Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,862,634	A→G	intergenic (‑205/‑137)	msrB ← / → gapA	methionine sulfoxide reductase B/glyceraldehyde‑3‑phosphate dehydrogenase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,862,634	0	A	G	100.0%	168.7 / NA	47	intergenic (‑205/‑137)	msrB/gapA	methionine sulfoxide reductase B/glyceraldehyde‑3‑phosphate dehydrogenase A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/28);  total (19/28)

GGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGAGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAGGCAACCTTTTATTCACTAACAAATAGCTGG  >  NC_000913/1862498‑1862762                                                                                                                                         |                                                                                                                                 ggCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTgggg                                                                                                                                >  1:524479/1‑139 (MQ=255)         tttGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGagtcag                                                                                                                        <  2:116158/139‑1 (MQ=255)            gTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTcg                                                                                                                     >  2:913523/1‑139 (MQ=255)             tCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTcgc                                                                                                                    >  1:1158493/1‑139 (MQ=255)               cTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGt                                                                                                                  <  2:1211581/139‑1 (MQ=255)               cTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGt                                                                                                                  <  1:1153605/139‑1 (MQ=255)                          ctcGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGc                                                                                                       <  1:186470/139‑1 (MQ=255)                             gacgaAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGagtcag                                                                                                                        <  1:392502/119‑1 (MQ=255)                             gacgaAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGagtcag                                                                                                                        >  2:392502/1‑119 (MQ=255)                             gacgaAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGca                                                                                                      <  2:861733/137‑1 (MQ=255)                             gacgaAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTAGCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGca                                                                                                      >  1:861733/1‑137 (MQ=255)                                  aaTGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGagtcag                                                                                                                        >  2:456985/1‑114 (MQ=255)                                  aaTGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGagtcag                                                                                                                        <  1:456985/114‑1 (MQ=255)                                       cTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGAttt                                                                                          >  2:1061608/1‑139 (MQ=255)                                         gCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGt                                                                                        <  1:1103900/139‑1 (MQ=255)                                           aCCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCg                                                                                      <  1:11319/139‑1 (MQ=255)                                            ccTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGc                                                                                     >  1:499563/1‑139 (MQ=255)                                             cTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCa                                                                                    <  1:409716/139‑1 (MQ=255)                                                   cGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGAtt                                                                              <  1:216155/139‑1 (MQ=255)                                                     tgatgaAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGa                                                                            >  1:82860/1‑139 (MQ=255)                                                     tgatgaAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGa                                                                            <  1:913523/139‑1 (MQ=255)                                                       atgaAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGc                                                                                                             >  2:130297/1‑104 (MQ=255)                                                       atgaAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGc                                                                                                             <  1:130297/104‑1 (MQ=255)                                                            aaTCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGAtt                                                                     >  1:936858/1‑139 (MQ=255)                                                                 caTTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCt                                                                <  2:1158493/139‑1 (MQ=255)                                                                 caTTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCt                                                                <  2:612493/139‑1 (MQ=255)                                                                 caTTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCt                                                                <  2:499563/139‑1 (MQ=255)                                                                       tATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGc                                                          <  2:1026286/139‑1 (MQ=255)                                                                                 gCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGt                                                <  2:524479/139‑1 (MQ=255)                                                                                       tAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACg                                                           <  1:679282/122‑1 (MQ=255)                                                                                       tAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACg                                                           >  2:679282/1‑122 (MQ=255)                                                                                       tAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGt                                          >  2:599020/1‑139 (MQ=255)                                                                                                 ggCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAg                                >  1:1206912/1‑139 (MQ=255)                                                                                                  gcgcCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAgg                               <  2:1206912/139‑1 (MQ=255)                                                                                                               gtgGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAGGCa                             <  1:356963/128‑1 (MQ=255)                                                                                                               gtgGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAGGCa                             >  2:356963/1‑128 (MQ=255)                                                                                                                 ggTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAGGCAACCTTTTATTCAc                <  2:644273/139‑1 (MQ=255)                                                                                                                  gTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCtt                                                               >  2:314929/1‑91 (MQ=255)                                                                                                                  gTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCtt                                                               <  1:314929/91‑1 (MQ=255)                                                                                                                   tctcAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAGGCAACCTTTTATTCACTa              >  1:402065/1‑139 (MQ=255)                                                                                                                    ctcAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCAGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAGGCAACCTTTTATTCACTaa             >  2:607046/1‑139 (MQ=255)                                                                                                                      cAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAGGCAACCTTTTATTCACTAACa           >  1:79095/1‑139 (MQ=255)                                                                                                                        aGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAGGc                              <  2:418162/118‑1 (MQ=255)                                                                                                                        aGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAGGc                              >  1:418162/1‑118 (MQ=255)                                                                                                                              aaGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAGGCAACCTTTTATTCACTAACAAATAGCtg   <  1:607046/139‑1 (MQ=255)                                                                                                                               aGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAGGCAACCTTTTATTCACTAACAAATAGCtgg  <  2:79095/139‑1 (MQ=255)                                                                                                                               aGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAGGCAACCTTTTATTCACTAACAAATAGCtgg  <  1:774071/139‑1 (MQ=255)                                                                                                                               aGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAGGCAACCTTTTATTCACTAACAAATAGCtgg  <  2:936858/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                 GGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGAGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAGGCAACCTTTTATTCACTAACAAATAGCTGG  >  NC_000913/1862498‑1862762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑