| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,597,904 | G→A | P321L (CCG→CTG) | bamC ← | BamABCDE complex OM biogenesis lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,597,904 | 0 | G | A | 97.8% | 140.9 / NA | 46 | P321L (CCG→CTG) | bamC | BamABCDE complex OM biogenesis lipoprotein |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (20/25); total (21/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGACGCACACGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACG > NC_000913/2597769‑2598035 | cAGACGCACACGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGAt < 2:478844/139‑1 (MQ=255) aCGCACACGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGa > 2:903220/1‑139 (MQ=255) cGCACACGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGAt < 2:211958/139‑1 (MQ=255) gCACACGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGATg < 1:6256/139‑1 (MQ=255) gCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGgc < 1:310313/139‑1 (MQ=255) atcatatcaGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGgctgct > 1:985640/1‑139 (MQ=255) aGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGAGTCTGAGTCAGAGTATGACCTTTCGGATCGATGAATTGTAGGCTGCTGCGGTTATCTAAATc > 1:775696/1‑139 (MQ=255) ccGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCa < 1:903220/139‑1 (MQ=255) tgatTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTg < 1:129106/139‑1 (MQ=255) ttCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGAt < 2:926966/97‑1 (MQ=255) ttCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGAt > 1:926966/1‑97 (MQ=255) tCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGt > 2:442167/1‑139 (MQ=255) cccTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTtata > 2:510116/1‑139 (MQ=255) tATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGAt > 2:678425/1‑82 (MQ=255) tATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGAt < 1:678425/82‑1 (MQ=255) aCTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGc < 2:985640/139‑1 (MQ=255) aCTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGc < 1:408299/139‑1 (MQ=255) aCTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTCAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGGCTCCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGc < 2:775696/139‑1 (MQ=255) tGCTAAACGCAGCCTGGATGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGcc > 2:802090/1‑109 (MQ=255) tGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGgctgc < 1:730195/90‑1 (MQ=255) tGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGgctgc > 2:730195/1‑90 (MQ=255) tGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGcc < 1:802090/109‑1 (MQ=255) tGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGc < 1:1089929/136‑1 (MQ=255) tGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGc > 2:1089929/1‑136 (MQ=255) tGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAg > 1:549919/1‑139 (MQ=255) aGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGcc < 1:442167/139‑1 (MQ=255) aGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTg > 1:379731/1‑93 (MQ=255) aGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTg < 2:379731/93‑1 (MQ=255) cgcgTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGcc > 1:360378/1‑126 (MQ=255) cgcgTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGcc < 2:360378/126‑1 (MQ=255) gcgTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGc > 1:585164/1‑104 (MQ=255) gcgTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGc < 2:585164/104‑1 (MQ=255) tGAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCg < 2:402551/139‑1 (MQ=255) gAGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCgg > 1:378326/1‑139 (MQ=255) aGTCAGAGTATGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGc > 2:203930/1‑139 (MQ=255) tGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGt < 2:508608/67‑1 (MQ=255) tGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGt > 1:508608/1‑67 (MQ=255) tGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAg < 1:1034275/125‑1 (MQ=255) tGACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAg > 2:1034275/1‑125 (MQ=255) gACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTg < 1:1159805/55‑1 (MQ=255) gACCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTg > 2:1159805/1‑55 (MQ=255) aCCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGc < 2:86726/75‑1 (MQ=255) aCCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGc > 1:86726/1‑75 (MQ=255) aCCTTTCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACg > 2:255422/1‑139 (MQ=255) ttCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCt < 2:298669/49‑1 (MQ=255) ttCAGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGc < 1:674140/71‑1 (MQ=255) | CAGACGCACACGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACG > NC_000913/2597769‑2598035 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |