Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,926,096 G→A *219* (TAG→TAA)  yobB → C‑N hydrolase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,926,0960GA100.0% 175.4 / NA 55*219* (TAG→TAA) yobBC‑N hydrolase family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/26);  total (29/26)

GGCAAATGCCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTG  >  NC_000913/1925961‑1926230
                                                                                                                                       |                                                                                                                                      
ggCAAATGCCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCt                                                                                                                                     >  2:62942/1‑139 (MQ=255)
   aaaTGCCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCtttt                                                                                                                                  <  2:895306/139‑1 (MQ=255)
   aaaTGCCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCtttt                                                                                                                                  >  2:88233/1‑139 (MQ=255)
     aTGCCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTc                                                                                                                                >  1:1129346/1‑139 (MQ=255)
        cccGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGc                                                                                                                             >  2:293327/1‑139 (MQ=255)
            tGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTgg                                                                                                                         >  1:364230/1‑139 (MQ=255)
                       cACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATg                                                                                                              >  1:1128772/1‑139 (MQ=255)
                        aCTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGt                                                                                                             <  1:238516/139‑1 (MQ=255)
                            tGGGATGAATATGGTCGTCTCATGGTTCGCGCCGATGGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTgcg                                                                                                         <  1:469736/139‑1 (MQ=255)
                            tGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTgcg                                                                                                         >  1:484507/1‑139 (MQ=255)
                                  gAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTAt                                                                                                   <  2:484507/139‑1 (MQ=255)
                                   aaTATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATc                                                                                                  >  1:571795/1‑139 (MQ=255)
                                        ggTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATAc                                                                                             >  1:1141384/1‑139 (MQ=255)
                                           cgtcTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGa                                                                                          <  2:1050203/139‑1 (MQ=255)
                                             tctcATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGcc                                                                                                                 <  1:127182/114‑1 (MQ=255)
                                             tctcATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGcc                                                                                                                 >  2:127182/1‑114 (MQ=255)
                                             tctcATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATc                                                                                                  >  2:400515/1‑129 (MQ=255)
                                             tctcATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATc                                                                                                  <  1:400515/129‑1 (MQ=255)
                                               tcATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAAAAAcc                                                                                      >  1:1012716/1‑139 (MQ=255)
                                                   cGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCg                                                                                  <  2:957966/139‑1 (MQ=255)
                                                        gcgcCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTg                                                                             >  1:839533/1‑139 (MQ=255)
                                                         cgcCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGc                                                                            >  1:948034/1‑139 (MQ=255)
                                                               tCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCagggag                                                                      <  2:839533/139‑1 (MQ=255)
                                                                     ttCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCg                                                                <  2:763851/139‑1 (MQ=255)
                                                                            ttGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGAt                                                         <  1:293327/139‑1 (MQ=255)
                                                                            ttGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGAt                                                         <  2:1141384/139‑1 (MQ=255)
                                                                              gTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACa                                                                                            <  1:253966/102‑1 (MQ=255)
                                                                              gTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACa                                                                                            >  2:253966/1‑102 (MQ=255)
                                                                                 aGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCct                                                    <  2:1129346/139‑1 (MQ=255)
                                                                                    cGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTg                                                 >  1:816252/1‑139 (MQ=255)
                                                                                      gTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTAt                                                                                                   <  1:934796/87‑1 (MQ=255)
                                                                                      gTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTAt                                                                                                   >  2:934796/1‑87 (MQ=255)
                                                                                      gTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCg                                                                                                 <  1:817787/89‑1 (MQ=255)
                                                                                      gTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCg                                                                                                 >  2:817787/1‑89 (MQ=255)
                                                                                      gTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGtt                                               >  1:466730/1‑139 (MQ=255)
                                                                                       tCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTAt                                                                                                   >  2:205444/1‑86 (MQ=255)
                                                                                       tCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTAt                                                                                                   <  1:205444/86‑1 (MQ=255)
                                                                                                     aGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCg                                                                                                 <  1:493993/74‑1 (MQ=255)
                                                                                                     aGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCg                                                                                                 >  2:493993/1‑74 (MQ=255)
                                                                                                      gggTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTAt                                                                                                   >  1:385822/1‑71 (MQ=255)
                                                                                                      gggTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTAt                                                                                                   <  2:385822/71‑1 (MQ=255)
                                                                                                           ggCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCct                                                    >  1:655255/1‑113 (MQ=255)
                                                                                                           ggCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCct                                                    <  2:655255/113‑1 (MQ=255)
                                                                                                               aGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCa                      <  2:816252/139‑1 (MQ=255)
                                                                                                                 gCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAAc                    >  1:43441/1‑139 (MQ=255)
                                                                                                                 gCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAAc                    >  1:942077/1‑139 (MQ=255)
                                                                                                                    atatCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAAcccc                 <  2:364230/139‑1 (MQ=255)
                                                                                                                     tatCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCt                                                                                     >  1:1124962/1‑70 (MQ=255)
                                                                                                                     tatCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCt                                                                                     <  2:1124962/70‑1 (MQ=255)
                                                                                                                           tCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCAt                                                                                                      >  2:1149771/1‑47 (MQ=255)
                                                                                                                           tCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCAt                                                                                                      <  1:1149771/47‑1 (MQ=255)
                                                                                                                           tCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGAt                                                                                               <  2:794557/54‑1 (MQ=255)
                                                                                                                           tCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGAt                                                                                               >  1:794557/1‑54 (MQ=255)
                                                                                                                                 aCGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTgg    <  2:466730/139‑1 (MQ=255)
                                                                                                                                   gCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTg  >  1:947498/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                      
GGCAAATGCCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTG  >  NC_000913/1925961‑1926230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑