Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,871,264	G→A	P14L (CCG→CTG)	truD ←	tRNA(Glu) pseudouridine(13) synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,871,264	0	G	A	100.0%	128.5 / NA	40	P14L (CCG→CTG)	truD	tRNA(Glu) pseudouridine(13) synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/21);  total (19/21)

GCCACAAAACGGGTATTGCAGCCGTTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTC  >  NC_000913/2871129‑2871378                                                                                                                                        |                                                                                                                   gCCACAAAACGGGTATTGCAGCCGTTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGtt                                                                                                                 <  2:1170163/139‑1 (MQ=255)          cGGGTATTGCAGCCGTTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGa                                                                                                        <  1:1021042/139‑1 (MQ=255)             gTATTGCAGCCGTTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGt                                                                                                     >  1:1148494/1‑139 (MQ=255)             gTATTGCAGCCGTTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGt                                                                                                     <  2:1148494/139‑1 (MQ=255)                                      tAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCAt                                                                            >  2:627154/1‑139 (MQ=255)                                          cAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTttg                                                                        <  2:431629/139‑1 (MQ=255)                                            gAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTa                                                                                                    >  2:855963/1‑109 (MQ=255)                                            gAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTa                                                                                                    <  1:855963/109‑1 (MQ=255)                                                       ccTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAg                                                                                                  >  1:267407/1‑100 (MQ=255)                                                       ccTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCaaa                                                                                     >  1:276589/1‑113 (MQ=255)                                                       ccTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCaaa                                                                                     <  2:276589/113‑1 (MQ=255)                                                       ccTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAg                                                           >  1:1013524/1‑139 (MQ=255)                                                       ccTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCAGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAg                                                                                                  <  2:267407/100‑1 (MQ=255)                                                           cACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGa                                                                                                        >  1:1151612/1‑90 (MQ=255)                                                           cACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGa                                                                                                        <  2:1151612/90‑1 (MQ=255)                                                             ccATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAgcgc                                                     <  2:1013524/139‑1 (MQ=255)                                                                tCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTaa                                                                                                   <  1:815363/90‑1 (MQ=255)                                                                tCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGAATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTaa                                                                                                   >  2:815363/1‑90 (MQ=255)                                                                  aGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAg                                                        >  2:440163/1‑131 (MQ=255)                                                                  aGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAg                                                        <  1:440163/131‑1 (MQ=255)                                                                  aGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCg                                                >  1:368566/1‑139 (MQ=255)                                                                     cTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTc                                                                                  >  2:22484/1‑102 (MQ=255)                                                                     cTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTc                                                                                  <  1:22484/102‑1 (MQ=255)                                                                      tCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTc                                            <  2:368566/139‑1 (MQ=255)                                                                          aGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAg                                                                                                  <  1:563083/81‑1 (MQ=255)                                                                          aGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAg                                                                                                  >  2:563083/1‑81 (MQ=255)                                                                            cccAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGc                                      <  1:627154/139‑1 (MQ=255)                                                                            cccAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGc                                      <  2:549393/139‑1 (MQ=255)                                                                                  tCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATccc                                <  2:363434/139‑1 (MQ=255)                                                                                            ccacAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGt                      >  2:190826/1‑139 (MQ=255)                                                                                                      ttCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAg                                                                                                  <  2:622054/53‑1 (MQ=255)                                                                                                      ttCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAg                                                                                                  >  1:622054/1‑53 (MQ=255)                                                                                                        ccGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATccc          >  2:1024655/1‑139 (MQ=255)                                                                                                              tGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTttg                                                                        <  2:1144128/71‑1 (MQ=255)                                                                                                              tGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTttg                                                                        >  1:1144128/1‑71 (MQ=255)                                                                                                              tGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGt                      <  2:1188855/121‑1 (MQ=255)                                                                                                              tGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGt                      >  1:1188855/1‑121 (MQ=255)                                                                                                              tGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGtttt    >  1:507589/1‑139 (MQ=255)                                                                                                                gCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTc  <  1:190826/139‑1 (MQ=255)                                                                                                                gCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGATGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGttcttc  >  2:446963/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                   GCCACAAAACGGGTATTGCAGCCGTTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTC  >  NC_000913/2871129‑2871378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑