Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,672,863 A→G L96P (CTG→CCG)  mdtI ← multidrug efflux system transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,672,8630AG100.0% 114.2 / NA 32L96P (CTG→CCG) mdtImultidrug efflux system transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/15);  total (17/15)

TTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGCCCCACAATGCATAAGCGACAGACAAGTCGATCCCTTTAACGGCTTGAGAA  >  NC_000913/1672732‑1672994
                                                                                                                                   |                                                                                                                                   
tttCTGTTACCTGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAAAAACGTTCAAACGCTGCCCAACAGCGCGGGCCGCGTCTTCATCAGGCAAGTTTCCCCATGATCATTCCAGCCAACAGCAAGACCGGGCCAAt                                                                                                                              <  1:952022/139‑1 (MQ=255)
                                  aCTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAAc                                                                                               <  2:239850/136‑1 (MQ=255)
                                  aCTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAAc                                                                                               >  1:239850/1‑136 (MQ=255)
                                   cTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCGGGc                                                                                                                                  >  1:949117/1‑100 (MQ=255)
                                   cTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCGGGc                                                                                                                                  <  2:949117/100‑1 (MQ=255)
                                        gCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCaa                                                                                      >  1:110268/1‑139 (MQ=255)
                                                    ttCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCtt                                                                                                                      >  1:412651/1‑95 (MQ=255)
                                                    ttCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCtt                                                                                                                      <  2:412651/95‑1 (MQ=255)
                                                      cAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGt                                                                        >  2:918494/1‑139 (MQ=255)
                                                       aaaCGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTg                                                                       <  2:110268/139‑1 (MQ=255)
                                                            cTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTggcggc                                                                  <  1:918494/139‑1 (MQ=255)
                                                              gCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCtt                                                                                                                      <  2:1174137/85‑1 (MQ=255)
                                                              gCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCtt                                                                                                                      >  1:1174137/1‑85 (MQ=255)
                                                              gCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCaa                                                                >  1:1059677/1‑139 (MQ=255)
                                                               cccGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTggcggc                                                                  >  2:477545/1‑136 (MQ=255)
                                                               cccGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTggcggc                                                                  <  1:477545/136‑1 (MQ=255)
                                                                       cgcgGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAAcc                                                       >  2:60040/1‑139 (MQ=255)
                                                                                                 tttCACCATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAAcc                                                                                    >  1:19270/1‑84 (MQ=255)
                                                                                                 tttCACCATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAAcc                                                                                    <  2:19270/84‑1 (MQ=255)
                                                                                                           aTCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGAc                                                                                                    >  2:516511/1‑58 (MQ=255)
                                                                                                           aTCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGAc                                                                                                    <  1:516511/58‑1 (MQ=255)
                                                                                                           aTCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGCCCCACAATGCATAAGCGACAGACAAGTCGATcc                   >  1:617682/1‑139 (MQ=255)
                                                                                                               ttCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAAc                                                                                     >  2:1047734/1‑69 (MQ=255)
                                                                                                               ttCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAAc                                                                                     <  1:1047734/69‑1 (MQ=255)
                                                                                                               ttCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCaa                                                          >  1:911832/1‑96 (MQ=255)
                                                                                                               ttCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCaa                                                          <  2:911832/96‑1 (MQ=255)
                                                                                                               ttCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGCCCCACAATGCATAAGCgacaga                              >  2:255829/1‑124 (MQ=255)
                                                                                                               ttCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGCCCCACAATGCATAAGCgacaga                              <  1:255829/124‑1 (MQ=255)
                                                                                                               ttCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGCCCCACAATGCATAAGCGACAGACAAGTCGATCCCttt               <  1:60040/139‑1 (MQ=255)
                                                                                                                    gCCAACAGCAAGACCGGGCAAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCAGCTAACGTGGCGGCAATACCAAACCCGCCCCACAATGCATAAGCGACAGACAAGTCGATCCCTTTAACgg          >  1:915591/1‑139 (MQ=255)
                                                                                                                           gCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGCCCCACAATGCATAAGCGACAGACAAGTCGATCCCTTTAACGGCTTgaga   <  2:1059677/139‑1 (MQ=255)
                                                                                                                            cAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGCCCCACAATGCATAAGCGACAGACAAGTCGATCCCTTTAACGGCTTGAGaa  >  1:470111/1‑139 (MQ=255)
                                                                                                                                   |                                                                                                                                   
TTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGCCCCACAATGCATAAGCGACAGACAAGTCGATCCCTTTAACGGCTTGAGAA  >  NC_000913/1672732‑1672994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: