Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,226,509	C→T	*254* (TAG→TAA)	yohF ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,226,509	0	C	T	100.0%	118.0 / NA	37	*254* (TAG→TAA)	yohF	putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/17);  total (20/17)

CGTAAAACCATTGCCCTGGATAAGGCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTC‑TTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCATGCGTT  >  NC_000913/2226375‑2226632                                                                                                                                        |                                                                                                                            cGTAAAACCATTGCCCTGGATAAGGCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTC‑TTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTAtt                                                                                                                         <  1:90295/139‑1 (MQ=255)                   gATAAGGCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTC‑TTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTAtt                                                                                                                         <  2:255467/121‑1 (MQ=255)                   gATAAGGCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTC‑TTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTAtt                                                                                                                         >  1:255467/1‑121 (MQ=255)                        ggCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTC‑TTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGcc                                                                                                  >  2:1108888/1‑139 (MQ=255)                                           aCATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTC‑TTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCa                                                                               <  2:628921/139‑1 (MQ=255)                                                         aTGCCTGATACGACGCTTGTGCGTC‑TTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCa                                                                               >  1:1150155/1‑125 (MQ=255)                                                         aTGCCTGATACGACGCTTGTGCGTC‑TTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCa                                                                               <  2:1150155/125‑1 (MQ=255)                                                         aTGCCTGATACGACGCTTGTGCGTC‑TTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGc                                                                 >  1:615005/1‑139 (MQ=255)                                                          tGCCTGATACGACGCTTGTGCGTCTTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCa                                                                               <  1:534421/125‑1 (MQ=255)                                                          tGCCTGATACGACGCTTGTGCGTCTTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCa                                                                               >  2:534421/1‑125 (MQ=255)                                                          tGCCTGATACGACGCTTGTGCGTC‑TTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGcc                                                                <  1:913879/139‑1 (MQ=255)                                                          tGCCTGATACGACGCTTGTGCGTC‑TTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGcc                                                                >  2:494756/1‑139 (MQ=255)                                                          tGCCTGATACGACGCTTGTGCGTC‑TTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGcc                                                                <  2:615005/139‑1 (MQ=255)                                                          tGCCTGATACGACGCTTGTGCGTC‑TTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGcc                                                                >  2:765869/1‑139 (MQ=255)                                                                      cgCTTGTGCGTC‑TTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAAt                                                    >  2:685738/1‑139 (MQ=255)                                                                      cgCTTGTGCGTC‑TTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAAt                                                    >  2:706201/1‑139 (MQ=255)                                                                           gtgCGTC‑TTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGa                                                                     <  2:1154153/117‑1 (MQ=255)                                                                           gtgCGTC‑TTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGa                                                                     >  1:1154153/1‑117 (MQ=255)                                                                            tgCGTC‑TTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCg                                                              >  1:391664/1‑123 (MQ=255)                                                                            tgCGTC‑TTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCg                                                              <  2:391664/123‑1 (MQ=255)                                                                                    tttCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCa                                                                               >  2:678275/1‑99 (MQ=255)                                                                                    tttCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCa                                                                               <  1:678275/99‑1 (MQ=255)                                                                                         tGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACaaa                                  <  1:1108888/139‑1 (MQ=255)                                                                                             gACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACaa                                   <  2:956000/134‑1 (MQ=255)                                                                                             gACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACaa                                   >  1:956000/1‑134 (MQ=255)                                                                                                             aaTGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAgccg                                                                                      <  2:446084/67‑1 (MQ=255)                                                                                                             aaTGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAgccg                                                                                      >  1:446084/1‑67 (MQ=255)                                                                                                             aaTGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCa              >  2:447903/1‑139 (MQ=255)                                                                                                                         aCGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCATGCGtt  >  1:774232/1‑139 (MQ=255)                                                                                                                            cACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGc                   <  1:780094/119‑1 (MQ=255)                                                                                                                            cACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGc                   >  2:780094/1‑119 (MQ=255)                                                                                                                              ccTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCgg                                                             >  2:453608/1‑75 (MQ=255)                                                                                                                              ccTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCgg                                                             <  1:453608/75‑1 (MQ=255)                                                                                                                                tCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTaa                                                     >  2:478700/1‑81 (MQ=255)                                                                                                                                tCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTaa                                                     <  1:478700/81‑1 (MQ=255)                                                                                                                                 cACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCgg                                                             >  2:144250/1‑72 (MQ=255)                                                                                                                                 cACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCgg                                                             <  1:144250/72‑1 (MQ=255)                                                                                                                                        |                                                                                                                            CGTAAAACCATTGCCCTGGATAAGGCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTC‑TTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCATGCGTT  >  NC_000913/2226375‑2226632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑