Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,619,957	G→A	*128* (TAG→TAA)	marA →	multiple antibiotic resistance transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,619,957	0	G	A	100.0%	142.1 / NA	45	*128* (TAG→TAA)	marA	multiple antibiotic resistance transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/21);  total (24/21)

CTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTC‑TTTTCCGCGCAGGGCGTTGCGGAACAAACCACGCAGCCAGTTGTTACTTCTTGTGCCAATG  >  NC_000913/1619828‑1620091                                                                                                                                  |                                                                                                                                        cTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACg                                                                                                                                >  1:397582/1‑139 (MQ=255)     gAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGAc                                                                                                                            >  1:1138534/1‑139 (MQ=255)                   tctGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGc                                                                                                              <  1:236864/139‑1 (MQ=255)                     tGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCaa                                                                                                            >  1:821011/1‑139 (MQ=255)                        cccGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCa                                                                                                         >  1:301460/1‑139 (MQ=255)                                ttCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCa                                                                                                 <  1:999056/139‑1 (MQ=255)                                     aaaTTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTc                                                                                            <  2:821011/139‑1 (MQ=255)                                                   ttCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATagcag                                                                              >  2:87452/1‑139 (MQ=255)                                                      cgccgcATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTg                                                                           >  1:833457/1‑139 (MQ=255)                                                         cgcATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTgcgc                                                                        <  2:397582/139‑1 (MQ=255)                                                         cgcATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTgcgc                                                                        <  2:1138534/139‑1 (MQ=255)                                                              aaaTACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTAtt                                                                   <  1:636494/139‑1 (MQ=255)                                                                      gATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTC‑TTTTc                                                          >  2:370469/1‑139 (MQ=255)                                                                      gATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTC‑TTTTc                                                          >  2:235226/1‑139 (MQ=255)                                                                        tGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTC‑TTTTCcg                                                        >  2:687572/1‑139 (MQ=255)                                                                          aCCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAAt                                                                                   >  2:8452/1‑111 (MQ=255)                                                                          aCCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAAt                                                                                   <  1:8452/111‑1 (MQ=255)                                                                          aCCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTC‑TTTTCcgcg                                                      <  1:52577/139‑1 (MQ=255)                                                                              atatGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCtt                                                                      <  2:792815/120‑1 (MQ=255)                                                                              atatGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTAACAACGTCACTGAGGCAATCATGAAACCACTATCATCCGCAATAGCAGCTGCGCtt                                                                      >  1:792815/1‑120 (MQ=255)                                                                                      ggCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTC‑TTTTCCGCGCa                                                    >  2:1130122/1‑129 (MQ=255)                                                                                      ggCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTC‑TTTTCCGCGCa                                                    <  1:1130122/129‑1 (MQ=255)                                                                                           aTCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATtct                                                                 <  1:409211/112‑1 (MQ=255)                                                                                           aTCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATtct                                                                 >  2:409211/1‑112 (MQ=255)                                                                                           aTCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTTCCGCGCAGGGCGTTGCGGAAc                                      >  1:763780/1‑139 (MQ=255)                                                                                           aTCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTC‑TTTTCCGCGCAGGGCGTTGCGGAACa                                     >  2:518491/1‑139 (MQ=255)                                                                                                  tttttACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTC‑TTTTCCGCGCAGGGCGTTGCGGAACAAACCACg                              <  2:948780/139‑1 (MQ=255)                                                                                                     ttACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTC‑TTTTCCGCGCAGGGCGTTGCGGAACAAACCACGCAg                           <  1:235226/139‑1 (MQ=255)                                                                                                      tACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTTCCGCGCAGGGCGTTGCGGAACAAACCACGCAg                           <  2:763780/139‑1 (MQ=255)                                                                                                      tACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTC‑TTTTCCGCGCAGGGCGTTGCGGAACAAACCACGCAGc                          <  1:87452/139‑1 (MQ=255)                                                                                                           ccATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCGTGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTC‑TTTTCCGCGCAGGGCGTTGCGGAACAAACCACGCAGCCAgtt                     <  2:1033325/139‑1 (MQ=255)                                                                                                              ttAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCa                                                                                                             >  1:52174/1‑49 (MQ=255)                                                                                                              ttAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCa                                                                                                             <  2:52174/49‑1 (MQ=255)                                                                                                              ttAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTC‑TTTTCCGCGCAGGGCGTTGCGGAACAAACCACGCAGCCAgttgtt                  >  2:560754/1‑139 (MQ=255)                                                                                                                       tACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTC‑TTTTCCGCGCAGGGCGTTGCGGAACAAACCACGCAGCCAGTTGTTACTTCTtgt         >  1:1212358/1‑139 (MQ=255)                                                                                                                        acaacaGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTC‑TTTTCCGCGCAGGGCGTTGCGGAACAAACCACGCAGCCa                        <  1:776376/123‑1 (MQ=255)                                                                                                                        acaacaGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTC‑TTTTCCGCGCAGGGCGTTGCGGAACAAACCACGCAGCCa                        >  2:776376/1‑123 (MQ=255)                                                                                                                            caGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTC‑TTTTCCGCGCAGGGCGTTGCGGAACAAACCACGCAGCCAg                       >  1:22344/1‑120 (MQ=255)                                                                                                                            caGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTC‑TTTTCCGCGCAGGGCGTTGCGGAACAAACCACGCAGCCAg                       <  2:22344/120‑1 (MQ=255)                                                                                                                            caGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTC‑TTTTCCGCGCAGGGCGTTGCGGAACAAACCACGCAGCCAGTTGTTACTTCTTGTGCCaa    <  1:560754/139‑1 (MQ=255)                                                                                                                             aGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTC‑TTTTCCGCGCAGGGCGTTGCGGAACAAACCACGCAGCCAGTTGTTACTTCTTGTGCCAAt   >  2:252053/1‑139 (MQ=255)                                                                                                                              gCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATtct                                                                 <  2:1119089/77‑1 (MQ=255)                                                                                                                              gCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATtct                                                                 >  1:1119089/1‑77 (MQ=255)                                                                                                                              gCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTC‑TTTTCCGCGCAGGGCGTTGCGGAACAAACCACGCAGCCAGTTGTTACTTCTTGTGCCAATg  >  2:946345/1‑139 (MQ=255)                                                                                                                              gCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTC‑TTTTCCGCGCAGGGCGTTGCGGAACAAACCACGCAGCCAGTTGTTACTTCTTGTGCCAATg  >  1:942309/1‑139 (MQ=255)                                                                                                                                  |                                                                                                                                        CTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTC‑TTTTCCGCGCAGGGCGTTGCGGAACAAACCACGCAGCCAGTTGTTACTTCTTGTGCCAATG  >  NC_000913/1619828‑1620091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑