Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,366,935	C→T	*326* (TAG→TAA)	pspF ←	psp operon transcriptional activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,366,935	0	C	T	100.0%	209.0 / NA	65	*326* (TAG→TAA)	pspF	psp operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/29);  total (36/29)

CTGAGCGATTAATAGCCCAACGCAATAATGTCTGATGCGCTGCGCTTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGCTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAA  >  NC_000913/1366804‑1367069                                                                                                                                    |                                                                                                                                       cTGAGCGATTAATAGCCCAACGCAATAATGTCTGATGCGCTGCGCTTATCAGACCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCt                                                                                                                                 <  1:388578/139‑1 (MQ=255)      cGATTAATAGCCCAACGCAATAATGTCTGATGCGCTGCGCTTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGc                                                                                                                            <  2:262062/139‑1 (MQ=255)                     cGCAATAATGTCTGATGCGCTGCGCTTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAAcgcg                                                                                                             >  1:438281/1‑139 (MQ=255)                          taatGTCTGATGCGCTGCGCTTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGaa                                                                                                        >  1:403797/1‑139 (MQ=255)                                  gATGCGCTGCGCTTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACt                                                                                                      <  1:1160023/133‑1 (MQ=255)                                  gATGCGCTGCGCTTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACt                                                                                                      >  2:1160023/1‑133 (MQ=255)                                        ctgcgctTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTa                                                                                          >  1:1133856/1‑139 (MQ=255)                                        ctgcgctTATCAGGCCTGAAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTa                                                                                          >  1:676941/1‑139 (MQ=255)                                         tgcgctTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAAcgcg                                                                                                             >  1:162728/1‑119 (MQ=255)                                         tgcgctTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAAcgcg                                                                                                             <  2:162728/119‑1 (MQ=255)                                         tgcgctTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTaa                                                                                         >  2:441655/1‑139 (MQ=255)                                                     gCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTc                                                                             >  1:683328/1‑139 (MQ=255)                                                        tGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGc                                                                          >  1:1095326/1‑139 (MQ=255)                                                          cAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAAcgcg                                                                                                             <  1:573003/102‑1 (MQ=255)                                                          cAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAAcgcg                                                                                                             >  2:573003/1‑102 (MQ=255)                                                               gacgTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGtt                                                                                           >  2:1141309/1‑115 (MQ=255)                                                               gacgTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGtt                                                                                           <  1:1141309/115‑1 (MQ=255)                                                                acgTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCt                                                                  <  2:683328/139‑1 (MQ=255)                                                                    aTTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTg                                                              <  2:1133856/139‑1 (MQ=255)                                                                         ttATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGt                                                                                                                              <  1:422510/70‑1 (MQ=255)                                                                         ttATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGt                                                                                                                              >  2:422510/1‑70 (MQ=255)                                                                         ttATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAAcccc                                                                                     <  1:101405/111‑1 (MQ=255)                                                                         ttATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAAcccc                                                                                     >  2:101405/1‑111 (MQ=255)                                                                             gtatgCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATAttt                                                     >  1:274168/1‑139 (MQ=255)                                                                             gtatgCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATAttt                                                     >  1:833246/1‑139 (MQ=255)                                                                              tatgCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCcgcg                                                                      >  1:955708/1‑121 (MQ=255)                                                                              tatgCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCcgcg                                                                      <  2:955708/121‑1 (MQ=255)                                                                               atgCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTta                                                          <  1:830962/132‑1 (MQ=255)                                                                               atgCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTta                                                          >  2:830962/1‑132 (MQ=255)                                                                                tgCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTcccc                                                  >  2:786186/1‑139 (MQ=255)                                                                                tgCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTccc                                                  >  1:1217587/1‑139 (MQ=255)                                                                                tgCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAA‑CCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCt                                                 >  2:1087441/1‑139 (MQ=255)                                                                                 gCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCt                                                 >  2:529190/1‑139 (MQ=255)                                                                                  ccGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGtt                                                                                           <  2:717473/96‑1 (MQ=255)                                                                                  ccGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGtt                                                           >  1:356514/1‑128 (MQ=255)                                                                                  ccGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGtt                                                           <  2:356514/128‑1 (MQ=255)                                                                                  ccGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTccc                                                  >  1:1049554/1‑137 (MQ=255)                                                                                  ccGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTccc                                                  <  2:1049554/137‑1 (MQ=255)                                                                                  ccGAATAAAGCATTCACGCCACATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGtt                                                                                           >  1:717473/1‑96 (MQ=255)                                                                                   cGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCttg                                               >  1:83922/1‑139 (MQ=255)                                                                                     aaTAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCttgtt                                             >  2:585109/1‑139 (MQ=255)                                                                                      aTAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCttgtt                                             <  2:250569/138‑1 (MQ=255)                                                                                      aTAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCttgtt                                             >  1:250569/1‑138 (MQ=255)                                                                                        aaaGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTccc                                                  <  2:158401/131‑1 (MQ=255)                                                                                        aaaGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTccc                                                  >  1:158401/1‑131 (MQ=255)                                                                                            cATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAAcgcgcg                                                                                                           >  1:398282/1‑70 (MQ=255)                                                                                            cATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAAcgcgcg                                                                                                           <  2:398282/70‑1 (MQ=255)                                                                                               tCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACtgatg                                                                                                  <  1:92656/76‑1 (MQ=255)                                                                                               tCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACtgatg                                                                                                  >  2:92656/1‑76 (MQ=255)                                                                                                   gccgcATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTcaaca                                                                                                                  >  2:1047549/1‑56 (MQ=255)                                                                                                   gccgcATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTcaaca                                                                                                                  <  1:1047549/56‑1 (MQ=255)                                                                                                   gccgcATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAg                                                                                             >  1:845154/1‑77 (MQ=255)                                                                                                   gccgcATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAg                                                                                             <  2:845154/77‑1 (MQ=255)                                                                                                          ccGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAAct                        <  1:441655/139‑1 (MQ=255)                                                                                                                          cTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCaaa                                        >  1:75179/1‑107 (MQ=255)                                                                                                                          cTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCaaa                                        <  2:75179/107‑1 (MQ=255)                                                                                                                           tCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTgatgat                                                                                                <  1:912277/50‑1 (MQ=255)                                                                                                                           tCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTgatgat                                                                                                >  2:912277/1‑50 (MQ=255)                                                                                                                            cAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAATTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCt      <  2:1217587/139‑1 (MQ=255)                                                                                                                             aaCTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCtt                                                                 <  2:304429/79‑1 (MQ=255)                                                                                                                             aaCTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCtt                                                                 >  1:304429/1‑79 (MQ=255)                                                                                                                              aCTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGa    <  2:438281/139‑1 (MQ=255)                                                                                                                               cTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTatat                                                       <  1:766705/87‑1 (MQ=255)                                                                                                                               cTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTatat                                                       >  2:766705/1‑87 (MQ=255)                                                                                                                                ttCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGaaa  <  1:585109/139‑1 (MQ=255)                                                                                                                                    |                                                                                                                                       CTGAGCGATTAATAGCCCAACGCAATAATGTCTGATGCGCTGCGCTTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGCTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAA  >  NC_000913/1366804‑1367069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑