Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,059,964 C→T *317* (TAG→TAA)  cbl ← ssuEADCB/tauABCD operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,059,9640CT98.0% 157.0 / NA 50*317* (TAG→TAA) cblssuEADCB/tauABCD operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (26/23);  minor base A (1/0);  total (27/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCTCGCTTC  >  NC_000913/2059838‑2060093
                                                                                                                              |                                                                                                                                 
ccTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGTGGAGCCAAGTGCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCGGATaa                                                                                                                       >  1:326574/1‑139 (MQ=21)
ccTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATaa                                                                                                                       >  1:183237/1‑139 (MQ=255)
    aacaacGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCaa                                                                                                                   >  1:1208305/1‑139 (MQ=255)
                tctGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCAc                                                                                                       <  1:405364/139‑1 (MQ=255)
                       ttCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTc                                                                                                >  1:214885/1‑139 (MQ=255)
                         cAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCa                                                                                              >  1:945690/1‑139 (MQ=255)
                         cAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCa                                                                                              >  1:1029615/1‑139 (MQ=255)
                                   aaCGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCg                                                                                    <  2:652570/139‑1 (MQ=255)
                                       gcggACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTg                                                                                <  2:214885/139‑1 (MQ=255)
                                          gACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTc                                                                                                <  2:1170636/120‑1 (MQ=255)
                                          gACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTc                                                                                                >  1:1170636/1‑120 (MQ=255)
                                          gACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGata                                                                             >  1:475198/1‑139 (MQ=255)
                                          gACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGata                                                                             >  1:989759/1‑139 (MQ=255)
                                                     cGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGa                                                                  <  2:475198/139‑1 (MQ=255)
                                                     cGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGa                                                                  <  2:183237/139‑1 (MQ=255)
                                                     cGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGa                                                                  >  1:564937/1‑139 (MQ=255)
                                                       tATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAAt                                                                                                                      >  2:977951/1‑85 (MQ=255)
                                                       tATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAAt                                                                                                                      <  1:977951/85‑1 (MQ=255)
                                                         tGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTAGATATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGt                                                              >  1:710622/1‑139 (MQ=255)
                                                          gTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTc                                                             <  2:68688/139‑1 (MQ=255)
                                                             aCTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTg                                                          >  1:223197/1‑139 (MQ=255)
                                                              cTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGa                                                                  >  1:495555/1‑130 (MQ=255)
                                                              cTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGa                                                                  <  2:495555/130‑1 (MQ=255)
                                                               tGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCt                                                           <  1:965719/136‑1 (MQ=255)
                                                               tGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCt                                                           >  2:965719/1‑136 (MQ=255)
                                                                 gTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTc                                                                                                               <  2:172476/82‑1 (MQ=255)
                                                                 gTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTc                                                                                                               >  1:172476/1‑82 (MQ=255)
                                                                 gTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTg                                                                                >  2:788540/1‑113 (MQ=255)
                                                                 gTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTg                                                                                <  1:788540/113‑1 (MQ=255)
                                                                     gAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCATCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACaa                                                  >  1:942357/1‑139 (MQ=255)
                                                                      aGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGc                                                                                      <  2:900245/102‑1 (MQ=255)
                                                                      aGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGc                                                                                      >  1:900245/1‑102 (MQ=255)
                                                                      aGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACaaa                                                 >  2:992719/1‑139 (MQ=255)
                                                                                 aggagCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTg                                                                                                     <  1:878702/76‑1 (MQ=255)
                                                                                 aggagCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTg                                                                                                     >  2:878702/1‑76 (MQ=255)
                                                                                 aggagCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAg                                      >  2:38763/1‑139 (MQ=255)
                                                                                     gCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGcc                                  <  1:38763/139‑1 (MQ=255)
                                                                                      ccAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCa                                 >  2:969124/1‑139 (MQ=255)
                                                                                             ccTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAg                          <  2:223197/139‑1 (MQ=255)
                                                                                              cTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGt                         <  2:989759/139‑1 (MQ=255)
                                                                                                      cccGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAgcg                                    >  1:177383/1‑120 (MQ=255)
                                                                                                      cccGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAgcg                                    <  2:177383/120‑1 (MQ=255)
                                                                                                        cGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAg               <  2:1075531/139‑1 (MQ=255)
                                                                                                               ataGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCt        <  1:969124/139‑1 (MQ=255)
                                                                                                                    gggATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAgcgc                                   >  2:393241/1‑107 (MQ=255)
                                                                                                                    gggATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAgcgc                                   <  1:393241/107‑1 (MQ=255)
                                                                                                                     ggATTTTTGTTATATCTGGTAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGaaa                                       >  1:677761/1‑102 (MQ=255)
                                                                                                                     ggATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGaaa                                       <  2:677761/102‑1 (MQ=255)
                                                                                                                     ggATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCTCGCTTc  >  2:328781/1‑139 (MQ=255)
                                                                                                                       aTTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCa                                 >  2:1190602/1‑106 (MQ=255)
                                                                                                                       aTTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCa                                 <  1:1190602/106‑1 (MQ=255)
                                                                                                                              |                                                                                                                                 
CCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCTCGCTTC  >  NC_000913/2059838‑2060093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑