Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,765,668	T→C	V1006V (GTA→GTG)	ydiJ ←	putative FAD‑linked oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,765,668	0	T	C	100.0%	142.1 / NA	40	V1006V (GTA→GTG)	ydiJ	putative FAD‑linked oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/21);  total (19/21)

CAGGAACCCCACCATGTTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGTACCCCCGTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGCAGTCGCTGCATAGCCTGATGCCAGGATAACTCATAGATCCCGAGCGAATTTTCATGGTTT  >  NC_000913/1765561‑1765800                                                                                                            |                                                                                                                                     cAGGAACCCCACCATGTTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGCACCCCCGTGCCTTCAACCCGTTTTACCTGGc                                                                                                       <  2:1075635/139‑1 (MQ=255)   ggAACCCCAACATGTTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGCACCCCCGTGCCTTCAACCCGTTTTACCTGGCTa                                                                                                     >  2:817250/1‑139 (MQ=255)     aaCCCCACCATGTTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCACACCCCCGTGCCTTCAACCCGTTTTACCTGGCTACg                                                                                                   <  2:753101/139‑1 (MQ=255)           accaTGTTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGCACCCCCGTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGa                                                                                             >  2:199743/1‑139 (MQ=255)                 tttCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGCACCCCCGTGCCTTCAACCCGTTTTACCTgg                                                                                                        >  1:1031738/1‑122 (MQ=255)                 tttCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGCACCCCCGTGCCTTCAACCCGTTTTACCTgg                                                                                                        <  2:1031738/122‑1 (MQ=255)                                        tGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGCACCCCCGTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTAcgcg                                                                >  1:397441/1‑139 (MQ=255)                                         gCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGCACCCCCGTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCgg                                                               >  2:1084542/1‑139 (MQ=255)                                          cTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGCACCCCCGTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGc                                                              >  2:358951/1‑139 (MQ=255)                                           ttCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGCACCCCCGTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGCa                                                             >  2:27405/1‑139 (MQ=255)                                              cAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGCACCCCCGTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGCAGTc                                                          >  1:765009/1‑139 (MQ=255)                                              cAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGCACCCCCGTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGCAGTc                                                          <  2:397441/139‑1 (MQ=255)                                                    gATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGCACCCCCGTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGCAGTCGCTGCa                                                    >  2:414008/1‑139 (MQ=255)                                                    gATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGCACCCCCGTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGCAGTCGCTGCa                                                    <  1:116429/139‑1 (MQ=255)                                                     aTTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGCACCCCCGTGCCTTCAAc                                                                                                                     <  2:521378/73‑1 (MQ=255)                                                     aTTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGCACCCCCGTGCCTTCAAc                                                                                                                     >  1:521378/1‑73 (MQ=255)                                                     aTTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGCACCCCCGTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGa                                                                             <  2:101566/113‑1 (MQ=255)                                                     aTTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGCACCCCCGTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGa                                                                             >  1:101566/1‑113 (MQ=255)                                                     aTTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGCACCCCCGTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGCAGTCGCTGCAt                                                   >  1:963118/1‑139 (MQ=255)                                                     aTTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGCACCCCCGTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGCAGTCGCTGCAt                                                   <  1:27405/139‑1 (MQ=255)                                                        ttCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGCACCCCCGTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGCAGTCGCTGCATAGc                                                <  2:2517/139‑1 (MQ=255)                                                                   tatCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGCACCCCCGTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGCAGTCGCTGCATAGCCTGATGCCAgg                                     <  1:817250/139‑1 (MQ=255)                                                                   tatCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGCACCCCCGTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGCAGTCGCTGCATAGCCTGATGCCAgg                                     <  1:414008/139‑1 (MQ=255)                                                                     tCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGCACCCCCGTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGCAGTCGCTGCATAGCCTGATGCCAGGAt                                   <  1:1084542/139‑1 (MQ=255)                                                                     tCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGCACCCCCGTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGCAGTCGCTGCATAGCCTGATGCCAGGAt                                   <  2:963118/139‑1 (MQ=255)                                                                     tCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGCACCCCCGTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGCAGTCGCTGCATAGCCTGATGCCAGGAt                                   <  2:869472/139‑1 (MQ=255)                                                                     tCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGCACCCCCGTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGCAGTCGCTGCATAGCCTGATGCCAGGAt                                   <  2:309452/139‑1 (MQ=255)                                                                     tCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGCACCCCCGTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGCAGTCGCTGCATAGCCTGATGCCAGGAt                                   <  1:199743/139‑1 (MQ=255)                                                                        tttAATAATCTCCAGTAAAGCCTGCACAGGATGGCGCACCCCCGTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGCAGTc                                                          <  1:623643/113‑1 (MQ=255)                                                                        tttAATAATCTCCAGTAAAGCCTGCACAGGATGGCGCACCCCCGTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGCAGTc                                                          >  2:623643/1‑113 (MQ=255)                                                                                    cAGTAAAGCCTGCACAGGATGGCGCACCCCCGTGCCTTCAACCCGTTTTAc                                                                                                            <  2:468473/51‑1 (MQ=255)                                                                                    cAGTAAAGCCTGCACAGGATGGCGCACCCCCGTGCCTTCAACCCGTTTTAc                                                                                                            >  1:468473/1‑51 (MQ=255)                                                                                     aGTAAAGCCTGCACAGGATGGCGCACCCCCGTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGCAGTCGCTGCATAGCCTGATGCCAGGAt                                   >  2:329511/1‑123 (MQ=255)                                                                                     aGTAAAGCCTGCACAGGATGGCGCACCCCCGTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGCAGTCGCTGCATAGCCTGATGCCAGGAt                                   <  1:329511/123‑1 (MQ=255)                                                                                     aGTAAAGCCTGCACAGGATGGCGCACCCCCGTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGCAGTCGCTGCATAGCCTGATGCCAGGATAACTCATAGATCCCGa                   >  1:326798/1‑139 (MQ=255)                                                                                       tAAAGCCTGCACAGGATGGCGCACCCCCGTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGCAGTCGCTGCATAGCCTGATGCCAGGATAACTCATAGATCCCGAGc                 >  1:217934/1‑139 (MQ=255)                                                                                              tGCACAGGATGGCGCACCCCCGTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGCAGt                                                           <  2:488478/90‑1 (MQ=255)                                                                                              tGCACAGGATGGCGCACCCCCGTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGCAGt                                                           >  1:488478/1‑90 (MQ=255)                                                                                                      aTGGCGCACCCCCGTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGCAGTCGCTGCATAGCCTGATGCCAGGATAACTCATAGATCCCGAGCGAATTTTCATGGttt  >  1:1112614/1‑139 (MQ=255)                                                                                                      aTGGCGCACCCCCGTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGCAGTCGCTGCATAGCCTGATGCCAGGATAACTCATAGATCCCGAGCGAATTTTCATGGttt  <  2:600427/139‑1 (MQ=255)                                                                                                            |                                                                                                                                     CAGGAACCCCACCATGTTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTAAAGCCTGCACAGGATGGCGTACCCCCGTGCCTTCAACCCGTTTTACCTGGCTACGGCAGGAATATCCGGTCGCCAGACAGCGGTTACGCGGCAGTCGCTGCATAGCCTGATGCCAGGATAACTCATAGATCCCGAGCGAATTTTCATGGTTT  >  NC_000913/1765561‑1765800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑