Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,842,573	C→T	*157* (TAG→TAA)	hycI ←	protease involved in processing C‑terminal end of HycE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,842,573	0	C	T	100.0%	59.8 / NA	20	*157* (TAG→TAA)	hycI	protease involved in processing C‑terminal end of HycE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/9);  total (11/9)

TTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGATATCCGGCTGAA  >  NC_000913/2842444‑2842706                                                                                                                                  |                                                                                                                                      ttATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCATAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAATTACtcttct                                                                                                                              >  1:772546/1‑139 (MQ=255)                      cGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAATTACTCTTCTTCCACCGCTAACTGCGCGAAg                                                                                                         <  2:772546/139‑1 (MQ=255)                        aaCCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAATTACTCTTCTTCCACCGCTAACTGCGCGAAgcc                                                                                                       >  2:903908/1‑139 (MQ=255)                                       aTAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAATTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCa                                                                                        <  1:903908/139‑1 (MQ=255)                                                   caTCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAATTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCg                                                                            <  1:314731/139‑1 (MQ=255)                                                                       ggCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAATTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCg                                                        >  1:478388/1‑139 (MQ=255)                                                                       ggCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAATTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCg                                                        >  2:124084/1‑139 (MQ=255)                                                                               agCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAATTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTa                                                >  2:385140/1‑139 (MQ=255)                                                                               agCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAATTACTCTTCTTCCACCCCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTa                                                >  2:758878/1‑139 (MQ=255)                                                                                     ttCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAATTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGc                                          <  2:44654/139‑1 (MQ=255)                                                                                           ccATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAATTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTc                                    <  1:124084/139‑1 (MQ=255)                                                                                                aaGAACATCCCTGTCCTGATTCCTTAATGAAAAATTACTCTTCTTCCCCCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCgg                               <  1:385140/139‑1 (MQ=255)                                                                                                    aCATCCCTGTCCTGATTCCTTAATGAAAAATTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATttccttcc                                                                                          >  2:724007/1‑76 (MQ=255)                                                                                                    aCATCCCTGTCCTGATTCCTTAATGAAAAATTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATttccttcc                                                                                          <  1:724007/76‑1 (MQ=255)                                                                                                             tCCTGATTCATTAATGAAAAATTACTCTTCTTCCTCCGCTAACTGCTCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTGCCGCATCTTTAATCTGCGGGGTAATCGGGTAGTAAAAGCcg                  >  1:1081896/1‑139 (MQ=255)                                                                                                                  aTTCCTTAATGAAAAATTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCt                                         >  1:879363/1‑111 (MQ=255)                                                                                                                  aTTCCTTAATGAAAAATTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCt                                         <  2:879363/111‑1 (MQ=255)                                                                                                                     ccTTAATGAAAAATTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGATATc          <  1:758878/139‑1 (MQ=255)                                                                                                                            gAAAAATTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGATATCCGGCTGa   >  2:1177933/1‑139 (MQ=255)                                                                                                                             aaaaaTTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGATATCCGGCTGaa  >  2:603482/1‑139 (MQ=255)                                                                                                                                  |                                                                                                                                      TTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGATATCCGGCTGAA  >  NC_000913/2842444‑2842706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑