Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,315,402	C→T	L123L (CTG→CTA)	yciF ←	putative rubrerythrin/ferritin‑like metal‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,315,402	0	C	T	100.0%	102.0 / NA	32	L123L (CTG→CTA)	yciF	putative rubrerythrin/ferritin‑like metal‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/16);  total (16/16)

ATTTCAGGCTTTATTTTCGGCTTTCTTATTTACGTTATTAATGGCCAGATCAGTCAGTTTGATGTCGGTGGCCTTTTCTTCTTCCAGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGGTAACCTAATTGTTCAGCCAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAATCAGTGCGGCATCACGCACTTCGTTTTTCTCGGTACTTTCGATGACCTCATTAGCTTCTTCAATAAGACCTTCC  >  NC_000913/1315267‑1315537                                                                                                                                        |                                                                                                                                        atTTCAGGCTTTATTTTCGGCTTTCTTATTTACGTTATTAATGGCCAGATCAGTCAGTTTGATGTCGGTGGCCTTTTCTTCTTCCAGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGGTAACCTAATTGTTcagctagc                                                                                                                                      <  1:40891/139‑1 (MQ=255)        gCTTTATTTTCGGCTTTCTTATTTACGTTATTAATGGCCAGATCAGTCAGTTTGATGTCGGTGGCCTTTTCTTCTTCCAGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTa                                                                                                                               >  1:231741/1‑139 (MQ=255)         cTTTATTTTCGGCTTTCTTATTTACGTTATTAATGGCCAGATCAGTCAGTTTGATGTCGGTGGCCTTTTCTTCTTCCAGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTaa                                                                                                                              <  2:66729/139‑1 (MQ=255)              ttttCGGCTTTCTTATTTACGTTATTAATGGCCAGATCAGTCAGTTTGATGTCGGTGGCCTTTTCTTCTTCCAGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTAATGTcc                                                                                                                         <  1:142599/139‑1 (MQ=255)                       ttCTTATTTACGTTATTAATGGCCAGATCAGTCAGTTTGATGTCGGTGGCCTTTTCTTCTTCCAGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTAATGTCCCGTAACTgg                                                                                                                <  1:799164/139‑1 (MQ=255)                        tCTTATTTACGTTATTAATGGCCAGATCAGTCAGTTTGATGTCGGTGGCCTTTTCTTCTTCCAGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTAATGTCCCGTAACTGGc                                                                                                               >  1:514749/1‑139 (MQ=255)                               tACGTTATTAATGGCCAGATCAGTCAGTTTGATGTCGGTGGCCTTTTCTTCTTCCAGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTAATGTCCCGTAACTGGCAATCTCa                                                                                                        <  2:231741/139‑1 (MQ=255)                                   ttattaATGGCCAGATCAGTCAGTTTGATGTCGGTGGCCTTTTCTTCTTCCAGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTa                                                                                                                               <  1:1153629/112‑1 (MQ=255)                                   ttattaATGGCCAGATCAGTCAGTTTGATGTCGGTGGCCTTTTCTTCTTCCAGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTa                                                                                                                               >  2:1153629/1‑112 (MQ=255)                                                      tcagtTTGATGTCGGTGGCCTTTTCTTCTTCCAGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGtgctgc                                                                                 >  1:476236/1‑139 (MQ=255)                                                           ttGATGTCGGTGGCCTTTTCTTCTTCCAGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCaa                                                                            >  1:939772/1‑139 (MQ=255)                                                            tGATGTCGGTGGCCTTTTCTTCTTCCAGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAAt                                                                           <  2:476236/139‑1 (MQ=255)                                                            tGATGTCGGTGGCCTTTTCTTCTTCCAGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAAt                                                                           <  2:514749/139‑1 (MQ=255)                                                                    gTGGCCTTTTCTTCTTCCAGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAATCAGTGCgg                                                                   >  2:345631/1‑139 (MQ=255)                                                                    gTGGCCTTTTCTTCTTCCAGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAATCAGTGCgg                                                                   >  1:894385/1‑139 (MQ=255)                                                                                      aGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTa                                                                                                                               >  2:947391/1‑61 (MQ=255)                                                                                      aGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTa                                                                                                                               <  1:947391/61‑1 (MQ=255)                                                                                       gggTTTCTTTCAGAAGCTTCGCTGCTTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAATCAGTGCGGCATCACGCACTTCGttttt                                                >  1:1075170/1‑139 (MQ=255)                                                                                            tctttcAGAAGCTTCGCTGCTTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTAATGTCCCGTAACTGGCAATCTCa                                                                                                        <  1:774495/78‑1 (MQ=255)                                                                                            tctttcAGAAGCTTCGCTGCTTTACGGTAACCTAATAGTTCAGCTAGAGTCGCTAATGTCCCGAAACTGGCAATCTCa                                                                                                        >  2:774495/1‑78 (MQ=255)                                                                                               ttcAGAAGCTTCGCTGCTTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAATCAGTGCGGCATCACGCACTTCGTTTTTCTCGGTAc                                        <  2:1075170/139‑1 (MQ=255)                                                                                                       cTTCGCTGCTTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTAATGTCCCGTAACTGGc                                                                                                               >  1:858436/1‑60 (MQ=255)                                                                                                       cTTCGCTGCTTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTAATGTCCCGTAACTGGc                                                                                                               <  2:858436/60‑1 (MQ=255)                                                                                                         tCGCTGCTTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAATCAGTGCGGCATCACGCACTTCGTTTTTCTCGGTACTTTCGATg                                <  2:36270/137‑1 (MQ=255)                                                                                                         tCGCTGCTTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAATCAGTGCGGCATCACGCACTTCGTTTTTCTCGGTACTTTCGATg                                >  1:36270/1‑137 (MQ=255)                                                                                                         tCGCTGCTTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAATCAGTGCGGCATCACGCACTTCGTTTTTCTCGGTACTTTCGATGAc                              >  2:1166679/1‑139 (MQ=255)                                                                                                             tgctTTACGGTAACCTAATTGTTCAGCTAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAATCAGTGCGGCATCACGCACTTCGTTTTTCTCGGTACTTTCGATGACCTCa                          <  2:123006/139‑1 (MQ=255)                                                                                                                  tACGGTAACCTAATTGTTCAGCTAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAATCAGTGCGGCATCACGCACTTCGTTTTTCTCGGTACTTTCGATGACCTCATTAGc                     <  1:690052/139‑1 (MQ=255)                                                                                                                         aCCTAATTGTTCAGCTAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAATCAGTGCGGCATCACGCACTTCGTTTTTCTCGGTACTTTCGATGACCTCATTAGCTTCTTCa              >  1:419018/1‑139 (MQ=255)                                                                                                                                 gTTCAGCTAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAAt                                                                           >  2:889055/1‑70 (MQ=255)                                                                                                                                 gTTCAGCTAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAAt                                                                           <  1:889055/70‑1 (MQ=255)                                                                                                                                 gTTCAGCTAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAATCAGTGCGGCATCACGc                                                           <  2:417782/86‑1 (MQ=255)                                                                                                                                 gTTCAGCTAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAATCAGTGCGGCATCACGc                                                           >  1:417782/1‑86 (MQ=255)                                                                                                                                     agctagcGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAATCAGTGCGGCATCACGCACTTCGTTTTtct                                              <  1:896068/95‑1 (MQ=255)                                                                                                                                     agctagcGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAATCAGTGCGGCATCACGCACTTCGTTTTTCTCGGTACTTTCGATGACCTCATTAGCTTCTTCAATAAGACCTTcc  >  1:493453/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                        ATTTCAGGCTTTATTTTCGGCTTTCTTATTTACGTTATTAATGGCCAGATCAGTCAGTTTGATGTCGGTGGCCTTTTCTTCTTCCAGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGGTAACCTAATTGTTCAGCCAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAATCAGTGCGGCATCACGCACTTCGTTTTTCTCGGTACTTTCGATGACCTCATTAGCTTCTTCAATAAGACCTTCC  >  NC_000913/1315267‑1315537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑