Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,766,131 T→C N852S (AAC→AGC)  ydiJ ← putative FAD‑linked oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,766,1310TC100.0% 149.9 / NA 42N852S (AAC→AGC) ydiJputative FAD‑linked oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/19);  total (23/19)

CCAGTAAGACGTTAAACTCGCCACGCTCCTCGCCCAGCGCCAGTTTATATTCATCGCGATAACAAAGTACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGTTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTAATATGCTGGGCTTTGCCATTTGGCGAAAATGGCAGTAACACAGGCTGGAAACCTAATTTTTCGACCAGACGGACA  >  NC_000913/1766012‑1766262
                                                                                                                       |                                                                                                                                   
ccAGTAAGACGTTAAACTCGCCACGCTCCTCGCCCAGCGCCAGTTTATATTCATCGCGATAACAAAGTACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCtt                                                                                                                  >  2:233790/1‑139 (MQ=255)
ccAGTAAGACGTTAAACTCGCCACGCTCCTCGCCCAGCGCCAGTTTATATTCATCGCGATAACAAAGTACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCtt                                                                                                                  >  1:1163155/1‑139 (MQ=255)
         cGTTAAACTCGCCACGCTCCTCGCCCAGCGCCAGTTTATATTCATCGCGATAACAAAGTACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGt                                                                                                         <  1:1168852/139‑1 (MQ=255)
                 tCGCCACGCTCCTCGCCCAGCGCCAGTTTATATTCATCGCGATAACAAAGTACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCaa                                                                                                 >  1:864014/1‑139 (MQ=255)
                 tCGCCACGCTCCTCGCCCAGCGCCAGTTTATATTCATCGCGATAACAAAGTACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCaa                                                                                                 <  1:233790/139‑1 (MQ=255)
                                                    aTCGCGATAACAAAGTACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTAATATGCTGGGCTTTg                                                              >  2:562048/1‑139 (MQ=255)
                                                     tCGCGATAACAAAGTACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCt                                                                                                                   >  1:1025041/1‑85 (MQ=255)
                                                     tCGCGATAACAAAGTACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCt                                                                                                                   <  2:1025041/85‑1 (MQ=255)
                                                     tCGCGATAACAAAGTACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCtt                                                                                                      >  2:1094068/1‑98 (MQ=255)
                                                     tCGCGATAACAAAGTACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCtt                                                                                                      <  1:1094068/98‑1 (MQ=255)
                                                      cgcgATAACAAAGTACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAAt                                                                                                                            >  2:969622/1‑75 (MQ=255)
                                                      cgcgATAACAAAGTACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAAt                                                                                                                            <  1:969622/75‑1 (MQ=255)
                                                      cgcgATAACAAAGTACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTAATATGCTGGGCTTTGcc                                                            >  2:283313/1‑139 (MQ=255)
                                                      cgcgATAACAAAGTACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTAATATGCTGGGCTTTGcc                                                            >  2:572094/1‑139 (MQ=255)
                                                              cAAAGTACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGt                                                                                                         <  1:880078/86‑1 (MQ=255)
                                                              cAAAGTACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGt                                                                                                         >  2:880078/1‑86 (MQ=255)
                                                                 aGTACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTAATATGCTGGGCTTTGCCATTTGGCGaaa                                                 <  1:283313/139‑1 (MQ=255)
                                                                   tACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTAATATGCTGGGCTTTGCCATTTGGCGAAAAt                                               <  1:572094/139‑1 (MQ=255)
                                                                       aGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTAATATGCTGGGCTTTGCCATTTGGCGAAAAt                                               <  1:1069111/135‑1 (MQ=255)
                                                                       aGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTAATATGCTGGGCTTTGCCATTTGGCGAAAAt                                               >  2:1069111/1‑135 (MQ=255)
                                                                           ccGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTAATATGCTGGGCTTTGCCATTTGGCGAAAATGGCAGTaa                                       >  2:152415/1‑139 (MQ=255)
                                                                             ggATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTAATATGCTGGGCTTTGCCATTTGGCGAAAATGGCAGTAaca                                     >  1:728874/1‑139 (MQ=255)
                                                                                       ccaccaTTGGCATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTAATATGCTGGGCTTTGCCATTTGGCGAAAATGGCAGTAACACAGGCTGGaa                           <  2:1163155/139‑1 (MQ=255)
                                                                                                 cATACCCAGCTTCGCCATACGGCTGAGGAAATccgcc                                                                                                                       >  2:567656/1‑37 (MQ=255)
                                                                                                 cATACCCAGCTTCGCCATACGGCTGAGGAAATccgcc                                                                                                                       <  1:567656/37‑1 (MQ=255)
                                                                                                 cATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGaa                                                                                     <  1:214590/71‑1 (MQ=255)
                                                                                                 cATACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGaa                                                                                     >  2:214590/1‑71 (MQ=255)
                                                                                                   tACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTa                                                                             <  1:645082/77‑1 (MQ=255)
                                                                                                   tACCCAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTa                                                                             >  2:645082/1‑77 (MQ=255)
                                                                                                     cccAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGaaa                                                                                    >  2:1180814/1‑68 (MQ=255)
                                                                                                     cccAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGaaa                                                                                    <  1:1180814/68‑1 (MQ=255)
                                                                                                     cccAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGa                                                                                      <  1:620996/66‑1 (MQ=255)
                                                                                                     cccAGCTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGa                                                                                      >  2:620996/1‑66 (MQ=255)
                                                                                                          cTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTAATATGCTGGGCTTTGCCATTTGGCGaaa                                                 >  2:1215007/1‑98 (MQ=255)
                                                                                                          cTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTAATATGCTGGGCTTTGCCATTTGGCGaaa                                                 <  1:1215007/98‑1 (MQ=255)
                                                                                                          cTTCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTAATATGCTGGGCTTTGCCATTTGGCGAAAATGGCAGTAACACAGGCTGGAAACCTAATTTTTCGACCAGa        >  2:333081/1‑139 (MQ=255)
                                                                                                           ttCGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTAATATGCTGGGCTTTGCCATTTGGCGAAAATGGCAGTAACACAGGCTGGAAACCTAATTTTTCGACCAGAc       >  2:1171547/1‑139 (MQ=255)
                                                                                                             cGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGaaa                                                                                    >  2:1021396/1‑60 (MQ=255)
                                                                                                             cGCCATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGaaa                                                                                    <  1:1021396/60‑1 (MQ=255)
                                                                                                                cATACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTAATATGCTGGGCTTTGCCATTTGGCGAAAATGGCAGTAACACAGGCTGGAAACCTAATTTTTCGACCAGACGGACa  <  2:864014/139‑1 (MQ=255)
                                                                                                                  tACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGa                                                                                      <  2:118784/53‑1 (MQ=255)
                                                                                                                  tACGGCTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGa                                                                                      >  1:118784/1‑53 (MQ=255)
                                                                                                                       |                                                                                                                                   
CCAGTAAGACGTTAAACTCGCCACGCTCCTCGCCCAGCGCCAGTTTATATTCATCGCGATAACAAAGTACCAGCGCCGGATCGACGCCCACCATTGGCATACCCAGCTTCGCCATACGGTTGAGGAAATCCGCCGTCTTTTTCGCCGTCTTCGCAAAACGATTAAGAAAACCTTTAATATGCTGGGCTTTGCCATTTGGCGAAAATGGCAGTAACACAGGCTGGAAACCTAATTTTTCGACCAGACGGACA  >  NC_000913/1766012‑1766262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑