Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,756,152	T→C	L152P (CTG→CCG)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,756,152	0	T	C	100.0%	203.1 / NA	57	L152P (CTG→CCG)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/27);  total (30/27)

TCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCG  >  NC_000913/1756035‑1756287                                                                                                                      |                                                                                                                                        tCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCCGGGCGAAAACAAAGGTGTGaa                                                                                                                    <  1:918331/139‑1 (MQ=255)          aCCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCCGGGCGaa                                                                                                                                  >  1:1042794/1‑116 (MQ=255)          aCCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCCGGGCGaa                                                                                                                                  <  2:1042794/116‑1 (MQ=255)          aCCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCCGGGCGAAAACAAAGGTGTGAACCTGCCTgg                                                                                                           >  2:938879/1‑139 (MQ=255)           ccTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCCGGGCGAAAACAAAGGTGTGAACCTGCCTGGc                                                                                                          <  2:391719/139‑1 (MQ=255)                 tgtTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCCGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTcc                                                                                                    <  1:822100/139‑1 (MQ=255)                   ttGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCCGGACGAAAACAAAGATGTGAACCTGCCTGGCGTTTCCAt                                                                                                  >  2:682372/1‑139 (MQ=255)                       cAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCCGGACGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGct                                                                                              <  1:682372/139‑1 (MQ=255)                         acacCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCCGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGctct                                                                                            >  2:152959/1‑139 (MQ=255)                         acacCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCCGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGctct                                                                                            >  1:944479/1‑139 (MQ=255)                               tACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCCGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTGCATTGCTCTGCCAGc                                                                                      >  1:184091/1‑139 (MQ=255)                               tACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCCGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGc                                                                                      >  2:865122/1‑139 (MQ=255)                                aCTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCCGGGCGAAAACaa                                                                                                                             <  2:732299/99‑1 (MQ=255)                                aCTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCCGGGCGAAAACaa                                                                                                                             >  1:732299/1‑99 (MQ=255)                                    gTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCCGGGCGAAAACaa                                                                                                                             >  2:406469/1‑95 (MQ=255)                                    gTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCCGGGCGAAAACaa                                                                                                                             <  1:406469/95‑1 (MQ=255)                                     ttGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCCGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGc                                                                                >  1:611556/1‑139 (MQ=255)                                          gATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCCGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGaaa                                                                           <  2:944479/139‑1 (MQ=255)                                                 tGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCCGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACaa                                                                    >  1:154823/1‑139 (MQ=255)                                                   aTCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCCGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAAc                                                                  <  1:938879/139‑1 (MQ=255)                                                    tCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCCGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACa                                                                 <  1:865122/139‑1 (MQ=255)                                                        tATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCCGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGAc                                                             <  1:121987/139‑1 (MQ=255)                                                        tATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCCGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGAc                                                             >  1:142917/1‑139 (MQ=255)                                                         aTGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCCGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGAcc                                                            <  2:154823/139‑1 (MQ=255)                                                             aaGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCCGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGAt                                                        >  2:395625/1‑139 (MQ=255)                                                                  aCCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCCGGGCGa                                                                                                                                   <  1:1024837/59‑1 (MQ=255)                                                                  aCCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCCGGGCGa                                                                                                                                   >  2:1024837/1‑59 (MQ=255)                                                                  aCCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCCGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCa                                                                                     >  2:350557/1‑105 (MQ=255)                                                                  aCCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCCGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCa                                                                                     <  1:350557/105‑1 (MQ=255)                                                                  aCCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCCGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTg                                                   >  2:677093/1‑139 (MQ=255)                                                                  aCCGCCAGTTAAGGTAACGAAGTTATCTGTAAATTTATGAACAACGGTGATACGGGTGAAAACAAAGATGTGAACTTGCCTGGCGTTTCCATTGCTCTGCTAGCACTGGCTGAAATAAACAAACAGTTCCTGATATTTg                                                   >  1:104589/1‑139 (MQ=255)                                                                       cATTGTAGGTAACAAAGTTATATGTAAAGTGCTGAACAACGGTGACCCGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGt                                                                                                        >  1:1216130/1‑81 (MQ=255)                                                                       cATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCCGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGt                                                                                                        <  2:1216130/81‑1 (MQ=255)                                                                          tGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCCGGGCGAAAACa                                                                                                                              >  1:1114067/1‑56 (MQ=255)                                                                          tGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCCGGGCGAAAACa                                                                                                                              <  2:1114067/56‑1 (MQ=255)                                                                                 aaCAAAGTTATCTGTAAAGTGCTGAACATCGGTGACCCGGGCGAAAACa                                                                                                                              >  1:5045/1‑49 (MQ=255)                                                                                 aaCAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCCGGGCGAAAACa                                                                                                                              <  2:5045/49‑1 (MQ=255)                                                                                 aaCAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCCGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAAc                                                                  <  2:973353/109‑1 (MQ=255)                                                                                 aaCAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCCGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAAc                                                                  >  1:973353/1‑109 (MQ=255)                                                                                     aaGTTATCTGTAAAGTGCTGAACAACGGTGACCCGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGa                                <  2:184091/139‑1 (MQ=255)                                                                                        ttATCTGTAAAGTGCTGAACAACGGTGACCCGGGCGAAAACAAAGGTGTGAACCTGCCTGTCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGTGAACAAGGCGTa                                  <  1:135931/134‑1 (MQ=255)                                                                                        ttATCTGTAAAGTGCTGAACAACGGTGACCCGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGTGAACAAGGCGTa                                  >  2:135931/1‑134 (MQ=255)                                                                                          aTCTGTAAAGTGCTGAACAACGGTGACCCGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGa                                                                             >  1:641959/1‑89 (MQ=255)                                                                                          aTCTGTAAAGTGCTGAACAACGGTGACCCGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGa                                                                             <  2:641959/89‑1 (MQ=255)                                                                                              gTAAAGTGCTGAACAACGGTGACCCGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCa                                                                                     <  2:122210/77‑1 (MQ=255)                                                                                              gTAAAGTGCTGAACAACGGTGACCCGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCa                                                                                     >  1:122210/1‑77 (MQ=255)                                                                                                aaaGTGCTGAACAACGGTGACCCGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTtgctg                     <  2:142917/139‑1 (MQ=255)                                                                                                     gCTGAACAACGGTGACCCTGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTg                                                                                           >  1:678311/1‑64 (MQ=255)                                                                                                     gCTGAACAACGGTGACCCGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTg                                                                                           <  2:678311/64‑1 (MQ=255)                                                                                                         aacaacGGTGACCCGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAAc                                          >  2:1119095/1‑109 (MQ=255)                                                                                                         aacaacGGTGACCCGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAAc                                          <  1:1119095/109‑1 (MQ=255)                                                                                                         aacaacGCTGACCCGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACATGACCTGATCTTTGGATGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTa            >  1:36842/1‑139 (MQ=255)                                                                                                              cGGTGACCCGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGAc                                                             <  2:492456/85‑1 (MQ=255)                                                                                                              cGGTGACCCGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGAc                                                             >  1:492456/1‑85 (MQ=255)                                                                                                              cGGTGACCCGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCtt                  <  2:50988/128‑1 (MQ=255)                                                                                                              cGGTGACCCGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCtt                  >  1:50988/1‑128 (MQ=255)                                                                                                                   aCCCGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCg  >  2:160615/1‑139 (MQ=255)                                                                                                                      |                                                                                                                                        TCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCG  >  NC_000913/1756035‑1756287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑