Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,497,308	A→G	E151E (GAA→GAG)	opgD →	OPG biosynthetic periplasmic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,497,308	0	A	G	100.0%	166.5 / NA	46	E151E (GAA→GAG)	opgD	OPG biosynthetic periplasmic protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/21);  total (25/21)

CCATATGGGAATGGGATTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGT  >  NC_000913/1497173‑1497434                                                                                                                                        |                                                                                                                               ccATATGGGAATGGGATTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAgggg                                                                                                                             <  1:279208/139‑1 (MQ=255)           atgggaTTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGAtc                                                                                                                   >  2:1135415/1‑139 (MQ=255)                 ttCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGc                                                                                                                            <  1:159933/124‑1 (MQ=255)                 ttCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGc                                                                                                                            >  2:159933/1‑124 (MQ=255)                   ccatCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCttt                                                                                                           >  1:706306/4‑139 (MQ=255)                            cgTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGtttt                                                                                                  >  1:987794/1‑139 (MQ=255)                                             tAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGttt                                                                                         <  1:68791/131‑1 (MQ=255)                                             tAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGttt                                                                                         >  2:68791/1‑131 (MQ=255)                                                        acacATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGTCCCCGAACTGgcgc                                                                      <  1:1048644/139‑1 (MQ=255)                                                          acaTCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGgcgcgc                                                                    >  2:82398/1‑139 (MQ=255)                                                           caTCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGcc                                                                   <  1:1135415/139‑1 (MQ=255)                                                            aTCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCg                                                                  >  2:15551/1‑139 (MQ=255)                                                            aTCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCg                                                                  >  1:685575/1‑139 (MQ=255)                                                             tCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGt                                                                 <  2:685575/139‑1 (MQ=255)                                                              cTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCg                                                                  <  2:469518/137‑1 (MQ=255)                                                              cTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCg                                                                  >  1:469518/1‑137 (MQ=255)                                                                 gcgcGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATg                                                             <  1:15551/139‑1 (MQ=255)                                                                      tGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCg                                                                  <  2:234547/129‑1 (MQ=255)                                                                      tGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCg                                                                  >  1:234547/1‑129 (MQ=255)                                                                      tGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATgtagta                                                        >  2:996737/1‑139 (MQ=255)                                                                                   cGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAg                                                                                                                        <  1:101085/62‑1 (MQ=255)                                                                                   cGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAg                                                                                                                        >  2:101085/1‑62 (MQ=255)                                                                                              gttCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTc                                >  1:949652/1‑139 (MQ=255)                                                                                                    aTACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGAtctc                                                                                                                 <  1:409626/52‑1 (MQ=255)                                                                                                    aTACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGAtctc                                                                                                                 >  2:409626/1‑52 (MQ=255)                                                                                                    aTACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCcgc                             >  1:484142/1‑136 (MQ=255)                                                                                                    aTACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCcgc                             <  2:484142/136‑1 (MQ=255)                                                                                                    aTACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGcc                          <  1:82398/139‑1 (MQ=255)                                                                                                    aTACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGcc                          <  2:949652/139‑1 (MQ=255)                                                                                                    aTACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGcc                          >  1:250543/1‑139 (MQ=255)                                                                                                     tACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCg                         <  2:250543/139‑1 (MQ=255)                                                                                                        aaCGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTtg                      <  1:996737/139‑1 (MQ=255)                                                                                                        aaCGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTtg                      >  1:362546/1‑139 (MQ=255)                                                                                                         aCGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGcc                                                                   <  1:7781/93‑1 (MQ=255)                                                                                                         aCGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGcc                                                                   >  2:7781/1‑93 (MQ=255)                                                                                                         aCGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCg                                                                  <  2:465699/94‑1 (MQ=255)                                                                                                         aCGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCg                                                                  >  1:465699/1‑94 (MQ=255)                                                                                                         aCGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATgt                                                            <  2:376597/100‑1 (MQ=255)                                                                                                         aCGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATgt                                                            >  1:376597/1‑100 (MQ=255)                                                                                                         aCGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTtga                     >  2:1082806/1‑139 (MQ=255)                                                                                                                      tGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGgcg                                                                       >  2:674848/1‑76 (MQ=255)                                                                                                                      tGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGgcg                                                                       <  1:674848/76‑1 (MQ=255)                                                                                                                       gATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTcgcg                                                                                              <  2:193656/52‑1 (MQ=255)                                                                                                                       gATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTcgcg                                                                                              >  1:193656/1‑52 (MQ=255)                                                                                                                       gATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTtga                     >  2:1066876/1‑125 (MQ=255)                                                                                                                       gATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTtga                     <  1:1066876/125‑1 (MQ=255)                                                                                                                            aaaaCAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGt  >  1:613041/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                               CCATATGGGAATGGGATTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGT  >  NC_000913/1497173‑1497434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑