                           tCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGAt                                                                                             <  1:411603/55‑1 (MQ=255)                                                                                                               ccATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAGTCAGGCa     >  2:414776/1‑139 (MQ=255)                                                                                                                  tACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAGTCAGGCAAtt  <  2:591364/139‑1 (MQ=255)                                                                                                                         |                                                                                                                                    GTGATAACCCTTAAACCGATAATAGAAACGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAGTCAGGCAATT  >  NC_000913/1251918‑1252169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑