| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,389,895 | G→A | *322* (TAG→TAA) P235L (CCT→CTT) |
ycjG → mpaA ← |
L‑Ala‑D/L‑Glu epimerase murein peptide amidase A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,389,895 | 0 | G | A | 100.0% | 100.4 / NA | 32 | *322* (TAG→TAA) P235L (CCT→CTT) | ycjG mpaA | L‑Ala‑D/L‑Glu epimerase murein peptide amidase A |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (16/16); total (16/16) | |||||||||||
CTCGTGCCATTAGCGCCGCTTTACCGCTGGTGCCGCAGGTCAGTTTCGCCGATCTTGACGGACCGACCTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAGGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATC > NC_000913/1389768‑1389998 | ctcGTGCCATTAGCGCCGCTTTACCGCTGGTGCCGCAGGTCAGTTTCGCCGATCTTGACGGACCGACCTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAgcgc > 1:854438/1‑139 (MQ=255) gTGCCATTAGCGCCGCTTTACCGCTGGTGCCGCAGGTCAGTTTCGCCGATCTTGACGGACCGACCTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCgcagc > 2:912607/1‑139 (MQ=255) tAGCGCCGCTTTACCGCTGGTGCCGCAGGTCAGTTTCGCCGATCTTGACGGACCGACCTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGGCGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTg > 1:730933/1‑139 (MQ=255) cgccgcTTTACCGCTGGTGCCGCAGGTCAGTTTCGCCGATCTTGACGGACCGACCTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCa > 1:411532/1‑139 (MQ=255) gcTTTACCGCTGGTGCCGCAGGTCAGTTTCGCCGATCTTGACGGACCGACCTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGc > 2:1156655/1‑139 (MQ=255) cTTTACCGCTGGTGCCGCAGGTCAGTTTCGCCGATCTTGACGGACCGACCTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCa < 2:854438/139‑1 (MQ=255) gTGCCGCAGGTCAGTTTCGCCGATCTTGACGGACCGACCTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATAttt > 2:641996/1‑139 (MQ=255) cGCAGGTCAGTTTCGCCGATCTTGACGGACCGACCTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCg > 2:937259/1‑139 (MQ=255) gACGGACCGACCTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATggg < 1:1156655/139‑1 (MQ=255) gaccgaccTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGa < 2:411532/139‑1 (MQ=255) ccgaccTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCa > 1:1127930/1‑95 (MQ=255) ccgaccTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCa < 2:1127930/95‑1 (MQ=255) ccTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAActct > 1:378380/1‑139 (MQ=255) ctggctggCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTg < 1:937259/139‑1 (MQ=255) gctggcGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCgg < 2:844962/139‑1 (MQ=255) ctggcGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTATGCGGt > 1:254297/1‑139 (MQ=255) ggcGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCaa < 1:765841/85‑1 (MQ=255) ggcGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCaa > 2:765841/1‑85 (MQ=255) cGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGatg > 1:1026471/1‑139 (MQ=255) aTGTGGAACCGGCGCTTCAGTTCACGACTGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGatgcaatgc < 2:730933/139‑1 (MQ=255) aTGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGatgcaatgc < 2:378380/139‑1 (MQ=255) aTGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGatgcaatgc < 2:1026471/139‑1 (MQ=255) aTGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGAGCGGAAACTCTGCGGTGatgcaatgc < 1:641996/139‑1 (MQ=255) gagccGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGATGCAATGCAAGtt < 1:18261/136‑1 (MQ=255) ggCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGAt < 1:929268/139‑1 (MQ=255) gcgcTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATg < 2:679263/101‑1 (MQ=255) gcgcTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATg > 1:679263/1‑101 (MQ=255) gcgcTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATc > 1:418153/1‑139 (MQ=255) gacgGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCa > 1:438860/1‑46 (MQ=255) gacgGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCa < 2:438860/46‑1 (MQ=255) ttGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCg > 2:235775/1‑92 (MQ=255) ttGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCg < 1:235775/92‑1 (MQ=255) | CTCGTGCCATTAGCGCCGCTTTACCGCTGGTGCCGCAGGTCAGTTTCGCCGATCTTGACGGACCGACCTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAGGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATC > NC_000913/1389768‑1389998 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |