Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,421,933 T→C V272A (GTC→GCC)  ydaU → Rac prophage, conserved protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,421,9330TC100.0% 113.3 / NA 33V272A (GTC→GCC) ydaURac prophage, conserved protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/17);  total (16/17)

CGGGAGCTGGAAAAATTCCGCCAGCAGGGTTTTTCACCTGAGCAGGTGATTCGACACAGCATCGCCAATGAATACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGTCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAACGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCAT  >  NC_000913/1421797‑1422055
                                                                                                                                        |                                                                                                                          
cGGGAGCTGGAAAAATTCCGCCAGCAGGGTTTTTCACCTGAGCAGGTGATTCGACACAGCATCGCCAATGAATACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGcct                                                                                                                          <  1:917001/139‑1 (MQ=255)
       tGGAAAAATTCCGCCAGCAGGGTTTTTCACCTGAGCAGGTGATTCGACACAGCATCGCCAATGAATACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTAcc                                                                                                                   >  2:430683/1‑139 (MQ=255)
        ggAAAAATTCCGCCAGCAGGGTTTTTCACCTGAGCAGGTGATTCGACACAGCATCGCCAATGAATACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCg                                                                                                                  >  1:1005612/1‑139 (MQ=255)
                   gCCAGCAGGGTTTTTCACCTGAGCAGGTGATTCGACACAGCATCGCCAATGAATACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGcct                                                                                                                          >  1:459574/1‑120 (MQ=255)
                       gcagGGTTTTTCACCTGAGCAGGTGATTCGACACAGCATCGCCAATGAATACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCg                                                                                                   <  1:1020921/139‑1 (MQ=255)
                                 tCACCTGAGCAGGTGATTCGACACAGCATCGCCAATGAATACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCa                                                                                         <  2:1005612/139‑1 (MQ=255)
                                 tCACCTGAGCAGGTGATTCGACACAGCATCGCCAATGAATACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCa                                                                                         <  1:447274/139‑1 (MQ=255)
                                               gATTCGACACAGCATCGCCAATGAATACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGAcc                                                                           <  1:212022/139‑1 (MQ=255)
                                               gATTCGACACAGCATCGCCAATGAATACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGAcc                                                                           >  2:861859/1‑139 (MQ=255)
                                               gATTCGACACAGCATCGCCAATGAATACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGAcc                                                                           >  1:630831/1‑139 (MQ=255)
                                                 ttCGACACAGCATCGCCAATGAATACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCAt                                                                         <  1:861859/139‑1 (MQ=255)
                                                  tCGACACAGCATCGCCAATGAATACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATg                                                                        >  2:854450/1‑139 (MQ=255)
                                                  tCGACACAGCATCGCCAATGAATACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATg                                                                        >  2:913807/1‑139 (MQ=255)
                                                  tCGACACAGCATCGCCAATGAATACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATg                                                                        >  1:381523/1‑139 (MQ=255)
                                                             tCGCCAATGAATACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGc                                                             <  2:630831/139‑1 (MQ=255)
                                                                ccAATGAATACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGcct                                                                                                                          <  2:138350/75‑1 (MQ=255)
                                                                     gAATACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCg                                                     <  1:854450/139‑1 (MQ=255)
                                                                     gAATACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCg                                                     <  1:913807/139‑1 (MQ=255)
                                                                     gAATACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCg                                                     <  1:430683/139‑1 (MQ=255)
                                                                           cAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGaa                                                                      <  1:778463/116‑1 (MQ=255)
                                                                           cAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGaa                                                                      >  2:778463/1‑116 (MQ=255)
                                                                             gggCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGATACTCTGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGCGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCtt                                             >  2:815151/1‑139 (MQ=255)
                                                                                cTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAg                                                                                               >  2:318425/1‑86 (MQ=255)
                                                                                cTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAg                                                                                               <  1:318425/86‑1 (MQ=255)
                                                                                                     tCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTGCCGGATAGTGCGATCCCGCCAGGCtt                                                                                           >  2:795526/1‑69 (MQ=255)
                                                                                                     tCGACCTGAGACGTTACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCtt                                                                                           <  1:795526/69‑1 (MQ=255)
                                                                                                                   tACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCtt                                                                                           >  2:874450/1‑55 (MQ=255)
                                                                                                                   tACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCtt                                                                                           <  1:874450/55‑1 (MQ=255)
                                                                                                                   tACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGa                                                                             >  2:650526/1‑69 (MQ=255)
                                                                                                                   tACTCCGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGa                                                                             <  1:650526/69‑1 (MQ=255)
                                                                                                                       ccGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAACGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCa   <  2:381523/139‑1 (MQ=255)
                                                                                                                        cGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGa                                                    >  2:915409/1‑89 (MQ=255)
                                                                                                                        cGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGa                                                    <  1:915409/89‑1 (MQ=255)
                                                                                                                        cGACAGGTTAACACCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAACGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCAt  >  1:459183/1‑139 (MQ=255)
                                                                                                                                  acacCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAACGTATCCGCAGACTGGc                         <  2:404919/106‑1 (MQ=255)
                                                                                                                                  acacCGCCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAACGTATCCGCAGACTGGc                         >  1:404919/1‑106 (MQ=255)
                                                                                                                                        |                                                                                                                          
CGGGAGCTGGAAAAATTCCGCCAGCAGGGTTTTTCACCTGAGCAGGTGATTCGACACAGCATCGCCAATGAATACCAGGGCTTGTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGTCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAACGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCAT  >  NC_000913/1421797‑1422055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑