Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,757,721 C→T *335* (TAG→TAA ldtE ← murein L,D‑transpeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,757,7210CT100.0% 96.9 / NA 31*335* (TAG→TAAldtEmurein L,D‑transpeptidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/14);  total (17/14)

CGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGC  >  NC_000913/1757603‑1757855
                                                                                                                      |                                                                                                                                      
cGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACat                                                                                                                    >  1:1217159/1‑139 (MQ=255)
cGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACat                                                                                                                    >  2:335312/1‑139 (MQ=255)
           cTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCT                                                                                                         <  1:775034‑M1/139‑1 (MQ=255)
                   cGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTcc                                                                                                               >  2:609699/1‑125 (MQ=255)
                   cGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTcc                                                                                                               <  1:609699/125‑1 (MQ=255)
                   cGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccatt                                                                                                 >  1:204644‑M1/1‑131 (MQ=255)
                        ggACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCt                                                                                                             >  2:602603/1‑122 (MQ=255)
                        ggACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCt                                                                                                             <  1:602603/122‑1 (MQ=255)
                             acaTGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccatcctgcgctgac                                                                                       <  2:204644‑M1/139‑19 (MQ=255)
                                      ctaAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaa                                                                              >  2:567120‑M1/1‑112 (MQ=255)
                                        aaaTACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaagg                                                                            >  1:892350‑M1/1‑110 (MQ=255)
                                                 aaGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTTCTtcaccattctgcgctgactctactgaaggcgcattgct                                                                   <  2:892350‑M1/139‑39 (MQ=255)
                                                             cTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagt                                                       >  2:492590‑M1/1‑89 (MQ=255)
                                                                       aaaaaTGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagttgctccactg                                             <  1:335312‑M1/139‑61 (MQ=255)
                                                                                        tgCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagttgctccactgctcac                                        >  1:615517‑M1/1‑62 (MQ=255)
                                                                                        tgCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagttgctccactgctcac                                        <  2:615517‑M1/127‑66 (MQ=255)
                                                                                          cgcCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagttgctccactgctcacggaaaccggatacc                          >  1:361448‑M1/1‑60 (MQ=255)
                                                                                          cgcCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagttgctccactgctcaccgaaaccggatacc                          >  2:222638‑M1/1‑60 (MQ=255)
                                                                                           gcCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagttgctccactgctcaccgaaaccggataccc                         <  1:492590‑M1/139‑81 (MQ=255)
                                                                                               ttttttttGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgct                                                                                          <  1:1030430‑M1/70‑16 (MQ=255)
                                                                                               ttttttttGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgct                                                                                          >  2:1030430‑M1/1‑55 (MQ=255)
                                                                                                tttttttGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACata                                                                                                                   <  1:1090507/44‑1 (MQ=255)
                                                                                                tttttttGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACata                                                                                                                   >  2:1090507/1‑44 (MQ=255)
                                                                                                  tttttGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgct                                                                                          >  1:742170‑M1/1‑52 (MQ=255)
                                                                                                  tttttGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgct                                                                                          <  2:742170‑M1/67‑16 (MQ=255)
                                                                                                  tttttGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattccgcgctgactctactgaaggcgcattgct                                                                   >  1:746675‑M1/1‑52 (MQ=255)
                                                                                                  tttttGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattccgcgctgactctactgaaggcgcattgct                                                                   <  2:746675‑M1/90‑39 (MQ=255)
                                                                                                          tGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtca                                                                                                      <  2:1001441‑M1/47‑4 (MQ=255)
                                                                                                          tGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtca                                                                                                      >  1:1001441‑M1/1‑44 (MQ=255)
                                                                                                            ctgttTACCGTTACTGCGTCGCGCGTAACATATTCCCTTGTTtcaccattctgcgctgactctactgaaggagcattgctggctgcggtagttgctccactgctcaccgaaaccggataccctgcccgacgatacaacg        <  1:304787‑M1/137‑98 (MQ=255)
                                                                                                                  aCCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagttgctccactgctcaccgaaaccggataccctgcccgacgatacaacgctttat  >  1:92945‑M1/1‑36 (MQ=255)
                                                                                                                  aCCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagttgctccactgctcaccgaaaccggataccctgcccgacgatacaacgctttat  <  1:567120‑M1/139‑104 (MQ=255)
                                                                                                                      |                                                                                                                                      
CGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGC  >  NC_000913/1757603‑1757855

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: