Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 10,647 A→G V237A (GTC→GCC)  yaaW ← UPF0174 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091310,6470AG97.4% 130.8 / NA 39V237A (GTC→GCC) yaaWUPF0174 family protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (23/15);  total (24/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTACAGGGCCTCCCAGCCCCGCGCC  >  NC_000913/10515‑10780
                                                                                                                                    |                                                                                                                                     
cGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCTGTTTCCGGTATCAGGCCTGAg                                                                                                                                 <  1:1134784/139‑1 (MQ=255)
                   tttCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGgcg                                                                                                              <  2:171061/139‑1 (MQ=255)
                     tCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCgca                                                                                                            >  1:1002672/1‑139 (MQ=255)
                                cTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGAtt                                                                                                 <  1:150614/139‑1 (MQ=255)
                                     gtgtTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAg                                                                                            >  2:634626/1‑139 (MQ=255)
                                     gtgtTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAg                                                                                            >  2:134189/1‑139 (MQ=255)
                                     gtgtTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAg                                                                                            <  1:946973/139‑1 (MQ=255)
                                      tgtTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGt                                                                                           >  1:778441/1‑139 (MQ=255)
                                          ttCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACgg                                                                                       >  2:1117947/1‑139 (MQ=255)
                                           tCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGc                                                                                      >  1:688006/1‑139 (MQ=255)
                                                  gcgGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATc                                                                               >  2:404815/1‑139 (MQ=255)
                                                  gcgGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATc                                                                               >  2:247562/1‑139 (MQ=255)
                                                         aataatCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCAcgc                                                                        >  1:363159/1‑139 (MQ=255)
                                                                aGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTg                                                                                               <  1:757916/109‑1 (MQ=255)
                                                                aGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGgc                                                                 >  2:885690/1‑139 (MQ=255)
                                                                aGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGgc                                                                 >  1:9539/1‑139 (MQ=255)
                                                                aGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTg                                                                                               >  2:757916/1‑109 (MQ=255)
                                                                 gCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGgcg                                                                <  1:134189/139‑1 (MQ=255)
                                                                     gTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGgcg                                                                                                              <  1:827272/89‑1 (MQ=255)
                                                                     gTGAGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGgcg                                                                                                              >  2:827272/1‑89 (MQ=255)
                                                                                tCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGgcg                                                                                                              >  1:1143901/1‑78 (MQ=255)
                                                                                tCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGgcg                                                                                                              <  2:1143901/78‑1 (MQ=255)
                                                                                        cTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAAcccc                                         >  1:74155/1‑139 (MQ=255)
                                                                                          tttGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCtt                                                >  1:1118739/1‑130 (MQ=255)
                                                                                          tttGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCtt                                                <  2:1118739/130‑1 (MQ=255)
                                                                                               ggggAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGt                                  >  2:1218181/1‑139 (MQ=255)
                                                                                               ggggAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGt                                  <  2:1002672/139‑1 (MQ=255)
                                                                                               ggggAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGt                                  <  1:885690/139‑1 (MQ=255)
                                                                                                 ggAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGc                                >  2:746624/1‑139 (MQ=255)
                                                                                                   aaaatgaaaatTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGa                                                      >  2:1112142/1‑115 (MQ=255)
                                                                                                   aaaatgaaaatTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGa                                                      <  1:1112142/115‑1 (MQ=255)
                                                                                                   aaaatgaaaatTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCg                              >  1:512913/1‑139 (MQ=255)
                                                                                                    aaatgaaaatTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGc                             <  1:1218181/139‑1 (MQ=255)
                                                                                                     aatgaaaatTTTCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCa                            >  1:334556/1‑139 (MQ=255)
                                                                                                              tttCCCCGGTTTCCGGTATCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTACAggg                   <  1:404815/139‑1 (MQ=255)
                                                                                                                               aTCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTACAGGGCCTCCCAGCCccgcgcc  >  2:525550/1‑139 (MQ=255)
                                                                                                                               aTCAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTACAGGGCCTCCCAGCCccgcgcc  >  2:675666/1‑139 (MQ=255)
                                                                                                                                 cAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTACAggg                   <  2:1192628/120‑1 (MQ=255)
                                                                                                                                 cAGGCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTACAggg                   >  1:1192628/1‑120 (MQ=255)
                                                                                                                                    |                                                                                                                                     
CGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTACAGGGCCTCCCAGCCCCGCGCC  >  NC_000913/10515‑10780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: