Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,977,529 A→G C106R (TGC→CGC)  flhC ← flagellar class II regulon transcriptional activator, with FlhD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,977,5290AG100.0% 143.3 / NA 41C106R (TGC→CGC) flhCflagellar class II regulon transcriptional activator, with FlhD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/21);  total (20/21)

TGGGTAATAAAATTGCCGCCGCAGCAGTTGCAGCTGGAAAGTTGCAGTAATCCACTTTCAACAAACCGCACCAATGTCCAGGCACGGGTTAATGCCAGCAGTGGTCCTTCTTCTGCTTGTGGGCACTGTTCAAGGTATAAACGGTAGGCTTTGATCACCGCATCGACGCCATTACACAAACCGGTTTTCAGTAAAAACTGCCATGCATTACAGAACATCGAAGCATGAACGTTTTGTTCCCAGGTCATAAACC  >  NC_000913/1977405‑1977657
                                                                                                                            |                                                                                                                                
tGGGTAATAAAATTGCCGCCGCAGCAGTTGCAGCTGGAAAGTTGCAGTAATCCACTTTCAACAAACCGCACCAATGTCCAGGCACGGGTTAATGCCAGCAGTGGTCCTTCTTCTGCTTGTGGGCGCTGtt                                                                                                                             <  2:246103/130‑1 (MQ=255)
tGGGTAATAAAATTGCCGCCGCAGCAGTTGCAGCTGGAAAGTTGCAGTAATCCACTTTCAACAAACCGCACCAATGTCCAGGCACGGGTTAATGCCAGCAGTGGTCCTTCTTCTGCTTGTGGGCGCTGtt                                                                                                                             >  1:246103/1‑130 (MQ=255)
    taataaAATTGCCGCCGCAGCAGTTGCAGCTGGAAAGTTGCAGTAATCCACTTTCAACAAACCGCACCAATGTCCAGGCACGGGTTAATGCCAGCAGTGGTCCTTCTTCTGCTTGTGGGCGCTGTTCaa                                                                                                                          <  2:608720/129‑1 (MQ=255)
    taataaAATTGCCGCCGCAGCAGTTGCAGCTGGAAAGTTGCAGTAATCCACTTTCAACAAACCGCACCAATGTCCAGGCACGGGTTAATGCCAGCAGTGGTCCTTCTTCTGCTTGTGGGCGCTGTTCaa                                                                                                                          >  1:608720/1‑129 (MQ=255)
      ataaAATTGCCGCCGCAGCAGTTGCAGCTGGAAAGTTGCAGTAATCCACTTTCAACAAACCGCACCAATGTCCAGGCACGGGTTAATGCCAGCAGTGGTCCTTCTTCTGCTTGTGGGCGCTGTTCAAGGTATAAACGGt                                                                                                              <  2:1101662/139‑1 (MQ=255)
           aTTGCCGCCGCAGCAGTTGCAGCTGGAAAGTTGCAGTAATCCACTTTCAACAAACCGCACCAATGTCCAGGCACGGGTTAATGCCAGCAGTGGTCCTTCTTCTGCTTGTGGGCGCTGTTCAAGGTATAAACGGTAGGCt                                                                                                         >  2:1132531/1‑139 (MQ=255)
             tGCCGCCGCAGCAGTTGCAGCTGGAAAGTTGCAGTAATCCACTTTCAACAAACCGCACCAATGTCCAGGCACGGGTTAATGCCAGCAGTGGTCCTTCTTCTGCTTGTGGGCGCTGTTCAAGGTATAAACGGTAGGCttt                                                                                                       >  2:960879/1‑139 (MQ=255)
                                gCTGGAAAGTTGCAGTAATCCACTTTCAACAAACCGCACCAATGTCCAGGCCCGGGTTAATGCCAGCAGTGGTCCTTCTTCTGCTTGTGGGCGCTGTTCAAGGTATAAACGGTAGGCTTTGATGACCGCATCGACGCCa                                                                                    <  1:813464/139‑1 (MQ=255)
                                                         tCAACAAACCGCACCAATGTCCAGGCACGGGTTAATGCCAGCAGTGGTCCTTCTTCTGCTTGTGGGCGCTGTTCAAGGTATAAACGGt                                                                                                              >  2:433596/1‑88 (MQ=255)
                                                         tCAACAAACCGCACCAATGTCCAGGCACGGGTTAATGCCAGCAGTGGTCCTTCTTCTGCTTGTGGGCGCTGTTCAAGGTATAAACGGt                                                                                                              <  1:433596/88‑1 (MQ=255)
                                                                aCCGCACCAATGTCCAGGAACGGGTTAATGCCAGCAGTGGTCCTTCTTCTGCTTGTGGGCGCTGTTCAAGGTATAAACGGTAGGCTTTCATCGCCGCATCGACGCCATTACACAATCCGGTTTTCAGTAAAAACTGCCa                                                    <  1:977232/139‑1 (MQ=255)
                                                                 ccGCACCAATGTCCAGGCACGGGTTAATGCCAGCAGTGGTCCTTCTTCTGCTTGTGGGCGCTGTTCAAg                                                                                                                         <  1:851406/69‑1 (MQ=255)
                                                                 ccGCACCAATGTCCAGGCACGGGTTAATGCCAGCAGTGGTCCTTCTTCTGCTTGTGGGCGCTGTTCAAg                                                                                                                         >  2:851406/1‑69 (MQ=255)
                                                                       cAATGTCCAGGCACGGGTTAATGCCAGCAGTGGTCCTTCTTCTGCTTGTGGGCGCTGTTCAAGGTATAAACGGTAGGCTTTGATCACCGCATCGACGCCATTACACAAACCGGTTTTCAGTAAAAACTGCCATGCATTa                                             <  1:1132531/139‑1 (MQ=255)
                                                                                 gCACGGGTTAATGCCAGCAGTGGTCCTTCTTCTGCTTGTGGGCGCTGtt                                                                                                                             >  2:807101/1‑49 (MQ=255)
                                                                                 gCACGGGTTAATGCCAGCAGTGGTCCTTCTTCTGCTTGTGGGCGCTGtt                                                                                                                             <  1:807101/49‑1 (MQ=255)
                                                                                      ggTTAATGCCAGCAGTGGTCCTTCTTCTGCTTGTGGGCGCTGTTCaa                                                                                                                          <  2:832863/47‑1 (MQ=255)
                                                                                      ggTTAATGCCAGCAGTGGTCCTTCTTCTGCTTGTGGGCGCTGTTCaa                                                                                                                          >  1:832863/1‑47 (MQ=255)
                                                                                      ggTTAATGCCAGCAGTGGTCCTTCTTCTGCTTGTGGGCGCTGTTCAAGGTATAAACGGTAGGCTTTGATCACCGCATCGACGCCATTACACAAACCGGTTTTCAGTAAAAACTGCCATGCATTACAGAACATCGAAGCa                              >  2:776984/1‑139 (MQ=255)
                                                                                           aTGCCAGCAGTGGTCCTTCTTCTGCTTGTGGGCGCTGTTCAAGGTATAAACGGTAGGCTTTGATCACCGCATCGACGCCATTACACAAACCGGTTTTCAGTAAAAACTGCCATg                                                  >  1:476255/1‑114 (MQ=255)
                                                                                           aTGCCAGCAGTGGTCCTTCTTCTGCTTGTGGGCGCTGTTCAAGGTATAAACGGTAGGCTTTGATCACCGCATCGACGCCATTACACAAACCGGTTTTCAGTAAAAACTGCCATg                                                  <  2:476255/114‑1 (MQ=255)
                                                                                                 gcagTGGTCCTTCTTCTGCTTGTGGGCGCTGTTCAAGGTATAAACGGTAGGCTTTGATCACCGCATCGACGCCATTACACAAACCGGTTTTCAGTAAAAACTGCCATGCATTACAGAACATCGAAGCATGAACGTTTTg                   >  2:508709/1‑139 (MQ=255)
                                                                                                      ggTCCTTCTTCTGCTTGTGGGCGCTGTTCAAGGTATAAACGGTAGGCTTTGATCACCGCATCGACGCCATTACACAAACCGGTTTTCAGTAAAAACTGCCATGCATTa                                             >  1:1194234/1‑108 (MQ=255)
                                                                                                      ggTCCTTCTTCTGCTTGTGGGCGCTGTTCAAGGTATAAACGGTAGGCTTTGATCACCGCATCGACGCCATTACACAAACCGGTTTTCAGTAAAAACTGCCATGCATTa                                             <  2:1194234/108‑1 (MQ=255)
                                                                                                      ggTCCTTCTTCTGCTTGTGGGCGCTGTTCAAGGTATAAACGGTAGGCTTTGATCACCGCATCGACGCCATTACACAAACCGGTTTTCAGTAAAAACTGCCATGCATTACAGAACATCGAAGCATGAACGTTTTGTTccc              <  1:776984/139‑1 (MQ=255)
                                                                                                      ggTCCTTCTTCTGCTTGTGGGCGCTGTTCAAGGTATAAACGGTAGGCTTTGATCACCGCATCGACGCCATTACACAAACCGGTTTTCAGTAAAAACTGCCATGCATTACAGAACATCGAAGCATGAACGTTTTGTTccc              <  2:322870/139‑1 (MQ=255)
                                                                                                      ggTCCTTCTTCTGCTTGTGGGCGCTGTGCAAGCTATAAACGGTAGGCTTTGATCACCGCATCGACGCCATTACACAAACCGGTTTTCAGTAAAAACTGCCATGCATTa                                             >  1:725436/1‑108 (MQ=255)
                                                                                                      ggTCCTTCTTCTGCTTATGGGCGCTGTTCAAGGTATAAACGGTAGGCTTTGATCACCGCATCGACGCCATTACACAAACCGGTTTTCAGTAAAAACTGCCATGCATTa                                             <  2:725436/108‑1 (MQ=255)
                                                                                                       gTCCTTCTTCTGCTTGTGGGCGCTGTTCAAGGTATAAACGGTAGGCTTTGATCACCGCATCGACGCCATTACACAAGCCGGTTTTCAGTAAAAACTGcc                                                     >  1:872331/1‑99 (MQ=255)
                                                                                                       gTCCTTCTTCTGCTTGTGGGCGCTGTTCAAGGTATAAACGGTAGGCTTTGATCACCGCATCGACGCCATTACACAAACCGGTTTTCAGTAAAAACTGcc                                                     <  2:872331/99‑1 (MQ=255)
                                                                                                        tCCTTCTTCTGCTTGTGGGCGCTGTTCAAGGTATAAACGGTAGGCTTTGATCACCGCATCGACGCCATTACACAAACCGGTTTTCAGTAAAAACTGCCATg                                                  >  1:708217/1‑101 (MQ=255)
                                                                                                        tCCTTCTTCTGCTTGTGGGCGCTGTTCAAGGTATAAACGGTAGGCTTTGATCACCGCATCGACGCCATTACACAAACCGGTTTTCAGTAAAAACTGCCATg                                                  <  2:708217/101‑1 (MQ=255)
                                                                                                              ttctGCTTGTGGGCGCTGTTCAAGGTATAAACGGTAGGCTTTGATCACCGCATCGa                                                                                         >  2:425181/1‑56 (MQ=255)
                                                                                                              ttctGCTTGTGGGCGCTGTTCAAGGTATAAACGGTAGGCTTTGATCACCGCATCGa                                                                                         <  1:425181/56‑1 (MQ=255)
                                                                                                                 tGCTTGTGGGCGCTGTTCAAGGTATAAACGGTAGGCTTTGATCACCGCATCGACGCCATTACACAAACCGGTTTTCAGTAAAAACTGCCATGCa                                                >  1:629041/1‑94 (MQ=255)
                                                                                                                 tGCTTGTGGGCGCTGTTCAAGGTATAAACGGTAGGCTTTGATCACCGCATCGACGCCATTACACAAACCGGTTTTCAGTAAAAACTGCCATGCa                                                <  2:629041/94‑1 (MQ=255)
                                                                                                                  gCTTGTAGGCGCTGTTCAAGGTATAAACGGTAGGCTTTGATCACCGCATCGACGCCATTACACAAACCGGTTTTCAGTAAAAACTGCCATGCATTACAGAACATCGAAGCATGAACGTTTTGTTCCCAGGTCATAAAcc  >  1:10347/1‑139 (MQ=255)
                                                                                                                      gtgGGCGCTGTTCAAGGTATAAACGGTAGGCTTTGATCACCGCATCGACGCCATTACACAAACCGGTTTTCAGTAAAAACTGCCATg                                                  >  2:207311/1‑87 (MQ=255)
                                                                                                                      gtgGGCGCTGTTCAAGGTATAAACGGTAGGCTTTGATCACCGCATCGACGCCATTACACAAACCGGTTTTCAGTAAAAACTGCCATg                                                  <  1:207311/87‑1 (MQ=255)
                                                                                                                       tgGGCGCTGTTCAAGGTATAAACGGTAGGCTTTGATCACCGCATCGa                                                                                         <  1:1169434/47‑1 (MQ=255)
                                                                                                                       tgGGCGCTGTTCAAGGTATAAACGGTAGGCTTTGATCACCGCATCGa                                                                                         >  2:1169434/1‑47 (MQ=255)
                                                                                                                            |                                                                                                                                
TGGGTAATAAAATTGCCGCCGCAGCAGTTGCAGCTGGAAAGTTGCAGTAATCCACTTTCAACAAACCGCACCAATGTCCAGGCACGGGTTAATGCCAGCAGTGGTCCTTCTTCTGCTTGTGGGCACTGTTCAAGGTATAAACGGTAGGCTTTGATCACCGCATCGACGCCATTACACAAACCGGTTTTCAGTAAAAACTGCCATGCATTACAGAACATCGAAGCATGAACGTTTTGTTCCCAGGTCATAAACC  >  NC_000913/1977405‑1977657

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑