Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,735,484	T→C	V36A (GTC→GCC)	sodB →	superoxide dismutase, Fe

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,735,484	0	T	C	100.0%	244.3 / NA	68	V36A (GTC→GCC)	sodB	superoxide dismutase, Fe
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/33);  total (35/33)

GCAAATTAATAATAAAGGAGAGTAGCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACGCAGCTCAGGTCTGGAACCATACTTTCTACTGGAACTGCCT  >  NC_000913/1735351‑1735619                                                                                                                                      |                                                                                                                                        gCAAATTAATAATAAAGGAGAGTAGCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTa                                                                                                                                    >  1:1096687/1‑139 (MQ=255) gCAAATTAATAATAAAGGAGAGTAGCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTa                                                                                                                                    >  2:493533/1‑139 (MQ=255) gCAAATTAATAATAAAGGAGAGTAGCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGAACCATCAGACTTATGCCACTa                                                                                                                                    >  2:715870/1‑139 (MQ=255)             taaAGGAGAGTAGCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAAcc                                                                                                                        <  2:1082437/139‑1 (MQ=255)               aaGGAGAGTAGCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTg                                                                                                                      <  1:901723/139‑1 (MQ=255)               aaGGAGAGTAGCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTg                                                                                                                      <  1:611831/139‑1 (MQ=255)                aGGAGAGTAGCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCa                                                                                                                                       <  2:1094274/121‑1 (MQ=255)                   agagTAGCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTa                                                                                                                  <  2:1096687/139‑1 (MQ=255)                    gagTAGCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCTGACTTATGCCACTAACCTGAACAACCTGATTaa                                                                                                                 >  1:884439/1‑139 (MQ=255)                     agTAGCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTaaa                                                                                                                >  2:51696/1‑139 (MQ=255)                       tAGCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAgg                                                                                                              >  2:986541/1‑139 (MQ=255)                       tAGCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAgg                                                                                                              >  2:51809/1‑139 (MQ=255)                            aTGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACcg                                                                                                         >  2:933624/1‑139 (MQ=255)                                 aTTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCGCCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGttt                                                                                                    >  2:816511/1‑139 (MQ=255)                                 aTTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTACAGGTACCGCGttt                                                                                                    <  1:816511/139‑1 (MQ=255)                                   tCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTaa                                                                                                                                   <  1:1032533/106‑1 (MQ=255)                                   tCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTaa                                                                                                                                   >  2:1032533/1‑106 (MQ=255)                                           ccTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAAt                                                                                          >  1:838365/1‑139 (MQ=255)                                                         tGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGaa                                                                                                                             >  1:1184310/1‑90 (MQ=255)                                                         tGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGaa                                                                                                                             <  2:1184310/90‑1 (MQ=255)                                                              aaGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCg                                                                       >  1:233511/1‑139 (MQ=255)                                                              aaGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCg                                                                       <  1:1140632/139‑1 (MQ=255)                                                              aaGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCg                                                                       <  1:829896/139‑1 (MQ=255)                                                              aaGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCg                                                                       >  1:414395/1‑139 (MQ=255)                                                              aaGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCg                                                                       >  2:829896/1‑139 (MQ=255)                                                                   gCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTg                                                                                                   <  2:888982/106‑1 (MQ=255)                                                                   gCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTg                                                                                                   >  1:888982/1‑106 (MQ=255)                                                                   gCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGct                                                                  >  2:908432/1‑139 (MQ=255)                                                                    ctctGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGctc                                                                 >  2:138950/1‑139 (MQ=255)                                                                      ctGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTg                                                               >  1:1106448/1‑139 (MQ=255)                                                                         gCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAg                                                            >  2:284986/1‑139 (MQ=255)                                                                         gCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAg                                                            <  2:838365/139‑1 (MQ=255)                                                                         gCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAg                                                            <  1:51809/139‑1 (MQ=255)                                                                         gCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAg                                                            <  2:1106448/139‑1 (MQ=255)                                                                            ccGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTg                                                         <  2:233511/139‑1 (MQ=255)                                                                               cacaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAAcc                                                                                                                        <  2:483308/73‑1 (MQ=255)                                                                               cacaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAAcc                                                                                                                        >  1:483308/1‑73 (MQ=255)                                                                               cacaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCa                                                                     <  2:571694/124‑1 (MQ=255)                                                                               cacaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCg                                                      >  1:107039/1‑139 (MQ=255)                                                                               cacaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTGGAAGGTAAATCACTGGAAGAGATTATTCGCa                                                                     >  1:571694/1‑124 (MQ=255)                                                                                 caTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTa                                                    <  1:908432/139‑1 (MQ=255)                                                                                    ttCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTc                                                 >  1:385173/1‑139 (MQ=255)                                                                                         cGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTcaacaa                                            >  1:509321/1‑139 (MQ=255)                                                                                           gAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACg                                          <  1:284986/139‑1 (MQ=255)                                                                                                 aTCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACGCAGCTc                                    <  1:986541/139‑1 (MQ=255)                                                                                                      gTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACGCAGCTCAGGTc                               <  2:884439/139‑1 (MQ=255)                                                                                                          cACTACGGCAAGCACCATCAGACTTATGCCACTAAc                                                                                                                                  >  1:773980/1‑36 (MQ=255)                                                                                                          cACTACGGCAAGCACCATCAGACTTATGCCACTAAc                                                                                                                                  <  2:773980/36‑1 (MQ=255)                                                                                                             tACGGCAAGCACCATCAGACCTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACGCAGCTCAGGTCTGGAAcc                        <  1:1093379/139‑1 (MQ=255)                                                                                                               cGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTa                                                    <  1:1036232/109‑1 (MQ=255)                                                                                                               cGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTa                                                    >  2:1036232/1‑109 (MQ=255)                                                                                                                 gCAAGCACCATCAGACTTATGCCACTAACCTGaacaac                                                                                                                         <  1:452592/38‑1 (MQ=255)                                                                                                                 gCAAGCACCATCAGACTTATGCCACTAACCTGaacaac                                                                                                                         >  2:452592/1‑38 (MQ=255)                                                                                                                 gCAAGCACCATCAGACTTATGCCACTAACCTGaacaa                                                                                                                          <  2:55372/37‑1 (MQ=255)                                                                                                                 gCAAGCACCATCAGACTTATGCCACTAACCTGaacaa                                                                                                                          >  1:55372/1‑37 (MQ=255)                                                                                                                  cAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACGCAGCTCAGGTCTGGAACCATACt                   <  1:51696/139‑1 (MQ=255)                                                                                                                   acgCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACGCAGGTCAGGTCTGGAACCATACtt                  <  1:715870/137‑1 (MQ=255)                                                                                                                    aGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTg                                                         <  2:769029/99‑1 (MQ=255)                                                                                                                    aGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTg                                                         >  1:769029/1‑99 (MQ=255)                                                                                                                    aGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACGCAGCTCAGGTCTGGAACCATACttt                 >  2:952493/1‑139 (MQ=255)                                                                                                                      cACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACGCAGCTCAGGTCTGGAACCATACTTTCt               >  1:418501/1‑139 (MQ=255)                                                                                                                         cATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACGCAGCt                                     <  2:2848/114‑1 (MQ=255)                                                                                                                         cATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAAAGCAGCt                                     >  1:2848/1‑114 (MQ=255)                                                                                                                                 ttATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTa                                                    >  2:191861/1‑91 (MQ=255)                                                                                                                                 ttATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTa                                                    <  1:191861/91‑1 (MQ=255)                                                                                                                                  tATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACGCAGCTCAGGTCTGGAACCATACTTTCTACTGGAACTGcc   <  2:418501/139‑1 (MQ=255)                                                                                                                                   aTGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCa                                                                     <  1:755550/72‑1 (MQ=255)                                                                                                                                   aTGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACGCAGCTCAGGTCTGGAACCATACTTTCTACTGGAACTGCCt  >  1:309395/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                        GCAAATTAATAATAAAGGAGAGTAGCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACGCAGCTCAGGTCTGGAACCATACTTTCTACTGGAACTGCCT  >  NC_000913/1735351‑1735619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑