Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,068,952	C→T	*302* (TAG→TAA)	insD1 ←	IS2 transposase TnpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,068,952	0	C	T	100.0%	203.6 / NA	63	*302* (TAG→TAA)	insD1	IS2 transposase TnpB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/31);  total (32/31)

TTATTGACCAACAAACATTGCAGAACTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCT  >  NC_000913/2068816‑2069075                                                                                                                                         |                                                                                                                            ttATTGACCAACAAACATTGCAGAACTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTta                                                                                                                           <  2:359515/139‑1 (MQ=255)   aTTGACCAACAAACATTGCAGAACTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTtata                                                                                                                         >  1:580028/1‑139 (MQ=255)     tGACCAACAAACATTGCAGAACTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATAtt                                                                                                                       >  2:1130045/1‑139 (MQ=255)     tGACCAACAAACATTGCAGAACTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCACATTCGTGGCTGGATTTGCCCTTATAtt                                                                                                                       >  1:1191689/1‑139 (MQ=255)     tGACCAACAAACATTGCAGAACTACACCAATGCCTCCATCGCCGCTAATTTGCAGGACATCCCCGGAGTAGAGATAACAACCAACTCCTTGGCAGGCCGTAAACACATCCCCACTCGTGGCTTGATTTACCCTTATAtt                                                                                                                       >  1:975537/1‑139 (MQ=255)         caacaaACATTGCAGAACTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCa                                                                                                                   <  2:1118592/139‑1 (MQ=255)            caaacaTTGCAGAACTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGAc                                                                                                                >  2:753047/1‑139 (MQ=255)                   tGCAGAACTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTta                                                                                                                           >  1:900488/1‑121 (MQ=255)                   tGCAGAACTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTta                                                                                                                           <  2:900488/121‑1 (MQ=255)                         aCTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACACCTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACt                                                                                                   <  1:962604/139‑1 (MQ=255)                          cTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACtt                                                                                                  >  1:935445/1‑139 (MQ=255)                          cTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACtt                                                                                                  >  1:1157054/1‑139 (MQ=255)                           tCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACAt                                                                                                              <  2:506397/126‑1 (MQ=255)                           tCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACAt                                                                                                              >  1:506397/1‑126 (MQ=255)                                          aTAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGccc                                                                                  >  2:676529/1‑139 (MQ=255)                                           tAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCg                                                                                 <  2:835252/139‑1 (MQ=255)                                                gATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGcc                                                                            <  2:580028/139‑1 (MQ=255)                                                gATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGcc                                                                            <  2:986217/139‑1 (MQ=255)                                                        gCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCg                                                                           >  1:945188/1‑132 (MQ=255)                                                        gCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCg                                                                           <  2:945188/132‑1 (MQ=255)                                                         cAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGcccc                                                                                                                             <  1:852142/81‑1 (MQ=255)                                                         cAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGcccc                                                                                                                             >  2:852142/1‑81 (MQ=255)                                                         cAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATAtt                                                                   <  2:1157054/139‑1 (MQ=255)                                                            gACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGc                                                                          >  1:864117/1‑129 (MQ=255)                                                            gACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGc                                                                          <  2:864117/129‑1 (MQ=255)                                                             aCATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCa                                                                                                                   <  2:881740/87‑1 (MQ=255)                                                             aCATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCa                                                                                                                   >  1:881740/1‑87 (MQ=255)                                                                 ccccGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGgc                                                           >  2:957123/1‑139 (MQ=255)                                                                  cccGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGgcg                                                          <  2:69995/139‑1 (MQ=255)                                                                  cccGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGgcg                                                          <  1:223979/139‑1 (MQ=255)                                                                      gAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGgcgagcg                                                      >  2:695982/1‑139 (MQ=255)                                                                        gTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGAt                                                    <  2:932377/139‑1 (MQ=255)                                                                           gagaTAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAAc                                                 >  2:265701/1‑139 (MQ=255)                                                                            agaTAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACt                                                                                                   >  2:354643/1‑88 (MQ=255)                                                                            agaTAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACt                                                                                                   <  1:354643/88‑1 (MQ=255)                                                                               tAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAg                                             <  2:935445/139‑1 (MQ=255)                                                                                aaCAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCg                                                                                 <  2:927068/102‑1 (MQ=255)                                                                                aaCAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCg                                                                                 >  1:927068/1‑102 (MQ=255)                                                                                aaCAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATa                                                   <  1:237005/132‑1 (MQ=255)                                                                                aaCAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATa                                                   >  2:237005/1‑132 (MQ=255)                                                                                 aCAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGgcg                                                          <  2:133430/124‑1 (MQ=255)                                                                                 aCAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGgcg                                                          >  1:133430/1‑124 (MQ=255)                                                                                 aCAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATa                                                   <  2:286575/131‑1 (MQ=255)                                                                                 aCAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATa                                                   >  1:286575/1‑131 (MQ=255)                                                                                   aGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCa                                         <  1:753047/139‑1 (MQ=255)                                                                                   aGACAACTCCTTGGCAGGCCGTAAACAAATCCACATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCa                                         <  2:1191689/139‑1 (MQ=255)                                                                                      cAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTa                                      >  2:560711/1‑139 (MQ=255)                                                                                       aaCTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACAt                                                                                                              <  2:910746/66‑1 (MQ=255)                                                                                       aaCTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACAt                                                                                                              >  1:910746/1‑66 (MQ=255)                                                                                        aCTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATg                                    >  1:1150852/1‑139 (MQ=255)                                                                                         cTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGc                                   >  1:621714/1‑139 (MQ=255)                                                                                           ccTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCgg                                 >  2:368922/1‑139 (MQ=255)                                                                                               ggCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGc                             >  1:939445/1‑139 (MQ=255)                                                                                                 cAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTa                                                                                                        <  1:373806/62‑1 (MQ=255)                                                                                                 cAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTa                                                                                                        >  2:373806/1‑62 (MQ=255)                                                                                                  aGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCAt                          <  2:621714/139‑1 (MQ=255)                                                                                                  aGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCAt                          <  1:957123/139‑1 (MQ=255)                                                                                                    gCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCa                                                                                                                   >  1:113445/1‑48 (MQ=255)                                                                                                    gCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCa                                                                                                                   <  2:113445/48‑1 (MQ=255)                                                                                                    gCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTc                        >  2:178810/1‑139 (MQ=255)                                                                                                    gCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTc                        >  2:171302/1‑139 (MQ=255)                                                                                                               aTCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGcc                            >  2:501629/1‑124 (MQ=255)                                                                                                               aTCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGcc                            <  1:501629/124‑1 (MQ=255)                                                                                                                  cGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGa          >  1:450620/1‑139 (MQ=255)                                                                                                                   gCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGaa         <  1:178810/139‑1 (MQ=255)                                                                                                                          tGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCctct  <  1:695982/139‑1 (MQ=17)                                                                                                                                         |                                                                                                                            TTATTGACCAACAAACATTGCAGAACTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCT  >  NC_000913/2068816‑2069075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑