| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,827,351 | G→A | *320* (TAG→TAA) | srlE → | glucitol/sorbitol‑specific enzyme IIB component of PTS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,827,351 | 0 | G | A | 92.3% | 63.6 / ‑1.0 | 26 | *320* (TAG→TAA) | srlE | glucitol/sorbitol‑specific enzyme IIB component of PTS |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (10/14); total (11/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.95e-01 | |||||||||||
GTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCTGCGGACCTCGAAGAGTATTGCTTC > NC_000913/2827217‑2827484 | gtcggtTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGc < 1:1194094/139‑1 (MQ=255) gTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACAt < 1:196892/139‑1 (MQ=255) gCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGAc < 2:83292/139‑1 (MQ=255) gCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGtt > 2:162697/1‑139 (MQ=255) gCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGttatttat < 2:474471/139‑1 (MQ=255) gTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTTGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCAtt < 1:162697/139‑1 (MQ=255) gTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCAtt < 1:641662/139‑1 (MQ=255) gTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGcc > 2:521966/1‑139 (MQ=255) ttAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAg < 1:521966/139‑1 (MQ=255) gcgcgcCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCg > 1:670296/1‑70 (MQ=255) gcgcgcCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCg < 2:670296/70‑1 (MQ=255) gcgcgcCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCAc < 2:1040560/92‑1 (MQ=255) gcgcgcCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCAc > 1:1040560/1‑92 (MQ=255) cgcgcCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCtcat < 1:313740/139‑1 (MQ=255) tACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTg > 1:786301/1‑139 (MQ=255) cGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGgcgc > 2:962758/1‑139 (MQ=255) gCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGc < 1:310677/90‑1 (MQ=255) gCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGc > 2:310677/1‑90 (MQ=255) cTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGa > 1:1662/1‑60 (MQ=255) cTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGa < 2:1662/60‑1 (MQ=255) cTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGa < 1:1133251/111‑1 (MQ=255) cTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGa > 2:1133251/1‑111 (MQ=255) tctcCGGTTTTATCTATCAATAAAGGCTGAAACATGAc < 2:293365/38‑1 (MQ=255) tctcCGGTTTTATCTATCAATAAAGGCTGAAACATGAc > 1:293365/1‑38 (MQ=38) tctcCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCTGCGGAcc < 1:905549/139‑1 (MQ=255) cAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCTGCGGACCTCGAAGAGTATTGCttc > 2:386293/1‑139 (MQ=255) | GTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCTGCGGACCTCGAAGAGTATTGCTTC > NC_000913/2827217‑2827484 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |