Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,655,057	T→C	T29A (ACG→GCG)	rspA ←	bifunctional D‑altronate/D‑mannonate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,655,057	0	T	C	100.0%	182.1 / NA	51	T29A (ACG→GCG)	rspA	bifunctional D‑altronate/D‑mannonate dehydratase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/25);  total (26/25)

CTTTATAGAAAAACTGCCAGATATCTTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGTAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAAC  >  NC_000913/1654925‑1655188                                                                                                                                     |                                                                                                                                    cTTTATAGAAAAACTGCCAGATATCTTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATAcc                                                                                                                               >  1:468885/1‑139 (MQ=255) cTTTATAGAAAAACTGCCAGATATCTTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATAcc                                                                                                                               >  2:1159465/1‑139 (MQ=255)     ataGAAAAACTGCCAGATATCTTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTcc                                                                                                                           <  2:468885/139‑1 (MQ=255)      taGAAAAACTGCCAGATATCTTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCt                                                                                                                          <  1:70037/139‑1 (MQ=255)         aaaaaCTGCCAGATATCTTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAg                                                                                                                       <  2:38046/139‑1 (MQ=255)           aaaCTGCCAGATATCTTCGATACGGTGCGCATCGTGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAgtg                                                                                                                     >  2:279375/1‑139 (MQ=255)           aaaCTGCCAGATATCTTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAg                                                                                                                       >  1:1057640/1‑137 (MQ=255)           aaaCTGCCAGATATCTTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAg                                                                                                                       <  2:1057640/137‑1 (MQ=255)              cTGCCAGATATCTTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAgtggtg                                                                                                                  <  2:476982/139‑1 (MQ=255)                                  ggTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAAtt                                                                                              <  2:1214247/139‑1 (MQ=255)                                    tGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAAtt                                                                                              <  2:579820/137‑1 (MQ=255)                                    tGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAAtt                                                                                              >  1:579820/1‑137 (MQ=255)                                      cgcATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGc                                                                                          >  1:253285/1‑139 (MQ=255)                                       gcATCGCGACCATTAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGcc                                                                                         <  1:832743/139‑1 (MQ=255)                                         aTCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGcccc                                                                                       >  1:121591/1‑139 (MQ=255)                                         aTCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGcccc                                                                                       >  1:86405/1‑139 (MQ=255)                                           cgcgACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCgg                                                                                     <  1:1159465/139‑1 (MQ=255)                                            gcgACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGa                                                                                    <  2:393718/139‑1 (MQ=255)                                              gACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACa                                                                                  >  2:646421/1‑139 (MQ=255)                                              gACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACa                                                                                  >  2:467099/1‑139 (MQ=255)                                              gACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACa                                                                                  >  2:1026373/1‑139 (MQ=255)                                                          gCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAAc                                                                      >  1:251485/1‑139 (MQ=255)                                                           cGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACt                                                                     >  1:499872/1‑139 (MQ=255)                                                           cGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACt                                                                     >  2:559513/1‑139 (MQ=255)                                                            ggACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACtt                                                                    <  2:216635/139‑1 (MQ=255)                                                             gACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTc                                                                   <  1:646421/139‑1 (MQ=255)                                                                aaaGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGc                                                                <  1:279375/139‑1 (MQ=255)                                                                     tGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAAtt                                                                                              >  1:67073/1‑104 (MQ=255)                                                                     tGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAAtt                                                                                              <  2:67073/104‑1 (MQ=255)                                                                     tGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTa                                                           <  2:935186/139‑1 (MQ=255)                                                                       aTCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACg                                                         <  1:1026373/139‑1 (MQ=255)                                                                                       gCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAgtggt                                                                                                                   >  2:35468/1‑65 (MQ=255)                                                                                       gCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAgtggt                                                                                                                   <  1:35468/65‑1 (MQ=255)                                                                                            ggAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGa                                                                                    >  1:7040/1‑91 (MQ=255)                                                                                            ggAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGa                                                                                    <  2:7040/91‑1 (MQ=255)                                                                                               aaGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCAt                                 <  2:121591/139‑1 (MQ=255)                                                                                               aaGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCAt                                 <  2:86405/139‑1 (MQ=255)                                                                                                  cTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGc                              >  2:321363/1‑139 (MQ=255)                                                                                                          cATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCa                                                                                                                        <  2:45389/41‑1 (MQ=255)                                                                                                          cATTGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCa                                                                                                                        >  1:45389/1‑41 (MQ=255)                                                                                                            ttGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTg                           >  1:689114/1‑132 (MQ=255)                                                                                                            ttGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTg                           <  2:689114/132‑1 (MQ=255)                                                                                                             tGAGGGTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATg                   >  2:1096566/1‑139 (MQ=255)                                                                                                                 ggTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATg               >  2:1146152/1‑139 (MQ=255)                                                                                                                 ggTGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATg               >  2:638157/1‑139 (MQ=255)                                                                                                                   tcgCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGa                     <  1:836575/129‑1 (MQ=255)                                                                                                                   tGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGa                     >  2:836575/1‑131 (MQ=255)                                                                                                                   tGGCATCCCCAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGaa             >  1:464413/1‑139 (MQ=255)                                                                                                                           ccAAGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGc     <  2:499872/139‑1 (MQ=255)                                                                                                                             aaGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCaa   >  1:565353/1‑139 (MQ=255)                                                                                                                              aGGCCCGCAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAAc  <  1:1146152/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                    CTTTATAGAAAAACTGCCAGATATCTTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGTAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAAC  >  NC_000913/1654925‑1655188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑