Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,049,336	C→T	P1467S (CCG→TCG)	yeeJ →	putative adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,049,336	0	C	T	100.0%	161.3 / NA	50	P1467S (CCG→TCG)	yeeJ	putative adhesin
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/30);  total (20/30)

TTACCGCTACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATCCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTACTCTGCCGGAAGATGTGAAGGCGAACTTCACGCTGAGCGA  >  NC_000913/2049208‑2049469                                                                                                                                 |                                                                                                                                      ttACCGCTACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGc                                                                                                                             <  2:5232/139‑1 (MQ=255)  tACCGCTACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGcc                                                                                                                            <  1:1206161/139‑1 (MQ=255)  tACCGCTACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGcc                                                                                                                            <  2:731476/139‑1 (MQ=255)    ccGCTACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGa                                                                                                                          <  2:31072/139‑1 (MQ=255)         aCAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCCCGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATa                                                                                                                     >  2:506348/1‑139 (MQ=255)          cAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATaa                                                                                                                    >  2:976865/1‑139 (MQ=255)          cAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAAAAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGACTCGAAGGATTCGGTGATTGCCGACAATaa                                                                                                                    >  2:127988/1‑139 (MQ=255)             aaGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGa                                                                                                                 <  1:443372/139‑1 (MQ=255)                 cGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGAtctc                                                                                                             <  1:976865/139‑1 (MQ=255)                     gTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGa                                                                                                         >  1:1146102/1‑139 (MQ=255)                       ttATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGaca                                                                                                       <  1:433263/139‑1 (MQ=255)                         aTCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACt                                                                                                     <  2:999540/139‑1 (MQ=255)                                gACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGa                                                                                                                          >  1:257773/1‑111 (MQ=255)                                gACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGa                                                                                                                          <  2:257773/111‑1 (MQ=255)                                   ggCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACa                                                                                           <  2:13702/139‑1 (MQ=255)                                       aCCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCg                                                                                       <  1:1202991/139‑1 (MQ=255)                                        cccTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGt                                                                                         <  1:1094204/136‑1 (MQ=255)                                        cccTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGt                                                                                         >  2:1094204/1‑136 (MQ=255)                                         ccTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCt                                                                                     <  1:765616/139‑1 (MQ=255)                                           tCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGa                                                                                   >  2:561976/1‑139 (MQ=255)                                                 aTGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACaga                                                                             <  1:944697/139‑1 (MQ=255)                                                   ggCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAgg                                                                           >  1:804037/1‑139 (MQ=255)                                                       aTTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTg                                                                                    <  2:999022/126‑1 (MQ=255)                                                       aTTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTg                                                                                    >  1:999022/1‑126 (MQ=255)                                                       aTTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCaa                                                                       >  2:884604/1‑139 (MQ=255)                                                           gATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCg                                                                   <  2:804037/139‑1 (MQ=255)                                                             tGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGAt                                                                 <  1:673533/139‑1 (MQ=255)                                                               caacaaACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAg                                                               <  1:127988/139‑1 (MQ=255)                                                                     acaGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAaca                                                         <  1:848672/139‑1 (MQ=255)                                                                      caGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAaca                                                         <  1:656890/138‑1 (MQ=255)                                                                      caGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAaca                                                         >  2:656890/1‑138 (MQ=255)                                                                          gACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAg                                                    >  2:534472/1‑139 (MQ=255)                                                                            ccTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACa                                                                                           >  1:121770/1‑98 (MQ=255)                                                                            ccTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACa                                                                                           <  2:121770/98‑1 (MQ=255)                                                                            ccTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGt                                                  >  2:186365/1‑139 (MQ=255)                                                                                       aaCGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGcc                                                             <  2:619028/117‑1 (MQ=255)                                                                                       aaCGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGcc                                                             >  1:619028/1‑117 (MQ=255)                                                                                       aaCGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAaca                                                         <  1:997941/121‑1 (MQ=255)                                                                                       aaCGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAaca                                                         >  2:997941/1‑121 (MQ=255)                                                                                              cgAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTACTCTGCCGGa                                <  1:506348/139‑1 (MQ=255)                                                                                              cgAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTACTCTGCCGGa                                <  1:561976/139‑1 (MQ=255)                                                                                               gAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTACTCTGCCGGaa                               >  2:43665/1‑139 (MQ=255)                                                                                                     tGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTACTCTGCCGGAAGAtgtg                         <  2:1146102/139‑1 (MQ=255)                                                                                                          tCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACa                                                                               <  2:945513/80‑1 (MQ=255)                                                                                                          tCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACa                                                                               >  1:945513/1‑80 (MQ=255)                                                                                                          tCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTACTCTGCCGGAAGATGTGAAGGc                    <  1:549584/139‑1 (MQ=255)                                                                                                              gCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACa                                                                               <  2:496974/76‑1 (MQ=255)                                                                                                              gCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACa                                                                               >  1:496974/1‑76 (MQ=255)                                                                                                              gCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAAAATTTACTCTGCCGGAAGATGTGAAGGCGAAc                >  2:579071/1‑139 (MQ=255)                                                                                                                            aGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTACTCTGCCGGAAGATGTGAAGGCGAACTTCACGCTGAGCGa  <  2:669054/139‑1 (MQ=255)                                                                                                                                 |                                                                                                                                      TTACCGCTACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATCCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTACTCTGCCGGAAGATGTGAAGGCGAACTTCACGCTGAGCGA  >  NC_000913/2049208‑2049469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑