Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,565,758	C→T	*461* (TAG→TAA)	dosC ←	diguanylate cyclase, cold‑ and stationary phase‑induced oxygen‑dependent biofilm regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,565,758	0	C	T	100.0%	203.5 / NA	63	*461* (TAG→TAA)	dosC	diguanylate cyclase, cold‑ and stationary phase‑induced oxygen‑dependent biofilm regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/34);  total (29/34)

CATCACTTCATCATTTTCGTTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTGAG  >  NC_000913/1565629‑1565893                                                                                                                                  |                                                                                                                                        cATCACTTCATCATTTTCGTTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTg                                                                                                                                >  1:455519/1‑139 (MQ=255) cATCACTTCATCATTTTCGTTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTg                                                                                                                                >  2:1206189/1‑139 (MQ=255)   tCACTTCATCATTTTCGTTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGc                                                                                                                              >  1:762829/1‑139 (MQ=255)      cTTCATCATTTTCGTTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCttt                                                                                                                           >  1:419392/1‑139 (MQ=255)         catcatTTTCGTTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCa                                                                                                                        <  2:419392/139‑1 (MQ=255)                   gTTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAaca                                                                                                              <  1:337037/139‑1 (MQ=255)                        ttaaCACCGCACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGtt                                                                                                         >  1:366692/1‑139 (MQ=255)                             acCGCACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTAc                                                                                                    >  2:154675/1‑139 (MQ=255)                                gCACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTc                                                                                                 >  2:398848/1‑139 (MQ=255)                                 cACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCg                                                                                                >  2:542501/1‑139 (MQ=255)                                 cACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCg                                                                                                <  1:613442/139‑1 (MQ=255)                                 cACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCg                                                                                                >  1:332435/1‑139 (MQ=255)                                      atcatATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCttt                                                                                           >  2:264047/1‑139 (MQ=255)                                      atcatATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCttt                                                                                           <  2:1191562/139‑1 (MQ=255)                                      atcatATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCttt                                                                                           <  1:1206189/139‑1 (MQ=255)                                      atcatATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCttt                                                                                           >  1:1191562/1‑139 (MQ=255)                                      atcatATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGATTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACATCGTCttt                                                                                           >  2:724407/1‑139 (MQ=255)                                        catATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTg                                                                                         <  2:455519/139‑1 (MQ=255)                                        catATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTg                                                                                         <  2:1001054/139‑1 (MQ=255)                                         atatTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTgg                                                                                        <  1:264047/139‑1 (MQ=255)                                             tttGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGat                                                                                    <  2:1082273/139‑1 (MQ=255)                                              ttGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCttt                                                                                           <  1:82045/131‑1 (MQ=255)                                              ttGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCttt                                                                                           >  2:82045/1‑131 (MQ=255)                                                gCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCCACACGGTTTCTACCTCGTCTTTTGGCGAtata                                                                                 <  1:724407/139‑1 (MQ=255)                                                gCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGAtata                                                                                 >  1:261109/1‑139 (MQ=255)                                                gCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGAtata                                                                                 <  2:238898/139‑1 (MQ=255)                                                 cTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGat                                                                                    <  1:14388/135‑1 (MQ=255)                                                 cTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGat                                                                                    >  2:14388/1‑135 (MQ=255)                                                  tCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACa                                                                               <  1:946705/139‑1 (MQ=255)                                                    aaGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGATTAGCTTCATGATTACCTCTGCATCCTGGCGTATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATATaga                                                                             <  1:791907/139‑1 (MQ=255)                                                     aGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACagag                                                                            >  1:154500/1‑139 (MQ=255)                                                      gggCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACgg                                                                                                           <  1:1004552/107‑1 (MQ=255)                                                      gggCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACgg                                                                                                           >  2:1004552/1‑107 (MQ=255)                                                           gggAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAaca                                                                                                              <  2:925930/99‑1 (MQ=255)                                                           gggAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAaca                                                                                                              >  1:925930/1‑99 (MQ=255)                                                            ggAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATc                                                                     <  2:154500/139‑1 (MQ=255)                                                              aaaaaaaTGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCgg                                                                   <  2:762829/139‑1 (MQ=255)                                                              aaaaaaaTGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCgg                                                                   <  2:332435/139‑1 (MQ=255)                                                                        cATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAAt                                                         >  2:1172437/1‑139 (MQ=255)                                                                                 cATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTc                                                <  2:366692/139‑1 (MQ=255)                                                                                        cGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCg                                                                                      >  1:148690/1‑94 (MQ=255)                                                                                        cGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCg                                                                                      <  2:148690/94‑1 (MQ=255)                                                                                             cGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATg                                    <  1:398848/139‑1 (MQ=255)                                                                                                   gCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCAtt                              <  1:542501/139‑1 (MQ=255)                                                                                                    cTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTg                                                                                         >  2:176447/1‑79 (MQ=255)                                                                                                    cTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTg                                                                                         <  1:176447/79‑1 (MQ=255)                                                                                                                ctctGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCa                 <  1:1172437/139‑1 (MQ=255)                                                                                                                  ctGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCAGCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCAcc               >  2:648940/1‑139 (MQ=255)                                                                                                                   tGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTAcc                                                                                                   >  1:616165/1‑54 (MQ=255)                                                                                                                   tGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTAcc                                                                                                   <  2:616165/54‑1 (MQ=255)                                                                                                                   tGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTgg                                                                                        <  1:876687/65‑1 (MQ=255)                                                                                                                   tGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTgg                                                                                        >  2:876687/1‑65 (MQ=255)                                                                                                                   tGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCg              >  1:920008/1‑139 (MQ=255)                                                                                                                     cATCCTGGCGCATTTAAAGTCTGGCTTTCCAGAGTTCAac                                                                                                               <  2:107474/40‑1 (MQ=255)                                                                                                                     cATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAac                                                                                                               >  1:107474/1‑40 (MQ=255)                                                                                                                       tCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGAt                                     <  1:103734/112‑1 (MQ=255)                                                                                                                       tCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGAt                                     >  2:103734/1‑112 (MQ=255)                                                                                                                             cgcATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAgg                                                     >  2:639877/1‑90 (MQ=255)                                                                                                                             cgcATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAgg                                                     <  1:639877/90‑1 (MQ=255)                                                                                                                              gcATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTga   <  1:240477/139‑1 (MQ=255)                                                                                                                               cATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTgag  <  2:261109/139‑1 (MQ=255)                                                                                                                               cATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTgag  <  1:648940/139‑1 (MQ=255)                                                                                                                               cATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTgag  >  2:98171/1‑139 (MQ=255)                                                                                                                                  |                                                                                                                                        CATCACTTCATCATTTTCGTTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTGAG  >  NC_000913/1565629‑1565893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑